Identification
Name:30S ribosomal protein S13
Synonyms:Not Available
Gene Name:rpsM
Enzyme Class:Not Available
Biological Properties
General Function:translation
Specific Function:Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA.In the E.coli 70S ribosome in the initiation state (PubMed:12809609) was modeled to contact the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; bridge B1a is broken in the model with bound EF-G, while the protein-protein contacts between S13 and L5 in B1b change (PubMed:12809609). The 23S rRNA contact site in bridge B1a is modeled to differ in different ribosomal states (PubMed:16272117), contacting alternately S13 or S19. In the two 3.5 angstroms resolved ribosome structures (PubMed:12859903) the contacts between L5, S13 and S19 bridge B1b are different, confirming the dynamic nature of this interaction. Bridge B1a is not visible in the crystallized ribosomes due to 23S rRNA disorder.Contacts the tRNAs in the A and P sites.The C-terminal tail plays a role in the affinity of the 30S P site for different tRNAs.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
GO Classification:
Function
cytosolic small ribosomal subunit
rRNA binding
structural constituent of ribosome
translation
tRNA binding
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>357
gtggcccgtatagcaggcattaacattcctgatcataagcatgccgtaatcgcattaact
tcgatttatggcgtcggcaagacccgttctaaagccatcctggctgcagcgggtatcgct
gaagatgttaagatcagtgagctgtctgaaggacaaatcgacacgctgcgtgacgaagtt
gccaaatttgtcgttgaaggtgatctgcgccgtgaaatcagcatgagcatcaagcgcctg
atggatcttggttgctatcgcggtttgcgtcatcgtcgtggtctcccggttcgcggtcag
cgtaccaagaccaacgcacgtacccgtaagggtccgcgcaaaccgatcaagaaataa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:118
Protein Molecular Weight:13099
Protein Theoretical pI:Not Available
PDB File:1M5G
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>30S ribosomal protein S13
MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEV
AKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK
References
External Links:
ResourceLink
Uniprot ID:P0A7S9
Uniprot Name:RS13_ECOLI
PDB ID:1M5G
CCDB:RS13_ECOLI
General Reference:Not Available