ECMDB

Identification

Name:

Molybdopterin adenylyltransferase

Synonyms:

MPT adenylyltransferase

Gene Name:

mog

Enzyme Class:

2.7.7.75

Biological Properties

General Function:

Involved in Mo-molybdopterin cofactor biosynthetic process

Specific Function:

Catalyzes the adenylation of molybdopterin as part of the biosynthesis of the molybdenum-cofactor

Cellular Location:

Not Available

SMPDB Pathways:

GTP degradation PW001888

KEGG Pathways:

Sulfur relay system ec04122

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Molybdopterin ↔ 1.0Adenylated molybdopterin + 1.0Pyrophosphate
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Molybdopterin ↔ 1.0Pyrophosphate + 1.0Adenylated molybdopterin
ReactionCard

SMPDB Reactions:

1.0Molybdopterin

1.0

→

1.0Pyrophosphate

1.0Adenylyl-molybdopterin

1.0Molybdopterin + 1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Molybdopterin → 1.0Pyrophosphate + 1.0Adenylyl-molybdopterin + 1.0Adenylyl-molybdopterin
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Molybdopterin ↔ 1.0Adenylated molybdopterin + 1.0Pyrophosphate
ReactionCard

1.0

→

1.0

1.0Hydrogen ion + 1.0Molybdopterin + 1.0Adenosine triphosphate → 1.0Adenylated molybdopterin + 1.0Pyrophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB21307	Adenylated molybdopterin	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB02206	Molybdopterin	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard

GO Classification:

Process
cellular metabolic process
coenzyme biosynthetic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
Mo-molybdopterin cofactor biosynthetic process

Gene Properties

Blattner:

b0009

Gene Orientation

Clockwise

Centisome Percentage:

0.20

Left Sequence End

9306

Right Sequence End

9893

Gene Sequence:

>588 bp
ATGAATACTTTACGTATTGGCTTAGTTTCCATCTCTGATCGCGCATCCAGCGGCGTTTAT
CAGGATAAAGGCATCCCTGCGCTGGAAGAATGGCTGACATCGGCGCTAACCACGCCGTTT
GAACTGGAAACCCGCTTAATCCCCGATGAGCAGGCGATCATCGAGCAAACGTTGTGTGAG
CTGGTGGATGAAATGAGTTGCCATCTGGTGCTCACCACGGGCGGAACTGGCCCGGCGCGT
CGTGACGTAACGCCCGATGCGACGCTGGCAGTAGCGGACCGCGAGATGCCTGGCTTTGGT
GAACAGATGCGCCAGATCAGCCTGCATTTTGTACCAACTGCGATCCTTTCGCGTCAGGTG
GGCGTGATTCGCAAACAGGCGCTGATCCTTAACTTACCCGGTCAGCCGAAGTCTATTAAA
GAGACGCTGGAAGGTGTGAAGGACGCTGAGGGTAACGTTGTGGTACACGGTATTTTTGCC
AGCGTACCGTACTGCATTCAGTTGCTGGAAGGGCCATACGTTGAAACGGCACCGGAAGTG
GTTGCAGCATTCAGACCGAAGAGTGCAAGACGCGACGTTAGCGAATAA

Protein Properties

Pfam Domain Function:

MoCF_biosynth (PF00994 )

Protein Residues:

195

Protein Molecular Weight:

21222

Protein Theoretical pI:

PDB File:

1DI7

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Molybdopterin adenylyltransferase
MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELETRLIPDEQAIIEQTLCE
LVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQMRQISLHFVPTAILSRQV
GVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFASVPYCIQLLEGPYVETAPEV
VAAFRPKSARRDVSE

References

External Links:

Resource	Link
Uniprot ID:	P0AF03
Uniprot Name:	MOG_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674278
PDB ID:	1DI7
Ecogene ID:	EG11511
Ecocyc:	EG11511
ColiBase:	b0009
Kegg Gene:	b0009
EchoBASE ID:	EB1473
CCDB:	MOG_ECOLI
BacMap:	16128003

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
James, R., Dean, D. O., Debbage, J. (1993). "Five open reading frames upstream of the dnaK gene of E. coli." DNA Seq 3:327-332. Pubmed: 8400364
Liu, M. T., Wuebbens, M. M., Rajagopalan, K. V., Schindelin, H. (2000). "Crystal structure of the gephyrin-related molybdenum cofactor biosynthesis protein MogA from Escherichia coli." J Biol Chem 275:1814-1822. Pubmed: 10636880
Nichols, J. D., Rajagopalan, K. V. (2005). "In vitro molybdenum ligation to molybdopterin using purified components." J Biol Chem 280:7817-7822. Pubmed: 15632135
Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901

Molybdopterin adenylyltransferase (P0AF03)