Identification
Name:DNA base-flipping protein
Synonyms:Not Available
Gene Name:atl
Enzyme Class:Not Available
Biological Properties
General Function:DNA dealkylation involved in DNA repair
Specific Function:Involved in DNA damage recognition. Binds DNA containing O(6)-methylguanine and larger O(6)-alkylguanine adducts, and to double-stranded DNA that contains an AP (apurinic/apyrimidinic) site (PubMed:16027108, PubMed:18084297, PubMed:19269902, PubMed:20921378). Binds to the damaged base and flips the base out of the DNA duplex into an extrahelical conformation, which allows processing by repair proteins (PubMed:18084297). Works in partnership with the nucleotide excision repair (NER) pathway to enhance the repair of the O(6)-alkylguanine adducts larger than the methyl adduct (PubMed:19269902, PubMed:20921378). Also prevents methyl-directed mismatch repair (MMR)-mediated attack of the O(6)-alkylguanine:T mispairs for the larger alkyl groups (PubMed:20921378).
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
catalytic activity
damaged DNA binding
DNA dealkylation involved in DNA repair
enzyme binding
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>390
atgctggtttcttgcgcaatgcgacttcactcgggcgtttttccagattatgctgaaaaa
ttacctcaggaagaaaagatggaaaaagaagattcatttccccaacgcgtctggcaaatc
gtcgccgctattcccgaaggctatgtcaccacttacggtgatgtggcgaaactggcggga
tcgccccgcgccgcgcgccaggtgggcggtgtgttaaagcgtctccctgaaggcagcacc
ttaccctggcaccgggtggttaatcgccacggcacaatttcgctaaccggaccggattta
cagcgtcagcgacaggcattactggcagaaggtgtgatggtatcgggaagcgggcaaatc
gacttgcagcgttatcgctggaactactaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:129
Protein Molecular Weight:14449
Protein Theoretical pI:Not Available
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>DNA base-flipping protein
MLVSCAMRLHSGVFPDYAEKLPQEEKMEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAG
SPRAARQVGGVLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQI
DLQRYRWNY
References
External Links:
ResourceLink
Uniprot ID:P0AFP2
Uniprot Name:ATL_ECOLI
CCDB:ATL_ECOLI
General Reference:Not Available