Identification
Name:Acyl-CoA thioesterase I
Synonyms:
  • Lecithinase B
  • Lysophospholipase L1
  • Protease I
Gene Name:tesA
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.01-Acyl-sn-glycero-3-phosphocholine+1.0Thumb1.0Thumb+1.0Thumb
1.01-Acyl-sn-glycero-3-phosphocholine + 1.0Water ↔ 1.0Glycerophosphocholine + 1.0Carboxylate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0a palmitoleoyl-[acp] 1.0a holo-[acyl-carrier protein]+1.0Thumb+1.0Hexadecenoate (n-C16:1)
1.0Water + 1.0a palmitoleoyl-[acp]  → 1.0a holo-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0Hexadecenoate (n-C16:1)
ReactionCard
1.0 a cis-vaccenoyl-[acp]+1.0Thumb1.0Thumb+1.0a holo-[acyl-carrier protein]+1.0Thumb
1.0 a cis-vaccenoyl-[acp] + 1.0Water → 1.0Hydrogen ion + 1.0a holo-[acyl-carrier protein] + 1.0Vaccenic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB211131-Acyl-sn-glycero-3-phosphoethanolamine (N-C12:0)MetaboCard
ECMDB211141-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:0)MetaboCard
ECMDB211151-Acyl-sn-glycero-3-phosphoethanolamine (N-C14:1)MetaboCard
ECMDB211161-Acyl-sn-glycero-3-phosphoethanolamine (N-C16:0)MetaboCard
ECMDB211171-Acyl-sn-glycero-3-phosphoethanolamine (N-C16:1)MetaboCard
ECMDB211181-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:0)MetaboCard
ECMDB211191-Acyl-sn-glycero-3-phosphoethanolamine (N-C18:1)MetaboCard
ECMDB211201-Acyl-sn-glycero-3-phosphoglycerol (N-C12:0)MetaboCard
ECMDB211211-Acyl-sn-glycero-3-phosphoglycerol (N-C14:0)MetaboCard
ECMDB211221-Acyl-sn-glycero-3-phosphoglycerol (N-C14:1)MetaboCard
ECMDB211231-Acyl-sn-glycero-3-phosphoglycerol (N-C16:0)MetaboCard
ECMDB211241-Acyl-sn-glycero-3-phosphoglycerol (N-C16:1)MetaboCard
ECMDB211251-Acyl-sn-glycero-3-phosphoglycerol (N-C18:0)MetaboCard
ECMDB211261-Acyl-sn-glycero-3-phosphoglycerol (N-C18:1)MetaboCard
ECMDB211271-Dodecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211281-Hexadec-9-enoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211291-hexadecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211301-Octadec-11-enoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211311-Octadecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211321-Tetradec-7-enoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211331-Tetradecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB21209Dodecanoate (N-C12:0)MetaboCard
ECMDB00126Glycerol 3-phosphateMetaboCard
ECMDB00086GlycerophosphocholineMetaboCard
ECMDB21223GlycerophosphoglycerolMetaboCard
ECMDB00114GlycerylphosphorylethanolamineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21347Octadecanoate (N-C18:0)MetaboCard
ECMDB21348Octadecenoate (N-C18:1)MetaboCard
ECMDB00220Palmitic acidMetaboCard
ECMDB21361tetradecanoate (n-C14:0)MetaboCard
ECMDB21362Tetradecenoate (N-C14:1)MetaboCard
ECMDB21443Vaccenic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
lipase activity
Process
lipid metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b0494
Gene OrientationCounterclockwise
Centisome Percentage:11.17
Left Sequence End518363
Right Sequence End518989
Gene Sequence:
>627 bp
ATGATGAACTTCAACAATGTTTTCCGCTGGCATTTGCCCTTCCTGTTCCTGGTCCTGTTA
ACCTTCCGTGCCGCCGCAGCGGACACGTTATTGATTCTGGGTGATAGCCTGAGCGCCGGG
TATCGAATGTCTGCCAGCGCGGCCTGGCCTGCCTTGTTGAATGATAAGTGGCAGAGTAAA
ACGTCGGTAGTTAATGCCAGCATCAGCGGCGACACCTCGCAACAAGGACTGGCGCGCCTT
CCGGCTCTGCTGAAACAGCATCAGCCGCGTTGGGTGCTGGTTGAACTGGGCGGCAATGAC
GGTTTGCGTGGTTTTCAGCCACAGCAAACCGAGCAAACGCTGCGCCAGATTTTGCAGGAT
GTCAAAGCCGCCAACGCTGAACCATTGTTAATGCAAATACGTCTGCCTGCAAACTATGGT
CGCCGTTATAATGAAGCCTTTAGCGCCATTTACCCCAAACTCGCCAAAGAGTTTGATGTT
CCGCTGCTGCCCTTTTTTATGGAAGAGGTCTACCTCAAGCCACAATGGATGCAGGATGAC
GGTATTCATCCCAACCGCGACGCCCAGCCGTTTATTGCCGACTGGATGGCGAAGCAGTTG
CAGCCTTTAGTAAATCATGACTCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:208
Protein Molecular Weight:23622
Protein Theoretical pI:8
PDB File:1U8U
Signaling Regions:
  • 1-26
Transmembrane Regions:
  • None
Protein Sequence:
>Acyl-CoA thioesterase I
MMNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSASAAWPALLNDKWQSK
TSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLRGFQPQQTEQTLRQILQD
VKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVPLLPFFMEEVYLKPQWMQDD
GIHPNRDAQPFIADWMAKQLQPLVNHDS
References
External Links:
ResourceLink
Uniprot ID:P0ADA1
Uniprot Name:TESA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674633
PDB ID:1U8U
Ecogene ID:EG11542
Ecocyc:EG11542
ColiBase:b0494
Kegg Gene:b0494
EchoBASE ID:EB1504
CCDB:TESA_ECOLI
BacMap:16128478
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cho, H., Cronan, J. E. Jr (1993). "Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme." J Biol Chem 268:9238-9245. Pubmed: 8098033
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ichihara, S., Matsubara, Y., Kato, C., Akasaka, K., Mizushima, S. (1993). "Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants." J Bacteriol 175:1032-1037. Pubmed: 8432696
  • Karasawa, K., Kudo, I., Kobayashi, T., Homma, H., Chiba, N., Mizushima, H., Inoue, K., Nojima, S. (1991). "Lysophospholipase L1 from Escherichia coli K-12 overproducer." J Biochem 109:288-293. Pubmed: 1864840
  • Lee, L. C., Lee, Y. L., Leu, R. J., Shaw, J. F. (2006). "Functional role of catalytic triad and oxyanion hole-forming residues on enzyme activity of Escherichia coli thioesterase I/protease I/phospholipase L1." Biochem J 397:69-76. Pubmed: 16515533
  • Lo, Y. C., Lin, S. C., Shaw, J. F., Liaw, Y. C. (2003). "Crystal structure of Escherichia coli thioesterase I/protease I/lysophospholipase L1: consensus sequence blocks constitute the catalytic center of SGNH-hydrolases through a conserved hydrogen bond network." J Mol Biol 330:539-551. Pubmed: 12842470
  • Lo, Y. C., Lin, S. C., Shaw, J. F., Liaw, Y. C. (2005). "Substrate specificities of Escherichia coli thioesterase I/protease I/lysophospholipase L1 are governed by its switch loop movement." Biochemistry 44:1971-1979. Pubmed: 15697222