Identification |
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Name: | GTPase Era |
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Synonyms: | Not Available |
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Gene Name: | era |
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Enzyme Class: | Not Available |
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Biological Properties |
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General Function: | ribosomal small subunit biogenesis |
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Specific Function: | An essential GTPase that binds both GDP and GTP, with nucleotide exchange occurring on the order of seconds whereas hydrolysis occurs on the order of minutes. Plays a role in numerous processes, including cell cycle regulation, energy metabolism, as a chaperone for 16S rRNA processing and 30S ribosomal subunit biogenesis. Its presence in the 30S subunit may prevent translation initiation. Seems to be critical for maintaining cell growth and cell divison rates; a dramatic reduction in Era protein levels temporarily arrests cell growth just before cytokinesis (at the predivisional two-cell stage) and delays cell division. Era mutant era1 suppresses some temperature-sensitive mutations that affect DNA replication and chromosome partitioning and segregation. The dominant-negative Era-de mutant which is missing residues in a putative effector region, is unable to complement the disruption mutant; upon overproduction it shows a significant decrease in cell viability and a synthetic lethal phenotype in the presence of acetate. Era function probably overlaps RbfA. Binds to the pre-30S subunit through several stages of protein assembly. |
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Cellular Location: | Not Available |
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SMPDB Pathways: | Not Available |
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KEGG Pathways: | Not Available |
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Metabolites: | |
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GO Classification: | Function |
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cytoplasm | cytosol | GTP binding | GTPase activity | plasma membrane | protein phosphorylation | ribosomal small subunit binding | ribosomal small subunit biogenesis | small ribosomal subunit rRNA binding |
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Gene Properties |
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Blattner: | Not Available |
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Gene Orientation | Not Available |
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Centisome Percentage: | Not Available |
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Left Sequence End | Not Available |
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Right Sequence End | Not Available |
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Gene Sequence: | >906
atgagcatcgataaaagttactgcggatttattgccatcgtcggacgtccgaacgttggc
aaatccacattgttgaacaaactgctggggcagaaaatctccatcacttcccgcaaggcg
cagacaactcgtcaccgcattgtggggatccatactgaaggcgcgtatcaggcgatctac
gtcgatacaccgggcctgcatatggaagaaaaacgcgccattaaccgcctgatgaacaaa
gcggcgagcagctctattggcgatgttgagctggtgatttttgtcgttgaaggcacccgc
tggacgccggacgacgaaatggtgctcaacaaactgcgcgaaggcaaagcgccggtaatc
ctcgcggtgaacaaagtggacaacgtgcaggagaaagccgatctgctgccgcacctgcag
ttcctggcaagccagatgaacttcctcgatatcgtgccaatctctgccgaaaccgggctg
aatgttgacactattgcggcaatcgtgcgtaagcatctacctgaagcgactcatcacttc
ccggaagattacatcaccgatcgctcacagcgttttatggcgtctgaaatcatccgcgaa
aaactgatgcgtttcctcggcgctgaactgccgtactccgtgaccgtggagatcgaacgt
ttcgtctctaacgaacgcggtggttatgacatcaacggtttgattctcgttgagcgtgaa
gggcagaagaagatggtcattggcaacaaaggggccaagatcaaaaccatcgggattgaa
gcgcgtaaagacatgcaggaaatgttcgaagcgcctgttcaccttgaactgtgggtaaaa
gtgaaatccggttgggccgacgacgaacgcgcactgcgcagtctcggttacgttgacgat
ctttaa |
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Protein Properties |
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Pfam Domain Function: | Not Available |
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Protein Residues: | 301 |
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Protein Molecular Weight: | 33809 |
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Protein Theoretical pI: | Not Available |
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PDB File: | 1EGA |
Signaling Regions: | Not Available |
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Transmembrane Regions: | Not Available |
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Protein Sequence: | >GTPase Era
MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY
VDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVI
LAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHF
PEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVERE
GQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYVDD
L |
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References |
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External Links: | |
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General Reference: | Not Available |
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