Identification
Name:Integration host factor subunit alpha
Synonyms:Not Available
Gene Name:ihfA
Enzyme Class:Not Available
Biological Properties
General Function:transcription, DNA-templated
Specific Function:One of the 2 subunits of integration host factor (IHF), a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.Plays a crucial role in the lysogenic life cycle of bacteriophage lambda, as it is required not only in the recombination reaction, which inserts lambda DNA into the E.coli chromosome, but also for the synthesis of int and cI repressor, two phage proteins necessary for DNA insertion and repression, respectively. The synthesis of int and cI proteins is regulated indirectly by IHF via translational control of the lambda cII protein.Has an essential role in conjugative DNA transfer (CDT), the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then faciliates binding of TraI.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
conjugation
cytoplasm
DNA binding
DNA recombination
regulation of transcription, DNA-templated
regulation of translation
transcription, DNA-templated
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>300
atggcgcttacaaaagctgaaatgtcagaatatctgtttgataagcttgggcttagcaag
cgggatgccaaagaactggttgaactgtttttcgaagagatccgtcgcgctctggaaaac
ggcgaacaggtgaaactctctggttttggtaacttcgatctgcgtgataagaatcaacgc
ccgggacgtaacccgaaaacgggcgaggatattcccattacagcacggcgcgtggtgacc
ttcagacccgggcagaagttaaaaagccgggtcgaaaacgcttcgcccaaagacgagtaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:99
Protein Molecular Weight:11353
Protein Theoretical pI:Not Available
PDB File:1IHF
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>Integration host factor subunit alpha
MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQVKLSGFGNFDLRDKNQR
PGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE
References
External Links:
ResourceLink
Uniprot ID:P0A6X7
Uniprot Name:IHFA_ECOLI
PDB ID:1IHF
CCDB:IHFA_ECOLI
General Reference:Not Available