Ferrienterobactin receptor (P05825)

Identification
Name:Ferrienterobactin receptor
Synonyms:
  • Enterobactin outer-membrane receptor
Gene Name:fepA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in receptor activity
Specific Function:This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D
Cellular Location:Cell outer membrane; Multi-pass membrane protein
SMPDB Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21372Ferric enterobactinMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
Function
binding
cation binding
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion binding
ion transmembrane transporter activity
iron ion binding
iron ion transmembrane transporter activity
metal ion binding
molecular transducer activity
receptor activity
siderophore-iron transmembrane transporter activity
signal transducer activity
substrate-specific transmembrane transporter activity
transition metal ion binding
transmembrane transporter activity
transporter activity
Process
establishment of localization
siderophore transport
transport
Gene Properties
Blattner:b0584
Gene OrientationCounterclockwise
Centisome Percentage:13.14
Left Sequence End609477
Right Sequence End611717
Gene Sequence:
>2241 bp
ATGAACAAGAAGATTCATTCCCTGGCCTTGTTGGTCAATCTGGGGATTTATGGGGTAGCG
CAGGCACAAGAGCCGACCGATACTCCTGTTTCACATGACGATACTATTGTCGTTACCGCC
GCCGAGCAGAACTTACAGGCGCCTGGCGTTTCGACCATCACCGCAGATGAAATCCGCAAA
AACCCGGTTGCCCGCGATGTGTCGAAGATCATCCGTACCATGCCAGGCGTTAACCTGACC
GGTAACTCCACCAGTGGTCAGCGTGGGAATAACCGACAGATTGATATTCGCGGTATGGGT
CCGGAAAACACGCTGATTTTGATTGACGGCAAGCCGGTAAGCAGCCGTAACTCGGTGCGT
CAGGGCTGGCGTGGCGAGCGCGATACCCGTGGTGATACTTCCTGGGTGCCACCTGAAATG
ATTGAACGTATTGAAGTTCTGCGTGGTCCGGCAGCTGCGCGTTATGGCAACGGCGCGGCG
GGCGGCGTGGTTAACATCATTACCAAAAAAGGCAGCGGCGAGTGGCACGGCTCCTGGGAC
GCATATTTCAATGCGCCAGAACATAAAGAGGAAGGTGCCACCAAACGCACTAACTTTAGC
CTGACCGGTCCGCTGGGCGACGAATTCAGCTTCCGTTTGTATGGCAACCTCGACAAAACC
CAGGCTGACGCGTGGGATATCAACCAGGGCCATCAGTCCGCGCGTGCCGGAACGTATGCC
ACGACGTTACCAGCCGGGCGCGAAGGGGTAATCAACAAAGATATTAATGGCGTGGTGCGC
TGGGATTTCGCGCCATTGCAATCGCTGGAACTGGAAGCAGGTTACAGCCGCCAGGGTAAC
CTGTATGCGGGCGACACCCAGAATACCAACTCCGATTCCTATACCCGCTCGAAATATGGC
GATGAAACCAACCGTCTGTATCGCCAGAACTACGCGCTGACCTGGAACGGTGGCTGGGAT
AACGGCGTGACCACCAGCAACTGGGTGCAGTACGAACACACCCGTAACTCGCGTATTCCG
GAAGGTCTGGCGGGCGGTACCGAAGGGAAATTTAACGAAAAAGCGACACAGGATTTCGTC
GATATCGATCTTGATGACGTGATGCTGCACAGCGAAGTTAACCTGCCGATTGATTTCCTC
GTTAACCAGACGCTGACGCTGGGTACGGAGTGGAATCAGCAACGGATGAAGGACTTAAGT
TCCAACACCCAGGCACTGACCGGAACGAATACCGGTGGCGCTATTGATGGCGTGAGTACC
ACCGACCGTAGCCCGTATTCAAAAGCAGAAATTTTCTCGCTGTTTGCCGAAAACAACATG
GAGCTGACTGACAGCACCATCGTAACGCCGGGGCTGCGTTTCGATCATCACAGTATTGTC
GGCAATAACTGGAGCCCGGCGCTGAACATATCGCAAGGTTTAGGCGATGACTTCACGCTG
AAAATGGGCATCGCCCGTGCTTATAAAGCGCCGAGCCTGTACCAGACTAACCCGAACTAC
ATTCTCTACAGTAAAGGTCAGGGTTGCTATGCCAGCGCGGGCGGCTGCTATCTGCAAGGT
AACGATGACCTGAAAGCAGAAACCAGCATCAACAAAGAGATTGGTCTGGAGTTCAAACGC
GACGGGTGGCTGGCGGGCGTCACCTGGTTCCGTAACGATTATCGCAATAAGATTGAAGCG
GGCTATGTGGCTGTAGGGCAAAACGCAGTCGGCACCGATCTCTATCAGTGGGATAACGTG
CCGAAAGCGGTGGTTGAAGGTCTGGAAGGATCGTTAAACGTACCGGTTAGCGAAACGGTG
ATGTGGACCAATAACATCACTTATATGCTGAAGAGTGAAAACAAAACCACGGGCGACCGT
