Identification
Name:Negative modulator of initiation of replication
Synonyms:Not Available
Gene Name:seqA
Enzyme Class:Not Available
Biological Properties
General Function:regulation of transcription, DNA-templated
Specific Function:Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re-initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated. Can also bind to hemimethylated GATC sequences outside of oriC region. Binds, with less affinity, to fully methylated GATC sites and affects timing of replication. May play a role in chromosome organization and gene regulation.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
cytoplasm
DNA binding
identical protein binding
negative regulation of DNA-dependent DNA replication initiation
regulation of transcription, DNA-templated
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>546
atgaaaacgattgaagttgatgatgaactctacagctatattgccagccacactaagcat
atcggcgagagcgcatccgacattttacggcgtatgttgaaattttccgccgcatcacag
cctgctgctccggtgacgaaagaggttcgcgttgcgtcacctgctatcgtcgaagcgaag
ccggtcaaaacgattaaagacaaggttcgcgcaatgcgtgaacttctgctttcggatgaa
tacgcagagcaaaagcgagcggtcaatcgctttatgctgctgttgtctacactatattct
cttgacgcccaggcgtttgccgaagcaacggaatcgttgcacggtcgtacacgcgtttac
tttgcggcagatgaacaaacgctgctgaaaaatggtaatcagaccaagccgaaacatgtg
ccaggcacgccgtattgggtgatcaccaacaccaacaccggccgtaaatgcagcatgatc
gaacacatcatgcagtcgatgcaattcccggcggaattgattgagaaggtttgcggaact
atctaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:Not Available
Protein Molecular Weight:20315
Protein Theoretical pI:Not Available
PDB File:1IU3
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>Negative modulator of initiation of replication
MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAPVTKEVRVASPAIVEAK
PVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLDAQAFAEATESLHGRTRVY
FAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCGT
I
References
External Links:
ResourceLink
Uniprot ID:P0AFY8
Uniprot Name:SEQA_ECOLI
PDB ID:1IU3
CCDB:SEQA_ECOLI
General Reference:Not Available