Ribosomal large subunit pseudouridine synthase A (P0AA37)

Identification
Name:Ribosomal large subunit pseudouridine synthase A
Synonyms:
  • rRNA pseudouridylate synthase A
  • rRNA-uridine isomerase A
Gene Name:rluA
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Responsible for synthesis of pseudouridine from uracil- 746 in 23S ribosomal RNA and from uracil-32 in the anticodon stem and loop of transfer RNAs
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0tRNA uridine(32) → 1.0tRNA pseudouridine(32)
1.0tRNA uridine(32) → 1.0tRNA pseudouridine(32)
ReactionCard
1.023S rRNA uridine(746) → 1.023S rRNA pseudouridine(746)
1.023S rRNA uridine(746) → 1.023S rRNA pseudouridine(746)
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Function
binding
catalytic activity
intramolecular transferase activity
isomerase activity
nucleic acid binding
pseudouridine synthase activity
RNA binding
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
pseudouridine synthesis
RNA metabolic process
RNA modification
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>660 bp
ATGGGGATGGAAAACTACAATCCACCGCAGGAACCCTGGTTGGTTATCCTGTATCAGGAT
GACCATATTATGGTGGTCAACAAGCCGAGCGGTTTGTTGTCAGTGCCGGGTCGTCTGGAA
GAGCACAAAGACAGCGTGATGACGCGCATTCAGCGTGATTATCCGCAGGCAGAATCGGTG
CATCGTCTGGATATGGCTACCAGCGGCGTGATTGTAGTGGCGCTGACCAAAGCCGCGGAG
CGGGAGTTAAAACGCCAGTTCCGCGAGCGCGAGCCGAAAAAGCAGTATGTGGCCCGCGTC
TGGGGGCATCCATCCCCCGCAGAAGGTCTGGTGGATCTGCCGCTGATTTGCGACTGGCCA
AACCGCCCGAAACAGAAAGTCTGTTACGAAACGGGTAAACCTGCGCAGACGGAATATGAA
GTGGTGGAGTATGCGGCGGATAACACGGCAAGAGTGGTGTTAAAACCGATTACCGGGCGT
TCGCATCAATTGCGTGTGCATATGCTGGCGCTGGGTCATCCGATTCTCGGCGATCGTTTT
TATGCATCACCAGAAGCGAGAGCGATGGCACCACGTTTGTTGCTACATGCAGAGATGTTG
ACGATTACCCATCCGGCGTATGGCAATAGTATGACGTTTAAAGCGCCAGCGGATTTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:219
Protein Molecular Weight:24861
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal large subunit pseudouridine synthase A
MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKDSVMTRIQRDYPQAESV
HRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGHPSPAEGLVDLPLICDWP
NRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGRSHQLRVHMLALGHPILGDRF
YASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPADF
References
External Links:
ResourceLink
Uniprot ID:P0AA37
Uniprot Name:RLUA_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786244
CCDB:RLUA_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Myler, P. J., Venkataraman, G. M., Lodes, M. J., Stuart, K. D. (1994). "A frequently amplified region in Leishmania contains a gene conserved in prokaryotes and eukaryotes." Gene 148:187-193. Pubmed: 7958944
  • Ramamurthy, V., Swann, S. L., Spedaliere, C. J., Mueller, E. G. (1999). "Role of cysteine residues in pseudouridine synthases of different families." Biochemistry 38:13106-13111. Pubmed: 10529181
  • Raychaudhuri, S., Niu, L., Conrad, J., Lane, B. G., Ofengand, J. (1999). "Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA." J Biol Chem 274:18880-18886. Pubmed: 10383384
  • Spedaliere, C. J., Hamilton, C. S., Mueller, E. G. (2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues." Biochemistry 39:9459-9465. Pubmed: 10924141
  • Wrzesinski, J., Nurse, K., Bakin, A., Lane, B. G., Ofengand, J. (1995). "A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe)." RNA 1:437-448. Pubmed: 7493321
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901