Methylated-DNA--protein-cysteine methyltransferase (P0AFH0)

Identification
Name:Methylated-DNA--protein-cysteine methyltransferase
Synonyms:
  • 6-O-methylguanine-DNA methyltransferase
  • MGMT
  • O-6-methylguanine-DNA-alkyltransferase
Gene Name:ogt
Enzyme Class:
Biological Properties
General Function:Replication, recombination and repair
Specific Function:Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0DNA containing 6-O-methylguanine+1.0Protein L-cysteine ↔ 1.0DNA+1.0Protein S-methyl-L-cysteine
1.0DNA containing 6-O-methylguanine + 1.0Protein L-cysteine ↔ 1.0DNA + 1.0Protein S-methyl-L-cysteine
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Function
catalytic activity
methylated-DNA-[protein]-cysteine S-methyltransferase activity
methyltransferase activity
S-methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA repair
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>516 bp
ATGAGAAGAGTACTCTTTAGCTGTTTCTGCGGGCTACTGTGGAGTTCCAGTGGATGGGCA
GTTGACCCTTTAGGAACGATTAATATCAATTTGCACGGTAACGTTGTTGATTTCTCCTGT
ACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAA
CTACTGAACGCTGGCGATACCACGGCACTCGTCCCTTTTAGCCTGCGGCTGGAGGGATGT
CCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTG
GCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAATTACGTAATAGCGATCGC
TCCCGGCTCGCACTGGGGGAGGCGAGCCCGACTGAGGAAGTAGATGCAAATGGCAATGTC
ACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGGTGTGGCG
AAAGCGGATGCGATATTTATGATCAATTATAATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:171
Protein Molecular Weight:19179
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Methylated-DNA--protein-cysteine methyltransferase
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
GLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGA
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
References
External Links:
ResourceLink
Uniprot ID:P0AFH0
Uniprot Name:OGT_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786746
CCDB:OGT_ECOLI
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Harris, L. C., Potter, P. M., Margison, G. P. (1992). "Site directed mutagenesis of two cysteine residues in the E. coli ogt O6-alkylguanine DNA alkyltransferase protein." Biochem Biophys Res Commun 187:425-431. Pubmed: 1520330
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Potter, P. M., Wilkinson, M. C., Fitton, J., Carr, F. J., Brennand, J., Cooper, D. P., Margison, G. P. (1987). "Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli." Nucleic Acids Res 15:9177-9193. Pubmed: 2825131