ECMDB: 3-deoxy-D-manno-octulosonic-acid transferase (P0AC75)

Identification

Name:

3-deoxy-D-manno-octulosonic-acid transferase

Synonyms:

KDO transferase

Gene Name:

waaA

Enzyme Class:

2.4.99.12

Biological Properties

General Function:

Involved in biosynthetic process

Specific Function:

Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA. Degraded by FtsH; therefore FtsH regulates the addition of the sugar moiety of the LPS and thus the maturation of the LPS precursor

Cellular Location:

Cell inner membrane; Single-pass membrane protein (Probable)

SMPDB Pathways:

Lipopolysaccharide biosynthesis PW000831
lipopolysaccharide biosynthesis II PW001905
lipopolysaccharide biosynthesis III PW002059

KEGG Pathways:

Lipopolysaccharide biosynthesis ec00540
Metabolic pathways eco01100

KEGG Reactions:

1.0

↔

1.0

1.02,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate + 1.0CMP-3-Deoxy-D-manno-octulosonate + 1.02,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate ↔ 1.0KDO-lipid IV(A) + 1.0Cytidine monophosphate
ReactionCard

1.0

↔

1.0Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A)

1.0

1.0KDO-lipid IV(A) + 1.0CMP-3-Deoxy-D-manno-octulosonate ↔ 1.0Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) + 1.0Cytidine monophosphate
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1.0Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A)

1.0

↔

1.0alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA

1.0

1.0Di[3-deoxy-D-manno-octulosonyl]-lipid IV(A) + 1.0CMP-3-Deoxy-D-manno-octulosonate ↔ 1.0alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + 1.0Cytidine monophosphate
ReactionCard

SMPDB Reactions:

1.0

→

1.0Cytidine monophosphate

1.0

1.0CMP-3-Deoxy-D-manno-octulosonate + 1.0(2-N,3-O-bis(3-Hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) → 1.0Cytidine monophosphate + 1.0Hydrogen ion + 1.0alpha-Kdo-(2→6)-lipid IVA + 1.0Cytidine monophosphate
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1.0

→

1.0Cytidine monophosphate

1.0

1.0a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA

1.0

1.0alpha-Kdo-(2→6)-lipid IVA + 1.0CMP-3-Deoxy-D-manno-octulosonate → 1.0Cytidine monophosphate + 1.0Hydrogen ion + 1.0a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA + 1.0Cytidine monophosphate + 1.0a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA
ReactionCard

EcoCyc Reactions:

1.0

1.0lipid IV_A

↔

1.0

1.0CMP-3-Deoxy-D-manno-octulosonate + 1.0lipid IV_A ↔ 1.0Hydrogen ion + 1.0KDO-lipid IV(A) + 1.0Cytidine monophosphate
ReactionCard

1.0

↔

1.0

1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IV_A

1.0

1.0KDO-lipid IV(A) + 1.0CMP-3-Deoxy-D-manno-octulosonate ↔ 1.0Hydrogen ion + 1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IV_A + 1.0Cytidine monophosphate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0CMP-3-Deoxy-D-manno-octulosonate + 1.02,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate → 1.0Cytidine monophosphate + 1.0Hydrogen ion + 1.0KDO-lipid IV(A)
ReactionCard

1.0

→

1.0

1.0CMP-3-Deoxy-D-manno-octulosonate + 1.0KDO-lipid IV(A) → 1.0Cytidine monophosphate + 1.0Hydrogen ion + 1.0KDO(2)-lipid IV(A)
ReactionCard

1.0

→

1.0alpha-Kdo-(2->6)-lipid IV(A)

1.0

1.02,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate + 1.0CMP-3-Deoxy-D-manno-octulosonate → 1.0alpha-Kdo-(2->6)-lipid IV(A) + 1.0Cytidine monophosphate
ReactionCard

1.0Alpha-Kdo-(2->6)-lipid IV(A)

1.0

→

1.0alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A)

1.0

1.0Alpha-Kdo-(2->6)-lipid IV(A) + 1.0CMP-3-Deoxy-D-manno-octulosonate → 1.0alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + 1.0Cytidine monophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB23091	(2-N,3-O-bis(3-Hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate)	MetaboCard
ECMDB20057	2,3,2'3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate	MetaboCard
ECMDB21136	2,3,2'3'-Tetrakis(beta-hydroxymyristoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1,4'-bisphosphate	MetaboCard
ECMDB24178	a-Kdo-(2->4)-a-Kdo-(2->6)-lipid IVA	MetaboCard
ECMDB24218	alpha-Kdo-(2→6)-lipid IVA	MetaboCard
ECMDB20133	CMP-3-Deoxy-D-manno-octulosonate	MetaboCard
ECMDB00095	Cytidine monophosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21235	KDO(2)-lipid IV(A)	MetaboCard
ECMDB21236	KDO-lipid IV(A)	MetaboCard

