Sulfurtransferase tusA (P0A890)

Identification
Name:Sulfurtransferase tusA
Synonyms:
  • tRNA 2-thiouridine synthesizing protein A
Gene Name:tusA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in sulfurtransferase activity
Specific Function:Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD. Seems to affect the stability of sigma-S, particularly during the logarithmic growth phase
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
protein binding
sulfurtransferase activity
transferase activity
transferase activity, transferring sulfur-containing groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:b3470
Gene OrientationCounterclockwise
Centisome Percentage:77.74
Left Sequence End3606774
Right Sequence End3607019
Gene Sequence:
>246 bp
ATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAA
AGTGGAACATTGCCATTGTTTGGCTATTCAGGATGGGGCAGACCAATGAAAAAAGCCCAC
ACAGGGGAGAGTGGGCTGAAATGGGAAGCTAAAGACTCAAGTAAACTTATCGGAAATAAG
GACCACGCATTACGGGGGCTATCATCGCCGATGGCCGTGATTCGGCAAATCAGGTTGATT
ACGTAG
Protein Properties
Pfam Domain Function:
Protein Residues:81
Protein Molecular Weight:9094
Protein Theoretical pI:5
PDB File:1DCJ
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Sulfurtransferase tusA
MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIADDPATTRDIPGFCTFME
HELVAKETDGLPYRYLIRKGG
References
External Links:
ResourceLink
Uniprot ID:P0A890
Uniprot Name:TUSA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674470
PDB ID:1DCJ
Ecogene ID:EG12216
Ecocyc:EG12216
ColiBase:b3470
Kegg Gene:b3470
EchoBASE ID:EB2130
CCDB:TUSA_ECOLI
BacMap:16131342
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ikeuchi, Y., Shigi, N., Kato, J., Nishimura, A., Suzuki, T. (2006). "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions." Mol Cell 21:97-108. Pubmed: 16387657
  • Ishii, Y., Yamada, H., Yamashino, T., Ohashi, K., Katoh, E., Shindo, H., Yamazaki, T., Mizuno, T. (2000). "Deletion of the yhhP gene results in filamentous cell morphology in Escherichia coli." Biosci Biotechnol Biochem 64:799-807. Pubmed: 10830496
  • Katoh, E., Hatta, T., Shindo, H., Ishii, Y., Yamada, H., Mizuno, T., Yamazaki, T. (2000). "High precision NMR structure of YhhP, a novel Escherichia coli protein implicated in cell division." J Mol Biol 304:219-229. Pubmed: 11080457
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Yamashino, T., Isomura, M., Ueguchi, C., Mizuno, T. (1998). "The yhhP gene encoding a small ubiquitous protein is fundamental for normal cell growth of Escherichia coli." J Bacteriol 180:2257-2261. Pubmed: 9555915