RNA pyrophosphohydrolase (P0A776)

Identification
Name:RNA pyrophosphohydrolase
Synonyms:
  • (Di)nucleoside polyphosphate hydrolase
  • Ap5A pyrophosphatase
Gene Name:rppH
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:Master regulator of 5'-dependent mRNA decay. Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. Preferentially hydrolyzes diadenosine penta-phosphate with ATP as one of the reaction products. Also able to hydrolyze diadenosine hexa- and tetra-phosphate. Has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP- glucose. In the meningitis causing strain E.coli K1, has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB04142PyrophosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
Gene Properties
Blattner:b2830
Gene OrientationCounterclockwise
Centisome Percentage:63.94
Left Sequence End2966469
Right Sequence End2966999
Gene Sequence:
>531 bp
ATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATG
CTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCT
GACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTC
TGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAA
GTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTG
CTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGC
TTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTG
CCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCG
CGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAG
Protein Properties
Pfam Domain Function:
Protein Residues:176
Protein Molecular Weight:20795
Protein Theoretical pI:11
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>RNA pyrophosphohydrolase
MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGESAEQAMYRELFEEVGL
SRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLLQLVSGDAEINMQTSSTPE
FDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQENTPKPQNASAYRRKRG
References
External Links:
ResourceLink
Uniprot ID:P0A776
Uniprot Name:RPPH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674301
Ecogene ID:EG13091
Ecocyc:EG13091
ColiBase:b2830
Kegg Gene:b2830
EchoBASE ID:EB2896
CCDB:RPPH_ECOLI
BacMap:16130734
General Reference:
  • Badger, J. L., Wass, C. A., Kim, K. S. (2000). "Identification of Escherichia coli K1 genes contributing to human brain microvascular endothelial cell invasion by differential fluorescence induction." Mol Microbiol 36:174-182. Pubmed: 10760174
  • Bessman, M. J., Walsh, J. D., Dunn, C. A., Swaminathan, J., Weldon, J. E., Shen, J. (2001). "The gene ygdP, associated with the invasiveness of Escherichia coli K1, designates a Nudix hydrolase, Orf176, active on adenosine (5')-pentaphospho-(5')-adenosine (Ap5A)." J Biol Chem 276:37834-37838. Pubmed: 11479323
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Deana, A., Celesnik, H., Belasco, J. G. (2008). "The bacterial enzyme RppH triggers messenger RNA degradation by 5' pyrophosphate removal." Nature 451:355-358. Pubmed: 18202662
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553