Identification
Name:Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]
Synonyms:
  • Ap4A hydrolase
  • Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase
  • Diadenosine tetraphosphatase
Gene Name:apaH
Enzyme Class:
Biological Properties
General Function:Involved in bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
Specific Function:Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP
Cellular Location:Cytoplasmic
SMPDB Pathways:
  • Operon: 16S ribosomal RNA modification & chaperone PW001877
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb2.0Thumb+2.0Thumb
1.0Thumb+1.0Thumb2.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb2.0Thumb+2.0Thumb
1.0Thumb+1.0Thumb2.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb2.0Thumb+2.0Thumb
1.0Thumb+1.0Thumb2.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01211Diadenosine tetraphosphateMetaboCard
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21264P1,P4-Bis(5'-guanosyl) tetraphosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
bis(5'-nucleosyl)-tetraphosphatase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleotide diphosphatase activity
pyrophosphatase activity
Gene Properties
Blattner:b0049
Gene OrientationCounterclockwise
Centisome Percentage:1.09
Left Sequence End50380
Right Sequence End51222
Gene Sequence:
>843 bp
ATGGCGACATACCTTATTGGCGACGTTCATGGTTGTTACGATGAACTGATCGCATTGCTG
CATAAAGTAGAATTTACCCCTGGGAAAGATACCCTCTGGCTGACGGGCGATCTGGTCGCG
CGCGGGCCGGGTTCGCTGGATGTTCTGCGCTATGTGAAATCCTTAGGCGACAGCGTACGT
CTGGTGCTGGGCAATCACGATCTGCATCTGCTGGCGGTATTTGCCGGGATCAGCCGCAAT
AAACCGAAAGATCGCCTGACACCGCTGCTGGAAGCGCCGGATGCCGACGAGCTGCTTAAC
TGGCTGCGGCGCCAGCCTCTGCTGCAAATCGACGAAGAGAAAAAGCTGGTGATGGCCCAC
GCAGGGATCACGCCGCAGTGGGATCTGCAGACCGCCAAAGAGTGCGCACGCGATGTAGAA
GCGGTGCTATCGAGTGACTCCTATCCCTTCTTTCTTGATGCCATGTACGGCGATATGCCA
AATAACTGGTCACCGGAATTGCGGGGGCTGGGAAGACTGCGTTTTATCACCAACGCTTTT
ACCCGTATGCGTTTTTGCTTCCCGAACGGTCAACTGGATATGTACAGCAAAGAATCGCCG
GAAGAGGCCCCTGCCCCACTGAAACCGTGGTTTGCGATTCCTGGCCCTGTCGCTGAAGAA
TACAGCATCGCCTTTGGTCACTGGGCATCGCTGGAGGGCAAAGGTACGCCGGAAGGTATA
TACGCGCTGGATACCGGCTGCTGCTGGGGTGGTACATTAACCTGCCTGCGCTGGGAAGAT
AAACAGTATTTTGTCCAGCCGTCGAACCGGCATAAGGATTTGGGCGAAGCGGCGGCGTCT
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:280
Protein Molecular Weight:31296
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]
MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPGSLDVLRYVKSLGDSVR
LVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWLRRQPLLQIDEEKKLVMAH
AGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMPNNWSPELRGLGRLRFITNAF
TRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVAEEYSIAFGHWASLEGKGTPEGI
YALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGEAAAS
References
External Links:
ResourceLink
Uniprot ID:P05637
Uniprot Name:APAH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674302
Ecogene ID:EG10048
Ecocyc:EG10048
ColiBase:b0049
Kegg Gene:b0049
EchoBASE ID:EB0046
CCDB:APAH_ECOLI
BacMap:16128043
General Reference:
  • Blanchin-Roland, S., Blanquet, S., Schmitter, J. M., Fayat, G. (1986). "The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA." Mol Gen Genet 205:515-522. Pubmed: 3031429
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Guranowski, A., Jakubowski, H., Holler, E. (1983). "Catabolism of diadenosine 5',5"'-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5',5"'-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12." J Biol Chem 258:14784-14789. Pubmed: 6317672
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901