Identification
Name:2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
Synonyms:Not Available
Gene Name:mhpB
Enzyme Class:
Biological Properties
General Function:Involved in iron ion binding
Specific Function:Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3- dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2- hydroxy-6-ketononatrienedioate, respectively
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Microbial metabolism in diverse environments ec01120
  • Phenylalanine metabolism ec00360
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.02-Hydroxy-3-(4-hydroxyphenyl)propenoic acid+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.02-Hydroxy-3-(4-hydroxyphenyl)propenoic acid + 1.0Oxygen + 1.02-Hydroxy-3-(4-hydroxyphenyl)propenoic acid → 1.0Hydrogen ion + 1.02-Hydroxy-6-ketononatrienedioate
ReactionCard
1.0Thumb+1.0Thumb1.0(2E,4Z)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate+1.0Thumb
1.03-(2,3-Dihydroxyphenyl)propionic acid + 1.0Oxygen → 1.0(2E,4Z)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB249152-Hydroxy-3-(4-hydroxyphenyl)propenoic acidMetaboCard
ECMDB040812-Hydroxy-6-ketononadienedicarboxylateMetaboCard
ECMDB200422-Hydroxy-6-ketononatrienedioateMetaboCard
ECMDB200643-(2,3-Dihydroxyphenyl)propanoateMetaboCard
ECMDB040613-(2,3-Dihydroxyphenyl)propionic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB20197Trans-2,3-DihydroxycinnamateMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ferrous iron binding
ion binding
iron ion binding
metal ion binding
oxidoreductase activity
transition metal ion binding
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b0348
Gene OrientationClockwise
Centisome Percentage:7.96
Left Sequence End369501
Right Sequence End370445
Gene Sequence:
>945 bp
ATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGC
ATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACT
CGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTG
AAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCT
CGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTG
CCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGC
CCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAAT
ATACATGTTATGTTCAAGTCTTCATTAAATCAGAACACTGAACATCAGCTGCGTTATCAG
GAAACGGAGTTTGTGATTAGTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCA
TTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCG
TTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCG
TCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGC
ATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCG
CGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTA
AAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGC
CATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:314
Protein Molecular Weight:34196
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYD
VMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQP
LEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGRFTSTLNKRVLFLGSGGLSHQ
PPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISAFGNWRSEGRYYRPIPE
WIAGFGSLSARTEN
References
External Links:
ResourceLink
Uniprot ID:P0ABR9
Uniprot Name:MHPB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674318
Ecogene ID:EG20274
Ecocyc:EG20274
ColiBase:b0348
Kegg Gene:b0348
EchoBASE ID:EB4167
CCDB:MHPB_ECOLI
BacMap:16128333
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bugg, T. D. (1993). "Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli." Biochim Biophys Acta 1202:258-264. Pubmed: 8399388
  • Ferrandez, A., Garcia, J. L., Diaz, E. (1997). "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12." J Bacteriol 179:2573-2581. Pubmed: 9098055
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Mendel, S., Arndt, A., Bugg, T. D. (2004). "Acid-base catalysis in the extradiol catechol dioxygenase reaction mechanism: site-directed mutagenesis of His-115 and His-179 in Escherichia coli 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (MhpB)." Biochemistry 43:13390-13396. Pubmed: 15491145
  • Spence, E. L., Kawamukai, M., Sanvoisin, J., Braven, H., Bugg, T. D. (1996). "Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): sequence analysis and biochemical properties of a third family of extradiol dioxygenases." J Bacteriol 178:5249-5256. Pubmed: 8752345