Cytochrome d ubiquinol oxidase subunit 1 (P0ABJ9)

Identification
Name:Cytochrome d ubiquinol oxidase subunit 1
Synonyms:
  • Cytochrome bd-I oxidase subunit I
  • Cytochrome d ubiquinol oxidase subunit I
Gene Name:cydA
Enzyme Class:Not Available
Biological Properties
General Function:Energy production and conversion
Specific Function:Cytochrome d terminal oxidase complex is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at low aeration
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • Oxidative phosphorylation PW000919
  • pyruvate to cytochrome bd terminal oxidase electron transfer PW002087
KEGG Pathways:
SMPDB Reactions:
2.0Thumb+1.0Thumb+4.0Thumb → 2.0Thumb+2.0Thumb+4.0Thumb
2.0Ubiquinol-1 + 1.0Oxygen + 4.0Hydrogen ion → 2.0Ubiquinone-1 + 2.0Water + 4.0Hydrogen ion
ReactionCard
1.0Thumb+4.0Thumb+1.0Electron → 2.0Thumb
1.0Oxygen + 4.0Hydrogen ion + 1.0Electron → 2.0Water
ReactionCard
Complex Reactions:
2.0Thumb+0.5Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+2.0Thumb
2.0Hydrogen ion + 0.5Oxygen + 1.0Ubiquinol-8 → 1.0Water + 1.0Ubiquinone-8 + 2.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Ubiquinol-8 + 1.0Oxygen → 1.0Ubiquinone-8 + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB21574Ubiquinol-1MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21438Ubiquinone-1MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b0733
Gene OrientationClockwise
Centisome Percentage:16.61
Left Sequence End770681
Right Sequence End772249
Gene Sequence:
>1569 bp
ATGTTAGATATAGTCGAACTGTCGCGCTTACAGTTTGCCTTGACCGCGATGTACCACTTC
CTTTTTGTGCCACTGACGCTCGGTATGGCGTTCCTGCTGGCCATTATGGAAACGGTCTAC
GTCCTCTCCGGCAAACAGATTTATAAAGATATGACCAAGTTCTGGGGCAAGTTGTTTGGT
ATCAACTTCGCTCTGGGTGTGGCTACCGGTCTGACCATGGAGTTCCAGTTCGGGACTAAC
TGGTCTTACTATTCCCACTATGTAGGGGATATCTTCGGTGCGCCGCTGGCAATCGAAGGT
CTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTCTTCGGTTGGGATCGT
CTGGGTAAAGTTCAGCATATGTGTGTCACCTGGCTGGTGGCGCTCGGTTCAAACCTGTCC
GCACTGTGGATTCTGGTTGCGAACGGCTGGATGCAAAACCCAATCGCGTCCGATTTCAAC
TTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCT
CAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTC
GGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTT
GCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAA
TCCGGCTACGAAATGGGCGACGTGCAGAAAACCAAACTGGCTGCTATTGAAGCCGAGTGG
GAAACGCAACCTGCGCCTGCTGCCTTTACTCTGTTCGGCATTCCTGATCAGGAAGAGGAG
ACGAACAAATTTGCGATTCAGATCCCTTACGCACTGGGCATCATTGCAACGCGTTCCGTG
GATACCCCGGTTATCGGCCTGAAAGAGCTGATGGTGCAGCATGAAGAACGCATTCGTAAC
GGGATGAAGGCGTACTCTCTGCTCGAACAACTGCGTTCTGGTTCTACCGACCAGGCGGTT
CGTGACCAGTTCAATAGCATGAAGAAAGACCTCGGTTACGGTCTGCTGCTGAAACGCTAT
ACGCCAAACGTGGCTGATGCGACTGAAGCGCAGATTCAACAGGCAACCAAAGACTCCATC
CCGCGTGTAGCGCCGCTGTACTTTGCGTTCCGTATCATGGTGGCGTGTGGCTTCCTGCTT
CTGGCAATCATCGCGCTCTCTTTCTGGAGTGTCATCCGCAACCGCATTGGCGAGAAAAAA
TGGCTTCTGCGCGCCGCGCTGTACGGTATTCCGCTGCCGTGGATTGCTGTAGAAGCGGGC
TGGTTCGTGGCTGAATATGGCCGCCAACCGTGGGCTATCGGTGAAGTGCTGCCGACAGCT
GTGGCGAACTCGTCACTGACCGCAGGCGATCTCATCTTCTCAATGGTGCTGATTTGCGGC
CTGTATACCCTGTTCCTGGTGGCAGAATTGTTCTTAATGTTCAAGTTTGCACGCCTCGGC
CCAAGCAGCCTGAAAACCGGTCGCTATCACTTTGAGCAGTCTTCCACGACTACTCAGCCG
GCACGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:522
Protein Molecular Weight:58205
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 23-42
  • 95-114
  • 130-149
  • 188-207
  • 220-239
  • 393-412
  • 471-490
Protein Sequence:
>Cytochrome d ubiquinol oxidase subunit 1
MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSGKQIYKDMTKFWGKLFG
INFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLMAFFLESTFVGLFFFGWDR
LGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFNFETMRMEMVSFSELVLNPVA
QVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKRSFAIAASFGMAAVLSVIVLGDE
SGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPDQEEETNKFAIQIPYALGIIATRSV
DTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSGSTDQAVRDQFNSMKKDLGYGLLLKRY
TPNVADATEAQIQQATKDSIPRVAPLYFAFRIMVACGFLLLAIIALSFWSVIRNRIGEKK
WLLRAALYGIPLPWIAVEAGWFVAEYGRQPWAIGEVLPTAVANSSLTAGDLIFSMVLICG
LYTLFLVAELFLMFKFARLGPSSLKTGRYHFEQSSTTTQPAR
References
External Links:
ResourceLink
Uniprot ID:P0ABJ9
Uniprot Name:CYDA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651327
Ecogene ID:EG10173
Ecocyc:EG10173
ColiBase:b0733
Kegg Gene:b0733
EchoBASE ID:EB0170
CCDB:CYDA_ECOLI
BacMap:90111166
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Dueweke, T. J., Gennis, R. B. (1990). "Epitopes of monoclonal antibodies which inhibit ubiquinol oxidase activity of Escherichia coli cytochrome d complex localize functional domain." J Biol Chem 265:4273-4277. Pubmed: 1689724
  • Georgiou, C. D., Dueweke, T. J., Gennis, R. B. (1988). "Beta-galactosidase gene fusions as probes for the cytoplasmic regions of subunits I and II of the membrane-bound cytochrome d terminal oxidase from Escherichia coli." J Biol Chem 263:13130-13137. Pubmed: 3138232
  • Green, G. N., Fang, H., Lin, R. J., Newton, G., Mather, M., Georgiou, C. D., Gennis, R. B. (1988). "The nucleotide sequence of the cyd locus encoding the two subunits of the cytochrome d terminal oxidase complex of Escherichia coli." J Biol Chem 263:13138-13143. Pubmed: 2843510
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Newton, G., Yun, C. H., Gennis, R. B. (1991). "Analysis of the topology of the cytochrome d terminal oxidase complex of Escherichia coli by alkaline phosphatase fusions." Mol Microbiol 5:2511-2518. Pubmed: 1724280
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137