ECMDB

Identification

Name:

Glutathione S-transferase

Synonyms:

Not Available

Gene Name:

gst

Enzyme Class:

2.5.1.18

Biological Properties

General Function:

Involved in protein binding

Specific Function:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles

Cellular Location:

Cytoplasm

SMPDB Pathways:

Glutathione metabolism PW000833
glutathione metabolism II PW001927
glutathione metabolism III PW002018

KEGG Pathways:

Drug metabolism - cytochrome P450 ec00982
Glutathione metabolism ec00480
Metabolism of xenobiotics by cytochrome P450 ec00980

KEGG Reactions:

1.0RX

1.0

↔

1.0Halide

1.0R-S-Glutathione

1.0RX + 1.0Glutathione ↔ 1.0Halide + 1.0R-S-Glutathione
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1.0

↔

1.0

1.01-Nitronaphthalene-7,8-oxide + 1.0Glutathione ↔ 1.01-Nitro-7-hydroxy-8-glutathionyl-7,8-dihydronaphthalene
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1.0

↔

1.0

1.01-Nitronaphthalene-7,8-oxide + 1.0Glutathione ↔ 1.01-Nitro-7-glutathionyl-8-hydroxy-7,8-dihydronaphthalene
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1.0

↔

1.0

1.01-Nitronaphthalene-5,6-oxide + 1.0Glutathione ↔ 1.01-Nitro-5-hydroxy-6-glutathionyl-5,6-dihydronaphthalene
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1.0

↔

1.0

1.01-Nitronaphthalene-5,6-oxide + 1.0Glutathione ↔ 1.01-Nitro-5-glutathionyl-6-hydroxy-5,6-dihydronaphthalene
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1.0

↔

1.0

1.0Bromobenzene-3,4-oxide + 1.0Glutathione ↔ 1.03,4-Dihydro-3-hydroxy-4-S-glutathionyl bromobenzene
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1.0

↔

1.0

1.0Bromobenzene-2,3-oxide + 1.0Glutathione ↔ 1.02,3-Dihydro-2-S-glutathionyl-3-hydroxy bromobenzene
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1.0

↔

1.0

1.0Benzo[a]pyrene-4,5-oxide + 1.0Glutathione ↔ 1.04,5-Dihydro-4-hydroxy-5-S-glutathionyl-benzo[a]pyrene
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1.0

↔

1.0

1.0Benzo[a]pyrene-7,8-diol + 1.0Glutathione ↔ 1.07,8-Dihydro-7-hydroxy-8-S-glutathionyl-benzo[a]pyrene + 1.0Water
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1.0

↔

1.0

1.02,2-Dichloroacetaldehyde + 1.0Glutathione ↔ 1.0S-(2,2-Dichloro-1-hydroxy)ethyl glutathione
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1.0

↔

1.0

1.0Hydrochloric acid

1.02,2-Dichloroacetaldehyde + 1.0Glutathione ↔ 1.0S-(2-Chloroacetyl)glutathione + 1.0Hydrochloric acid
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1.0

↔

1.0

1.0Hydrochloric acid

1.02-(S-Glutathionyl)acetyl chloride + 1.0Glutathione ↔ 1.02-(S-Glutathionyl)acetyl glutathione + 1.0Hydrochloric acid
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1.0Trichloroethene

1.0

↔

1.0

1.0Hydrochloric acid

1.0Trichloroethene + 1.0Glutathione ↔ 1.0S-(1,2-Dichlorovinyl)glutathione + 1.0Hydrochloric acid
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1.01,2-Dibromoethane

1.0

↔

1.0

2.0Hydrobromic acid

1.01,2-Dibromoethane + 1.0Glutathione + 1.0Hydrogen ion ↔ 1.0Glutathione episulfonium ion + 2.0Hydrobromic acid
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1.0Aflatoxin B1-exo-8,9-epoxide

1.0

↔

1.0Aflatoxin B1exo-8,9-epoxide-GSH

1.0Aflatoxin B1-exo-8,9-epoxide + 1.0Glutathione ↔ 1.0Aflatoxin B1exo-8,9-epoxide-GSH
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1.0RX

1.0

↔

1.0

1.0R-S-Glutathione

1.0RX + 1.0Glutathione ↔ 1.0Halide + 1.0R-S-Glutathione
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SMPDB Reactions:

1.0Naphthalene epoxide

1.0

→

1.0

1.0Naphthalene epoxide + 1.0Glutathione + 1.0(1R,2S)-Naphthalene 1,2-oxide → 1.0(1R)-Glutathionyl-(2R)-hydroxy-1,2-dihydronaphthalene
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1.0Naphthalene epoxide

1.0

→

1.0

1.0Naphthalene epoxide + 1.0Glutathione + 1.0(1R,2S)-Naphthalene 1,2-oxide → 1.0(1R)-Hydroxy-(2R)-glutathionyl-1,2-dihydronaphthalene
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1.0

1.0Naphthalene epoxide

1.0

→

1.0

1.0Glutathione + 1.0Naphthalene epoxide + 1.0(1R,2S)-Naphthalene 1,2-oxide → 1.0(1S)-Hydroxy-(2S)-glutathionyl-1,2-dihydronaphthalene
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1.0

→

1.0

1.0Glutathione + 1.01-Nitronaphthalene-5,6-oxide → 1.01-Nitro-5-glutathionyl-6-hydroxy-5,6-dihydronaphthalene
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1.0

→

1.0

1.01-Nitronaphthalene-5,6-oxide + 1.0Glutathione → 1.01-Nitro-5-hydroxy-6-glutathionyl-5,6-dihydronaphthalene
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1.0

→

1.0

1.0Glutathione + 1.01-Nitronaphthalene-7,8-oxide → 1.01-Nitro-7-glutathionyl-8-hydroxy-7,8-dihydronaphthalene
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1.0

→

1.0

1.01-Nitronaphthalene-7,8-oxide + 1.0Glutathione → 1.01-Nitro-7-hydroxy-8-glutathionyl-7,8-dihydronaphthalene
ReactionCard

1.0

→

1.0

1.0Glutathione + 1.02,2-Dichloroacetaldehyde → 1.0S-(Formylmethyl)glutathione
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1.0

→

1.0

1.0Glutathione + 1.0Bromobenzene-2,3-oxide → 1.02,3-Dihydro-2-S-glutathionyl-3-hydroxy bromobenzene
ReactionCard

1.0

→

1.0

1.0Hydrochloric acid

1.0Glutathione + 1.02-(S-Glutathionyl)acetyl chloride → 1.02-(S-Glutathionyl)acetyl glutathione + 1.0Hydrochloric acid
ReactionCard

1.0

←

1.0

1.0Bromobenzene-3,4-oxide + 1.0Glutathione ← 1.03,4-Dihydro-3-hydroxy-4-S-glutathionyl bromobenzene
ReactionCard

Complex Reactions:

1.0RX

1.0

→

1.0HX

1.0R-S-glutathione

1.0RX + 1.0Glutathione → 1.0HX + 1.0R-S-glutathione
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB20003	(1R)-Glutathionyl-(2R)-hydroxy-1,2-dihydronaphthalene	MetaboCard
ECMDB20004	(1R)-Hydroxy-(2R)-glutathionyl-1,2-dihydronaphthalene	MetaboCard
ECMDB20001	(1R,2S)-Naphthalene 1,2-oxide	MetaboCard
ECMDB20006	(1S)-Hydroxy-(2S)-glutathionyl-1,2-dihydronaphthalene	MetaboCard
ECMDB20024	1-Nitro-5-glutathionyl-6-hydroxy-5,6-dihydronaphthalene	MetaboCard
ECMDB20025	1-Nitro-5-hydroxy-6-glutathionyl-5,6-dihydronaphthalene	MetaboCard
ECMDB20026	1-Nitro-7-glutathionyl-8-hydroxy-7,8-dihydronaphthalene	MetaboCard
ECMDB20027	1-Nitro-7-hydroxy-8-glutathionyl-7,8-dihydronaphthalene	MetaboCard
ECMDB20028	1-Nitronaphthalene-5,6-oxide	MetaboCard
ECMDB20029	1-Nitronaphthalene-7,8-oxide	MetaboCard
ECMDB20053	2,2-Dichloroacetaldehyde	MetaboCard
ECMDB20055	2,3-Dihydro-2-S-glutathionyl-3-hydroxy bromobenzene	MetaboCard
ECMDB20034	2-(S-Glutathionyl)acetyl chloride	MetaboCard
ECMDB20035	2-(S-Glutathionyl)acetyl glutathione	MetaboCard
ECMDB20078	3,4-Dihydro-3-hydroxy-4-S-glutathionyl bromobenzene	MetaboCard
ECMDB20096	4,5-Dihydro-4-hydroxy-5-S-glutathionyl-benzo[a]pyrene	MetaboCard
ECMDB20088	4-Glutathionyl cyclophosphamide	MetaboCard
ECMDB20110	7,8-Dihydro-7-hydroxy-8-S-glutathionyl-benzo[a]pyrene	MetaboCard
ECMDB20118	Benzo[a]pyrene-4,5-oxide	MetaboCard
ECMDB20119	Benzo[a]pyrene-7,8-diol	MetaboCard
ECMDB20125	Bromobenzene-2,3-oxide	MetaboCard
ECMDB20126	Bromobenzene-3,4-oxide	MetaboCard
ECMDB00125	Glutathione	MetaboCard
ECMDB20154	Glutathione episulfonium ion	MetaboCard
ECMDB23737	Halide	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB20186	S-(1,2-Dichlorovinyl)glutathione	MetaboCard
ECMDB20188	S-(2,2-Dichloro-1-hydroxy)ethyl glutathione	MetaboCard
ECMDB20187	S-(2-Chloroacetyl)glutathione	MetaboCard
ECMDB20189	S-(Formylmethyl)glutathione	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b1635