GO Classification:

Function
binding
carbohydrate binding
catalytic activity
sugar binding
transferase activity
Process
biosynthetic process
carbohydrate metabolic process
metabolic process
primary metabolic process

Gene Properties

Blattner:

b3633

Gene Orientation

Clockwise

Centisome Percentage:

82.04

Left Sequence End

3806563

Right Sequence End

3807840

Gene Sequence:

>1278 bp
ATGAAAACAACAGCAAAGCTGTCGTTCATGATGTTTGTTGAATGGTTTATCTGGGGCGCG
TGGTTTGTGCCATTGTGGTTGTGGTTAAGTAAAAGCGGTTTTAGTGCCGGAGAAATTGGC
TGGTCGTATGCCTGTACCGCCATTGCGGCGATCCTGTCGCCAATTCTGGTTGGCTCCATC
ACTGACCGCTTTTTCTCGGCGCAAAAAGTGCTGGCGGTATTGATGTTCGCAGGCGCGCTG
CTGATGTATTTCGCTGCGCAACAGACCACTTTTGCCGGGTTCTTCCCGTTACTGCTGGCC
TACTCGCTAACCTATATGCCGACCATTGCGCTGACTAACAGCATCGCTTTTGCCAACGTG
CCGGATGTTGAGCGTGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCC
TCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACT
AACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTC
CTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTG
GATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTCTTCTGTTCATTCCTG
TTTGCGATGCCACTAGCGTTCTATTACATCTTTGCCAACGGTTATCTGACCGAAGTTGGC
ATGAAAAACGCCACCGGCTGGATGACGCTCGGCCAGTTCTCTGAAATCTTCTTTATGCTG
GCATTGCCGTTTTTCACTAAACGCTTTGGTATCAAAAAGGTATTATTGCTTGGTCTGGTC
ACCGCTGCGATCCGCTATGGCTTCTTTATTTACGGTAGTGCGGATGAATATTTCACCTAC
GCGTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACC
GCTTACATCTATGTCGATAAAAAAGCCCCCGTGCATATGCGTACCGCTGCGCAGGGGCTG
ATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATG
ATGGAAAAGATGTTCGCTTATCAGGAACCGGTAAACGGACTGACTTTCAACTGGTCCGGG
ATGTGGACTTTCGGCGCGGTGATGATTGCCATTATCGCCGTGCTGTTCATGATTTTTTTC
CGCGAATCCGACAACGAAATTACGGCTATCAAGGTCGATGATCGCGATATTGCGTTGACA
CAAGGGGAAGTTAAATGA

Protein Properties

Pfam Domain Function:

Glycos_transf_1 (PF00534 )
Glycos_transf_N (PF04413 )

Protein Residues:

425

Protein Molecular Weight:

47291

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

1-20

Protein Sequence:

>3-deoxy-D-manno-octulosonic-acid transferase
MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGE
TLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNK
VDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYAKLGKFVRRLLRRITLI
AAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWIATST
HEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSGEVPSTS
TQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHTFNFKDICA
RLEQASGLITVTDATTLAKEVSSLLTDADYRSFYGRHAVEVLYQNQGALQRLLQLLEPYL
PPKTH

References

External Links:

Resource	Link
Uniprot ID:	P0AC75
Uniprot Name:	KDTA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1736823
Ecogene ID:	EG10520
Ecocyc:	EG10520
ColiBase:	b3633
Kegg Gene:	b3633
EchoBASE ID:	EB0515
CCDB:	KDTA_ECOLI
BacMap:	16131504

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Clementz, T. (1992). "The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli." J Bacteriol 174:7750-7756. Pubmed: 1447141
Clementz, T., Raetz, C. R. (1991). "A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing." J Biol Chem 266:9687-9696. Pubmed: 2033061
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Katz, C., Ron, E. Z. (2008). "Dual role of FtsH in regulating lipopolysaccharide biosynthesis in Escherichia coli." J Bacteriol 190:7117-7122. Pubmed: 18776015
Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620