Lipopolysaccharide export system protein lptA (P0ADV1)

Identification
Name:Lipopolysaccharide export system protein lptA
Synonyms:Not Available
Gene Name:lptA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in lipopolysaccharide binding
Specific Function:Required for the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. May act as a chaperone that facilitates LPS transfer across the aquaeous environment of the periplasm. Interacts specifically with the lipid A domain of LPS
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
Transports:
Transport References:Not Available
Complex Reactions:
1.0Thumb+1.0Thumb+1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0(O16 antigen)x4 core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0(O16 antigen)x4 core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0(O16 antigen)x4 core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0(O16 antigen)x4 core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A) → 1.0Thumb+1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A)
1.0Adenosine triphosphate + 1.0Water + 1.0cold adapted KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0cold adapted KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A
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1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A diphosphate → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A diphosphate
1.0Adenosine triphosphate + 1.0Water + 1.0core oligosaccharide lipid A diphosphate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A diphosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO2-Lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.04-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.04-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO(2)-lipid IV(A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO(2)-lipid IV(A)
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Phosphoethanolamine KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Phosphoethanolamine KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0core oligosaccharide lipid A+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0core oligosaccharide lipid A + 1.0Water → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A) → 1.0Thumb+1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A)
1.0Adenosine triphosphate + 1.0Water + 1.0cold adapted KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0cold adapted KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO2-Lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO(2)-lipid IV(A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO(2)-lipid IV(A)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211734-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid AMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21235KDO(2)-lipid IV(A)MetaboCard
ECMDB21031KDO2-Lipid AMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB21352Phosphoethanolamine KDO(2)-lipid (A)MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
periplasmic space
Function
binding
lipopolysaccharide binding
Process
carbohydrate transport
establishment of localization
lipopolysaccharide transport
polysaccharide transport
transport
Gene Properties
Blattner:b3200
Gene OrientationClockwise
Centisome Percentage:72.02
Left Sequence End3341402
Right Sequence End3341959
Gene Sequence:
>558 bp
GTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTC
AAAACCCAAATCAGCAAAATACGCACGGGTCGTGCTTCTCCCAGCCTGCTGGATGGCATT
GTCGTGGAATATTACGGCACGCCGACGCCGCTGCGTCAGCTGGCAAGCGTAACGGTAGAA
GATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAA
GCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTT
CCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAA
GCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAA
GCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTA
CAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCA
GAACTGATGCAGTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:185
Protein Molecular Weight:20127
Protein Theoretical pI:10
Signaling Regions:
  • 1-27
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide export system protein lptA
MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQSLDMQGNVVTFTGNVIV
TQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNGKPVEGHASQMHYELAKDF
VVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRVTTVLVPSQLQDKNNKGQTPA
QKKGN
References
External Links:
ResourceLink
Uniprot ID:P0ADV1
Uniprot Name:LPTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21239027
Ecogene ID:EG12618
Ecocyc:EG12618
ColiBase:b3200
Kegg Gene:b3200
EchoBASE ID:EB2502
CCDB:LPTA_ECOLI
BacMap:16131090
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Powell, B. S., Court, D. L., Inada, T., Nakamura, Y., Michotey, V., Cui, X., Reizer, A., Saier, M. H. Jr, Reizer, J. (1995). "Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant." J Biol Chem 270:4822-4839. Pubmed: 7876255
  • Sperandeo, P., Cescutti, R., Villa, R., Di Benedetto, C., Candia, D., Deho, G., Polissi, A. (2007). "Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli." J Bacteriol 189:244-253. Pubmed: 17056748
  • Sperandeo, P., Lau, F. K., Carpentieri, A., De Castro, C., Molinaro, A., Deho, G., Silhavy, T. J., Polissi, A. (2008). "Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli." J Bacteriol 190:4460-4469. Pubmed: 18424520
  • Suits, M. D., Sperandeo, P., Deho, G., Polissi, A., Jia, Z. (2008). "Novel structure of the conserved gram-negative lipopolysaccharide transport protein A and mutagenesis analysis." J Mol Biol 380:476-488. Pubmed: 18534617
  • Tran, A. X., Trent, M. S., Whitfield, C. (2008). "The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway." J Biol Chem 283:20342-20349. Pubmed: 18480051