Elongation factor G (P0A6M8)

Identification
Name:Elongation factor G
Synonyms:
  • EF-G
Gene Name:fusA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in GTPase activity
Specific Function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Component
cell part
intracellular
Function
binding
catalytic activity
GTP binding
GTPase activity
guanyl nucleotide binding
guanyl ribonucleotide binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleic acid binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleotide binding
pyrophosphatase activity
RNA binding
translation elongation factor activity
translation factor activity, nucleic acid binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
metabolic process
translational elongation
Gene Properties
Blattner:b3340
Gene OrientationCounterclockwise
Centisome Percentage:74.78
Left Sequence End3469422
Right Sequence End3471536
Gene Sequence:
>2115 bp
ATGGCTCGTACAACACCCATCGCACGCTACCGTAACATCGGTATCAGTGCGCACATCGAC
GCCGGTAAAACCACTACTACCGAACGTATTCTGTTCTACACCGGTGTAAACCATAAAATC
GGTGAAGTTCATGACGGCGCTGCAACCATGGACTGGATGGAGCAGGAGCAGGAACGTGGT
ATTACCATCACTTCCGCTGCGACTACTGCATTCTGGTCTGGTATGGCTAAGCAGTATGAG
CCGCATCGCATCAACATCATCGACACCCCGGGGCACGTTGACTTCACAATCGAAGTAGAA
CGTTCCATGCGTGTTCTCGATGGTGCGGTAATGGTTTACTGCGCAGTTGGTGGTGTTCAG
CCGCAGTCTGAAACCGTATGGCGTCAGGCAAACAAATATAAAGTTCCGCGCATTGCGTTC
GTTAACAAAATGGACCGCATGGGTGCGAACTTCCTGAAAGTTGTTAACCAGATCAAAACC
CGTCTGGGCGCGAACCCGGTTCCGCTGCAGCTGGCGATTGGTGCTGAAGAACATTTCACC
GGTGTTGTTGACCTGGTGAAAATGAAAGCTATCAACTGGAACGACGCTGACCAGGGCGTA
ACCTTCGAATACGAAGATATCCCGGCAGACATGGTTGAACTGGCTAACGAATGGCACCAG
AACCTGATCGAATCCGCAGCTGAAGCTTCTGAAGAGCTGATGGAAAAATACCTGGGTGGT
GAAGAACTGACTGAAGCAGAAATCAAAGGTGCTCTGCGTCAGCGCGTTCTGAACAACGAA
ATCATCCTGGTAACCTGTGGTTCTGCGTTCAAGAACAAAGGTGTTCAGGCGATGCTGGAT
GCGGTAATTGATTACCTGCCATCCCCGGTTGACGTACCTGCGATCAACGGTATCCTGGAC
GACGGTAAAGACACTCCGGCTGAACGTCACGCAAGTGATGACGAGCCGTTCTCTGCACTG
GCGTTCAAAATCGCTACCGACCCGTTTGTTGGTAACCTGACCTTCTTCCGTGTTTACTCC
GGTGTGGTTAACTCTGGTGATACCGTACTGAACTCCGTGAAAGCTGCACGTGAGCGTTTC
GGTCGTATCGTTCAGATGCACGCTAACAAACGTGAAGAGATCAAAGAAGTTCGCGCGGGC
GACATCGCTGCTGCTATCGGTCTGAAAGACGTAACCACTGGTGACACCCTGTGTGACCCG
GATGCGCCGATCATTCTGGAACGTATGGAATTCCCTGAGCCGGTAATCTCCATCGCAGTT
GAACCGAAAACCAAAGCTGACCAGGAAAAAATGGGTCTGGCTCTGGGCCGTCTGGCTAAA
GAAGACCCGTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGT
ATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAA
GCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGCCAGAAAGTTACCGAT
GTTGAAGGTAAACACGCGAAACAGTCTGGTGGTCGTGGTCAGTATGGTCATGTTGTTATC
GACATGTACCCGCTGGAGCCGGGTTCAAACCCGAAAGGCTACGAGTTCATCAACGACATT
AAAGGTGGTGTAATCCCTGGCGAATACATCCCGGCCGTTGATAAAGGTATCCAGGAACAG
CTGAAAGCAGGTCCGCTGGCAGGCTACCCGGTAGTAGACATGGGTATTCGTCTGCACTTC
GGTTCTTACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCC
TTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAA
GTAGAAACTCCGGAAGAGAACACCGGTGACGTTATCGGTGACTTGAGCCGTCGTCGTGGT
ATGCTCAAAGGTCAGGAATCTGAAGTTACTGGCGTTAAGATCCACGCTGAAGTACCGCTG
TCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATAC
ACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCAGGCCGTAATTGAA
GCCCGTGGTAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:704
Protein Molecular Weight:77581
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Elongation factor G
MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMDWMEQEQERG
ITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQ
PQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKTRLGANPVPLQLAIGAEEHFT
GVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAEASEELMEKYLGG
EELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILD
DGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERF
GRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAV
EPKTKADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFNVE
ANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDI
KGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIA
FKEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPL
SEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEARGK
References
External Links:
ResourceLink
Uniprot ID:P0A6M8
Uniprot Name:EFG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676701
Ecogene ID:EG10360
Ecocyc:EG10360
ColiBase:b3340
Kegg Gene:b3340
EchoBASE ID:EB0355
CCDB:EFG_ECOLI
BacMap:16131219
General Reference:
  • Alakhov, Y. B., Dovgas, N. V., Motuz, L. P., Vinokurov, L. M., Ovchinnikov, Y. A. (1981). "The primary structure of the elongation factor G from Escherichia coli: amino acid sequence of the C-terminal domain." FEBS Lett 126:183-186. Pubmed: 7016587
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Johanson, U., Hughes, D. (1992). "Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli." Gene 120:93-98. Pubmed: 1398129
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Ovchinnikov, Y. u. A., Alakhov, Y. u. B., Bundulis, Y. u. P., Bundule, M. A., Dovgas, N. V., Kozlov, V. P., Motuz, L. P., Vinokurov, L. M. (1982). "The primary structure of elongation factor G from Escherichia coli. A complete amino acid sequence." FEBS Lett 139:130-135. Pubmed: 7042386
  • Post, L. E., Nomura, M. (1980). "DNA sequences from the str operon of Escherichia coli." J Biol Chem 255:4660-4666. Pubmed: 6989816
  • Valle, M., Zavialov, A., Sengupta, J., Rawat, U., Ehrenberg, M., Frank, J. (2003). "Locking and unlocking of ribosomal motions." Cell 114:123-134. Pubmed: 12859903
  • Yokota, T., Sugisaki, H., Takanami, M., Kaziro, Y. (1980). "The nucleotide sequence of the cloned tufA gene of Escherichia coli." Gene 12:25-31. Pubmed: 7011903
  • Zengel, J. M., Archer, R. H., Lindahl, L. (1984). "The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G." Nucleic Acids Res 12:2181-2192. Pubmed: 6322136
  • Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842