Identification
Name:DNA helicase II
Synonyms:Not Available
Gene Name:uvrD
Enzyme Class:
Biological Properties
General Function:Replication, recombination and repair
Specific Function:Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATP-dependent DNA helicase activity
binding
catalytic activity
DNA binding
DNA helicase activity
helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleic acid binding
nucleoside binding
nucleoside-triphosphatase activity
purine nucleoside binding
pyrophosphatase activity
Process
cellular macromolecule metabolic process
cellular process
DNA conformation change
DNA duplex unwinding
DNA geometric change
DNA metabolic process
DNA repair
DNA unwinding involved in replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>2163 bp
ATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCG
CCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTG
CATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTG
ACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACG
AGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCG
CACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTA
CGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGG
CAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGCATCATATTCAA
AGCTACGGTAATCCGGTGGAGCAGACCTGGCAGAAGGTGTATCAGGCGTATCAGGAAGCG
TGTGACCGCGCGGGCCTGGTGGACTTCGCCGAGCTGCTGCTGCGCGCTCACGAGTTGTGG
CTTAACAAGCCGCATATCCTGCAACACTACCGCGAACGTTTTACCAATATCCTGGTGGAC
GAATTCCAGGATACCAACAACATTCAGTACGCGTGGATCCGCCTGCTGGCGGGCGACACC
GGCAAAGTGATGATCGTCGGTGATGACGACCAGTCAATCTACGGCTGGCGCGGGGCGCAG
GTGGAGAATATTCAGCGTTTCCTTAATGATTTCCCCGGTGCCGAAACTATTCGTCTGGAG
CAAAACTACCGCTCTACCAGCAATATTCTGAGCGCCGCTAACGCCCTGATTGAAAACAAT
AACGGGCGTCTGGGTAAAAAACTGTGGACCGATGGCGCGGACGGTGAGCCTATTTCCCTC
TATTGCGCTTTTAACGAACTCGATGAAGCGCGTTTTGTGGTTAACCGCATCAAAACCTGG
CAGGACAACGGCGGAGCGCTTGCCGAGTGCGCCATTCTCTACCGCAGCAACGCCCAGTCG
CGGGTGCTCGAAGAGGCGTTATTGCAGGCCAGTATGCCGTACCGTATTTACGGCGGGATG
CGCTTCTTCGAACGCCAGGAAATCAAAGATGCGCTCTCGTATCTGCGCCTGATTGCCAAC
CGCAACGACGACGCGGCCTTTGAGCGTGTGGTGAATACGCCAACGCGGGGTATTGGTGAC
CGGACGCTGGACGTGGTACGTCAGACATCGCGCGATCGCCAGTTAACACTCAGGCAGGCA
TGTCGTGAGCTGTTGCAGGAAAAAGCCCTCGCCGGGCGAGCTGCCAGCGCCTTGCAGCGA
TTTATGGAATTAATCGACGCCTTAGCGCAGGAAACTGCCGATATGCCGCTGCATGTACAG
ACTGACCGGGTAATTAAAGACTCCGGCCTGCGTACCATGTATGAGCAGGAGAAGGGCGAA
AAAGGTCAGACGCGTATCGAAAACTTAGAGGAACTGGTGACGGCAACGCGCCAGTTCAGC
TACAACGAAGAAGACGAAGATTTAATGCCGCTGCAGGCGTTCCTCTCCCATGCGGCACTG
GAAGCAGGTGAAGGGCAGGCGGATACCTGGCAGGATGCGGTGCAGTTGATGACGCTACAC
TCGGCGAAAGGCCTGGAGTTCCCGCAGGTGTTTATCGTTGGTATGGAAGAGGGCATGTTC
CCAAGCCAGATGTCGCTGGATGAAGGCGGGCGTCTGGAAGAAGAACGCCGTCTGGCCTAC
GTTGGCGTAACCCGCGCGATGCAGAAACTGACGCTGACCTACGCGGAAACCCGCCGTCTG
TATGGTAAAGAGGTTTACCATCGCCCGTCGCGCTTTATCGGCGAGCTGCCGGAAGAGTGT
GTGGAAGAGGTGCGCCTGCGCGCCACGGTAAGCCGCCCGGTCAGCCATCAGCGGATGGGT
ACGCCGATGGTCGAGAACGACAGCGGCTACAAGCTCGGCCAGCGCGTACGCCACGCTAAG
TTTGGTGAAGGCACCATTGTCAATATGGAAGGCAGCGGTGAGCATAGCCGTTTGCAGGTG
GCATTTCAGGGCCAGGGTATTAAATGGCTGGTGGCGGCATACGCCCGGCTGGAGTCGGTG
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:720
Protein Molecular Weight:81989
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA helicase II
MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAV
TFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQL
RLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEA
CDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDT
GKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENN
NGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQS
RVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGD
RTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQ
TDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAAL
EAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAY
VGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMG
TPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV
References
External Links:
ResourceLink
Uniprot ID:P03018
Uniprot Name:UVRD_ECOLI
GenBank Gene ID:D00069
Genebank Protein ID:216673
CCDB:UVRD_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Colloms, S. D., Sykora, P., Szatmari, G., Sherratt, D. J. (1990). "Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases." J Bacteriol 172:6973-6980. Pubmed: 2254268
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Easton, A. M., Kushner, S. R. (1983). "Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation." Nucleic Acids Res 11:8625-8640. Pubmed: 6324092
  • Finch, P. W., Emmerson, P. T. (1984). "The nucleotide sequence of the uvrD gene of E. coli." Nucleic Acids Res 12:5789-5799. Pubmed: 6379604
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Washburn, B. K., Kushner, S. R. (1993). "Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli." J Bacteriol 175:341-350. Pubmed: 8419285
  • Yamamoto, Y., Ogawa, T., Shinagawa, H., Nakayama, T., Matsuo, H., Ogawa, H. (1986). "Determination of the initiation sites of transcription and translation of the uvrD gene of Escherichia coli." J Biochem 99:1579-1590. Pubmed: 2943729