Vitamin B12 transporter BtuB (P06129)

Identification
Name:Vitamin B12 transporter BtuB
Synonyms:
  • Cobalamin receptor
  • Outer membrane cobalamin translocator
Gene Name:btuB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in receptor activity
Specific Function:Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein tonB. Is also a receptor for bacteriophages BF23 and C1, and for A and E colicins
Cellular Location:Cell outer membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB02086AdenosylcobalaminMetaboCard
ECMDB04040Cob(I)alaminMetaboCard
ECMDB06902CobinamideMetaboCard
ECMDB21386CyanocobalaminMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
Function
molecular transducer activity
receptor activity
signal transducer activity
transporter activity
Process
cobalamin transport
establishment of localization
transport
vitamin transport
Gene Properties
Blattner:b3966
Gene OrientationClockwise
Centisome Percentage:89.70
Left Sequence End4161662
Right Sequence End4163506
Gene Sequence:
>1845 bp
ATGAATTCACTACGTCCGGAATTATTAGAACTGACACCGCAGGCCCTGACGGCGTTAAGC
AATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATC
AGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCG
AACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCAT
CGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAA
GAAGAAGAGTGGGATCCGGCAATCTGGCTGGAAGAACTGGCTACCCTTCCCGATGCTACC
CGCAAACGCGCACAGGCGCTGGTCGCTAAAGGCATCACCATTGAGTTGTTCTGTGCGCCG
GGTGAAATTCCCTCTGCCCGCTTACCGATGAGCGATGTGCGTTTTTATTCCCGCAGCAGT
ATTCGTTTCGCCCGTTGTGATTGTATTGAAGGCACACTTTGCGAACATGTCGTACTGGCG
GTACAGGCCTTCGTCGAGGCCAAAGCGCAGCAAGCAGAATTTAACCATTTAATCTGGCAG
ATGCGCAGCGAACACGTCACATCATCTGACGATCCGTTTGCCAGCGAAGAAGGCAACGCG
TGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCG
CTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGACCTGCAACTGG
CGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGC
GCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTA
AATTGCGCACAAGAGATGGCCCGGCGCGACAGTATTGGTGAAGTTCCTCCTGTGCCGTGG
CGCACGGTCGTTGGCTCTGGCATTGCCGGAGAAGCAAAGCTTGATCATCTGCGGCTGGTG
TCTTTAGGTATGCGTTGCTGGCAGGATATTGAGCATTATGGTTTACGCATCTGGTTTACC
GATCCCGACACCGGCAGTATTTTGCACCTTTCGCGCAGTTGGCCGCGAAGTGAACAGGAA
AACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGG
CAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGC
AACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGGCAAATGTTGAGCGCGCCG
TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGC
ATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCG
CTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAGGTCATTAGCGGCGAAGGGGAA
GATAATGTGCTGACGTTATCATTACCAGCCTCAGCCAGCGCACCTTATGCCGTTGAACGC
ATGGCGGCGCTTTTGCAACAAACAGACGACCCCGTGTGTCTGGTTTCTGGCTTTGTCAGT
TTTGTTGAAGGGCAATTGACACTGGAACCACGGGTGATGATGACAAAAACCCGTGCCTGG
GCGCTGGACGCAGAAACTACGCCTGTGGCACCGCTACCTTCTGCCAGCGTTTTGCCTGTG
CCGTCTACTGCTCATCAGTTGCTGATACGCTGCCAGGCGTTACTTATTCAACTGCTCCAT
AACGGCTGGCGCTATCAGGAACAGAGTGCTATTGGTCAGGCATAG
Protein Properties
Pfam Domain Function:
Protein Residues:614
Protein Molecular Weight:68407
Protein Theoretical pI:5
PDB File:1UJW
Signaling Regions:
  • 1-20
Transmembrane Regions:
  • 158-165
  • 169-178
  • 184-195
  • 217-227
  • 232-248
  • 263-277
  • 279-296
  • 309-325
  • 328-337
  • 353-369
  • 371-381
  • 385-400
  • 403-417
  • 434-443
  • 449-458
  • 473-490
  • 494-509
  • 517-529
  • 535-550
  • 558-572
  • 585-596
  • 602-614
Protein Sequence:
>Vitamin B12 transporter BtuB
MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLAPTTVVTRQDIDRWQST
SVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDGVRLNLAGVSGSADLSQFP
IALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEISAGWGSNSYQNYDVSTQQQL
GDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFLSKTLYGALEHNFTDAWSGFVRG
YGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRYNGELIKSQLITSYSHSKDYNYDPH
YGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAGVDWQKQTTTPGTGYVEDGYDQRNTGI
YLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQ
LYGFYGNPNLDPEKSKQWEGAFEGLTAGVNWRISGYRNDVSDLIDYDDHTLKYYNEGKAR
IKGVEATANFDTGPLTHTVSYDYVDARNAITDTPLLRRAKQQVKYQLDWQLYDFDWGITY
QYLGTRYDKDYSSYPYQTVKMGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQ
TAGREYTLSGSYTF
References
External Links:
ResourceLink
Uniprot ID:P06129
Uniprot Name:BTUB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675235
PDB ID:1UJW
Ecogene ID:EG10126
Ecocyc:EG10126
ColiBase:b3966
Kegg Gene:b3966
CCDB:BTUB_ECOLI
BacMap:16131804
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cadieux, N., Kadner, R. J. (1999). "Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter." Proc Natl Acad Sci U S A 96:10673-10678. Pubmed: 10485884
  • Chimento, D. P., Kadner, R. J., Wiener, M. C. (2003). "The Escherichia coli outer membrane cobalamin transporter BtuB: structural analysis of calcium and substrate binding, and identification of orthologous transporters by sequence/structure conservation." J Mol Biol 332:999-1014. Pubmed: 14499604
  • Chimento, D. P., Mohanty, A. K., Kadner, R. J., Wiener, M. C. (2003). "Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli." Acta Crystallogr D Biol Crystallogr 59:509-511. Pubmed: 12595710
  • Chimento, D. P., Mohanty, A. K., Kadner, R. J., Wiener, M. C. (2003). "Substrate-induced transmembrane signaling in the cobalamin transporter BtuB." Nat Struct Biol 10:394-401. Pubmed: 12652322
  • Coggshall, K. A., Cadieux, N., Piedmont, C., Kadner, R. J., Cafiso, D. S. (2001). "Transport-defective mutations alter the conformation of the energy-coupling motif of an outer membrane transporter." Biochemistry 40:13964-13971. Pubmed: 11705387
  • Dougherty, T. J., Thanassi, J. A., Pucci, M. J. (1993). "The Escherichia coli mutant requiring D-glutamic acid is the result of mutations in two distinct genetic loci." J Bacteriol 175:111-116. Pubmed: 8093236
  • Gudmundsdottir, A., Bell, P. E., Lundrigan, M. D., Bradbeer, C., Kadner, R. J. (1989). "Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport." J Bacteriol 171:6526-6533. Pubmed: 2687240
  • Gustafsson, C., Lindstrom, P. H., Hagervall, T. G., Esberg, K. B., Bjork, G. R. (1991). "The trmA promoter has regulatory features and sequence elements in common with the rRNA P1 promoter family of Escherichia coli." J Bacteriol 173:1757-1764. Pubmed: 1999392
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Heller, K., Kadner, R. J. (1985). "Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli." J Bacteriol 161:904-908. Pubmed: 3882670
  • Kurisu, G., Zakharov, S. D., Zhalnina, M. V., Bano, S., Eroukova, V. Y., Rokitskaya, T. I., Antonenko, Y. N., Wiener, M. C., Cramer, W. A. (2003). "The structure of BtuB with bound colicin E3 R-domain implies a translocon." Nat Struct Biol 10:948-954. Pubmed: 14528295
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Nou, X., Kadner, R. J. (1998). "Coupled changes in translation and transcription during cobalamin-dependent regulation of btuB expression in Escherichia coli." J Bacteriol 180:6719-6728. Pubmed: 9852020