| Identification |
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| Name: | 5-methylcytosine-specific restriction enzyme B |
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| Synonyms: | Not Available |
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| Gene Name: | mcrB |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | DNA restriction-modification system |
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| Specific Function: | Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Metabolites: | |
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| GO Classification: | | Function |
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| ATP binding | | ATPase activity | | DNA binding | | DNA catabolic process | | DNA restriction-modification system | | GTP binding | | GTPase activity | | restriction endodeoxyribonuclease activity |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >1380
atggaatctattcaaccctggattgaaaaatttattaagcaagcacagcaacaacgttcg
caatccactaaagattatccaacgtcttaccgtaacctgcgagtaaaattgagtttcggt
tatggtaattttacgtctattccctggtttgcatttcttggagaaggtcaggaagcttct
aacggtatatatcccgttattctctattataaagattttgatgagttggttttggcttat
ggtataagcgacacgaatgaaccacatgcccaatggcagttctcttcagacatacctaaa
acaatcgcagagtattttcaggcaacttcgggtgtatatcctaaaaaatacggacagtcc
tattacgcctgttcccaaaaagtctcacagggtattgattacacccgatttgcctctatg
ctggacaacataatcaacgactataaattaatatttaattctggcaagagtgttattcca
cctatgtcaaaaactgaatcatactgtctggaagatgcgttaaatgatttgtttatccct
gaaaccacaatagagacgatactcaaacgattaaccatcaaaaaaaatattatcctccag
gggccgcccggcgttggaaaaacctttgttgcacgccgtctggcttacttgctgacagga
gaaaaggctccgcaacgcgtcaatatggttcagttccatcaatcttatagctatgaggat
tttatacagggctatcgtccgaatggcgtcggcttccgacgtaaagacggcatattttac
aatttttgtcagcaagctaaagagcagccagagaaaaagtatatttttattatagatgaa
atcaatcgtgccaatctcagtaaagtatttggcgaagtgatgatgttaatggaacatgat
aaacgaggtgaaaactggtctgttcccctaacctactccgaaaacgatgaagaacgattc
tatgtcccggagaatgtttatatcatcggtttaatgaatactgccgatcgctctctggcc
gttgttgactatgccctacgcagacgattttctttcatagatattgagccaggttttgat
acaccacagttccggaattttttactgaataaaaaagcagaaccttcatttgttgagtct
ttatgccaaaaaatgaacgagttgaaccaggaaatcagcaaagaggccactatccttggg
aaaggattccgcattgggcatagttacttctgctgtgggttggaagatggcacctctccg
gatacgcaatggcttaatgaaattgtgatgacggatatcgcccctttactcgaagaatat
ttctttgatgacccctataaacaacagaaatggaccaacaaattattaggggactcatag
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 459 |
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| Protein Molecular Weight: | 53156 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 3SSC |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >5-methylcytosine-specific restriction enzyme B
MESIQPWIEKFIKQAQQQRSQSTKDYPTSYRNLRVKLSFGYGNFTSIPWFAFLGEGQEAS
NGIYPVILYYKDFDELVLAYGISDTNEPHAQWQFSSDIPKTIAEYFQATSGVYPKKYGQS
YYACSQKVSQGIDYTRFASMLDNIINDYKLIFNSGKSVIPPMSKTESYCLEDALNDLFIP
ETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYED
FIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYIFIIDEINRANLSKVFGEVMMLMEHD
KRGENWSVPLTYSENDEERFYVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFD
TPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILGKGFRIGHSYFCCGLEDGTSP
DTQWLNEIVMTDIAPLLEEYFFDDPYKQQKWTNKLLGDS |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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