ECMDB: Antitoxin MazE (P0AE72)

Identification

Name:

Antitoxin MazE

Synonyms:

Not Available

Gene Name:

mazE

Enzyme Class:

Not Available

Biological Properties

General Function:

transcription, DNA-templated

Specific Function:

Antitoxin component of a type II toxin-antitoxin (TA) module. Labile antitoxin that binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity. Is considered to be an 'addiction' molecule as the cell dies in its absence. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. This effect can be rescued by expression of MazE, but after 6 hours in rich medium the overexpression of MazF leads to programmed cell death. Cell growth and viability are not affected when MazF and MazE are coexpressed. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their own transcription (PubMed:8650219). There are 3 operators to which MazE binds (PubMed:12533537). MazE has higher affinity for promoter DNA in the presence of MazF (PubMed:25564525).Cell death governed by the MazE-MazF and DinJ-YafQ TA modules seems to play a role in biofilm formation, while MazE-MazF is also implicated in cell death in liquid media.Might also serve to protect cells against bacteriophage; in the presence of MazE-MazF fewer P1 phage are produced than in a disrupted strain. For strain K38 most wild-type cells are killed but not by phage lysis; it was suggested that MazE-MazF causes P1 phage exclusion from the bacterial population. This phenomenon is strain dependent.

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Metabolites:

ECMDB ID	Name	View

GO Classification:

Function
DNA binding
regulation of transcription, DNA-templated
transcription, DNA-templated

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>249
atgatccacagtagcgtaaagcgttggggaaattcaccggcggtgcggatcccggctacg
ttaatgcaggcgctcaatctgaatattgatgatgaagtgaagattgacctggtggatggc
aaattaattattgagccagtgcgtaaagagcccgtatttacgcttgctgaactggtcaac
gacatcacgccggaaaacctccacgagaatatcgactggggagagccgaaagataaggaa
gtctggtaa

Protein Properties

Pfam Domain Function:

Not Available

Protein Residues:

Protein Molecular Weight:

9355

Protein Theoretical pI:

Not Available

PDB File:

1MVF

Signaling Regions:

Not Available

Transmembrane Regions:

Not Available

Protein Sequence:

>Antitoxin MazE
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVN
DITPENLHENIDWGEPKDKEVW

References

External Links:

Resource	Link
Uniprot ID:	P0AE72
Uniprot Name:	MAZE_ECOLI
PDB ID:	1MVF
CCDB:	MAZE_ECOLI

General Reference:

Not Available