TTGTCGATCATCCCGGAGTATACGTTGAACTCAACGCTGAGCTGGCAGGCACGGGAAGAT
TTGTCGATGCAAACGACCTTCACCTGGTACGGCAAGCAGCAGCCGAAGAAGTACAACTAT
AAAGGTCAGCCAGCGGTTGGACCGGAAACCAAAGAAATTAGTCCTTACAGCATTGTTGGC
CTGAGCGCGACCTGGGATGTGACGAAGAATGTCAGTCTGACCGGCGGCGTGGACAATCTG
TTCGACAAACGTTTGTGGCGTGCGGGTAATGCCCAGACCACGGGCGATTTGGCAGGGGCC
AACTATATCGCCGGTGCCGGGGCGTATACCTATAACGAGCCGGGACGTACGTGGTATATG
AGCGTAAACACCCACTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:746
Protein Molecular Weight:82106
Protein Theoretical pI:5
PDB File:1FEP
Signaling Regions:
  • 1-22
Transmembrane Regions:
  • None
Protein Sequence:
>Ferrienterobactin receptor
MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQNLQAPGVSTITADEIRK
NPVARDVSKIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVSSRNSVR
QGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAAGGVVNIITKKGSGEWHGSWD
AYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLDKTQADAWDINQGHQSARAGTYA
TTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYSRQGNLYAGDTQNTNSDSYTRSKYG
DETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHTRNSRIPEGLAGGTEGKFNEKATQDFV
DIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWNQQRMKDLSSNTQALTGTNTGGAIDGVST
TDRSPYSKAEIFSLFAENNMELTDSTIVTPGLRFDHHSIVGNNWSPALNISQGLGDDFTL
KMGIARAYKAPSLYQTNPNYILYSKGQGCYASAGGCYLQGNDDLKAETSINKEIGLEFKR
DGWLAGVTWFRNDYRNKIEAGYVAVGQNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETV
MWTNNITYMLKSENKTTGDRLSIIPEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNY
KGQPAVGPETKEISPYSIVGLSATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGA
NYIAGAGAYTYNEPGRTWYMSVNTHF
References
External Links:
ResourceLink
Uniprot ID:P05825
Uniprot Name:FEPA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062212
PDB ID:1FEP
Ecogene ID:EG10293
Ecocyc:EG10293
ColiBase:b0584
Kegg Gene:b0584
EchoBASE ID:EB0289
CCDB:FEPA_ECOLI
BacMap:16128567
General Reference:
  • Armstrong, S. K., Francis, C. L., McIntosh, M. A. (1990). "Molecular analysis of the Escherichia coli ferric enterobactin receptor FepA." J Biol Chem 265:14536-14543. Pubmed: 2201687
  • Armstrong, S. K., Pettis, G. S., Forrester, L. J., McIntosh, M. A. (1989). "The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product." Mol Microbiol 3:757-766. Pubmed: 2526281
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Buchanan, S. K., Smith, B. S., Venkatramani, L., Xia, D., Esser, L., Palnitkar, M., Chakraborty, R., van der Helm, D., Deisenhofer, J. (1999). "Crystal structure of the outer membrane active transporter FepA from Escherichia coli." Nat Struct Biol 6:56-63. Pubmed: 9886293
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lundrigan, M. D., Kadner, R. J. (1986). "Nucleotide sequence of the gene for the ferrienterochelin receptor FepA in Escherichia coli. Homology among outer membrane receptors that interact with TonB." J Biol Chem 261:10797-10801. Pubmed: 3015941
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pettis, G. S., Brickman, T. J., McIntosh, M. A. (1988). "Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter." J Biol Chem 263:18857-18863. Pubmed: 2974033