Gene Orientation

Clockwise

Centisome Percentage:

36.91

Left Sequence End

1712401

Right Sequence End

1713006

Gene Sequence:

>606 bp
ATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGAT
AAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGATCGTCAGGGGAAA
ACGGCAATTACGCTGGCAAGTTTATATCAGCAATATGCTTGCGTTCAGGCATTAATTGAT
GCCGGAGCGGATATTAATAAGCAAGATCATACCTGTTTAAATCCTTTTTTAATTAGCTGT
CTGAACGATGATTTAACGCTACTACGAATTATTTTACCGGCTAAACCCGATCTTAATTGC
GTAACCCGTTTTGGCGGTGTCGGCCTGACGCCTGCCTGTGAAAAAGGCCATTTAAGTATT
GTAAAAGAGCTTTTGGCGCATACGGAGATTAACGTTAACCAGACCAACCATGTAGGCTGG
ACGCCGCTACTGGAAGCGATTGTGCTTAATGATGGTGGTATTAAACAGCAGGCGATTGTG
CAGTTATTGCTGGAACACGGTGCCAGCCCGCATCTGACCGATAAATATGGCAAAACGCCA
CTGGAACTGGCGCGGGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGT
GCATAA

Protein Properties

Pfam Domain Function:

GST_C (PF00043 )
GST_N (PF02798 )

Protein Residues:

201

Protein Molecular Weight:

22868

Protein Theoretical pI:

PDB File:

1N2A

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Glutathione S-transferase
MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYFAVNPKGQVPALLLDDG
TLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEE
YKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAA
FMQRMAERPEVQDALSAEGLK

References

External Links:

Resource	Link
Uniprot ID:	P0A9D2
Uniprot Name:	GST_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674461
PDB ID:	1N2A
Ecogene ID:	EG12613
Ecocyc:	EG12613
ColiBase:	b1635
Kegg Gene:	b1635
EchoBASE ID:	EB2497
CCDB:	GST_ECOLI
BacMap:	16129593

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Arca, P., Garcia, P., Hardisson, C., Suarez, J. E. (1990). "Purification and study of a bacterial glutathione S-transferase." FEBS Lett 263:77-79. Pubmed: 2185038
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Nishida, M., Harada, S., Noguchi, S., Satow, Y., Inoue, H., Takahashi, K. (1998). "Three-dimensional structure of Escherichia coli glutathione S-transferase complexed with glutathione sulfonate: catalytic roles of Cys10 and His106." J Mol Biol 281:135-147. Pubmed: 9680481
Nishida, M., Kong, K. H., Inoue, H., Takahashi, K. (1994). "Molecular cloning and site-directed mutagenesis of glutathione S-transferase from Escherichia coli. The conserved tyrosyl residue near the N terminus is not essential for catalysis." J Biol Chem 269:32536-32541. Pubmed: 7798255

Glutathione S-transferase (P0A9D2)