Leucyl/phenylalanyl-tRNA--protein transferase (P0A8P1)

Identification
Name:Leucyl/phenylalanyl-tRNA--protein transferase
Synonyms:
  • L/F-transferase
  • Leucyltransferase
  • Phenyalanyltransferase
Gene Name:aat
Enzyme Class:
Biological Properties
General Function:Posttranslational modification, protein turnover, chaperones
Specific Function:Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0L-Leucyl-tRNA+1.0Protein ↔ 1.0tRNA(Leu)+1.0L-Leucyl-protein
1.0L-Leucyl-tRNA + 1.0Protein ↔ 1.0tRNA(Leu) + 1.0L-Leucyl-protein
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Function
catalytic activity
leucyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring amino-acyl groups
Process
catabolic process
macromolecule catabolic process
metabolic process
protein catabolic process
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>705 bp
ATGCGCCTGGTTCAGCTTTCTCGCCATTCAATAGCCTTCCCTTCCCCGGAAGGCGCATTA
CGTGAGCCTAACGGCCTGCTGGCACTTGGGGGCGATCTTAGCCCTGCGCGCCTGTTAATG
GCTTACCAGCGTGGTATTTTTCCGTGGTTTTCTCCAGGCGACCCCATCCTCTGGTGGTCG
CCCGATCCCCGCGCGGTGCTATGGCCAGAATCACTGCATATCAGCCGTAGTATGAAGCGA
TTTCATAAACGCTCGCCCTATCGTGTCACGATGAATTACGCTTTTGGTCAGGTCATTGAA
GGCTGTGCCAGCGATCGCGAAGAAGGAACCTGGATCACGCGTGGCGTGGTCGAAGCCTAC
CATCGCCTTCACGAACTCGGCCATGCCCACTCCATTGAAGTCTGGCGTGAAGATGAGCTT
GTCGGCGGTATGTACGGCGTGGCCCAGGGAACGCTATTTTGTGGCGAGTCCATGTTCAGC
CGGATGGAAAATGCGTCTAAAACGGCGCTTCTGGTATTCTGTGAGGAATTTATCGGTCAT
GGCGGTAAGCTTATCGACTGCCAGGTCCTTAACGATCACACAGCATCGCTTGGTGCCTGC
GAAATTCCCCGCCGGGATTACCTTAATTATCTCAATCAAATGCGCCTCGGACGATTGCCG
AATAATTTCTGGGTACCACGATGCTTGTTTTCACCACAAGAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:234
Protein Molecular Weight:26618
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Leucyl/phenylalanyl-tRNA--protein transferase
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWS
PDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAY
HRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFSRMENASKTALLVFCEEFIGH
GGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE
References
External Links:
ResourceLink
Uniprot ID:P0A8P1
Uniprot Name:LFTR_ECOLI
GenBank Gene ID:M63145
Genebank Protein ID:146481
CCDB:LFTR_ECOLI
General Reference:
  • Abramochkin, G., Shrader, T. E. (1995). "The leucyl/phenylalanyl-tRNA-protein transferase. Overexpression and characterization of substrate recognition, domain structure, and secondary structure." J Biol Chem 270:20621-20628. Pubmed: 7657641
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cummings, H. S., Sands, J. F., Foreman, P. C., Fraser, J., Hershey, J. W. (1991). "Structure and expression of the infA operon encoding translational initiation factor IF1. Transcriptional control by growth rate." J Biol Chem 266:16491-16498. Pubmed: 1909328
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Shrader, T. E., Tobias, J. W., Varshavsky, A. (1993). "The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat." J Bacteriol 175:4364-4374. Pubmed: 8331068