3'(2'),5'-bisphosphate nucleotidase cysQ (P22255)

Identification
Name:3'(2'),5'-bisphosphate nucleotidase cysQ
Synonyms:
  • 3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
  • 3'-phosphoadenosine 5'-phosphate phosphatase
  • PAP phosphatase
  • DPNPase
Gene Name:cysQ
Enzyme Class:
Biological Properties
General Function:Involved in magnesium ion binding
Specific Function:Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May also convert adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS). Has 10000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2)
Cellular Location:Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoadenosine phosphosulfate + 1.0Water ↔ 1.0Adenosine phosphosulfate + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine 3',5'-diphosphate + 1.0Water ↔ 1.0Adenosine monophosphate + 1.0Phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.0Adenosine 3',5'-diphosphate → 1.0Adenosine monophosphate + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine 3',5'-diphosphate + 1.0Water → 1.0Adenosine monophosphate + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00061Adenosine 3',5'-diphosphateMetaboCard
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB01003Adenosine phosphosulfateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01134Phosphoadenosine phosphosulfateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on ester bonds
inositol or phosphatidylinositol phosphatase activity
ion binding
magnesium ion binding
metal ion binding
phosphatase activity
phosphoric ester hydrolase activity
Gene Properties
Blattner:b4214
Gene OrientationClockwise
Centisome Percentage:95.58
Left Sequence End4434778
Right Sequence End4435518
Gene Sequence:
>741 bp
ATGAGTATCCTTGTCACCCGCCCGTCTCCCGCTGGAGAAGAGTTAGTGAGCCGTCTGCGC
ACACTGGGGCAGGTGGCCTGGCATTTTCCGCTGATTGAGTTTTCTCCGGGTCAACAATTA
CCGCAACTTGCTGATCAACTGGCAGCGCTGGGGGAGAGCGATCTGTTGTTTGCCCTCTCG
CAACACGCGGTTGCTTTTGCCCAATCACAGCTGCATCAGCAAGATCGTAAATGGCCCCGA
CTACCTGATTATTTCGCCATTGGACGCACCACCGCACTGGCACTACATACCGTAAGTGGA
CAGAAGATTCTCTACCCGCAGGATCGGGAAATCAGCGAAGTCTTGCTACAATTACCTGAA
TTACAAAATATTGCGGGCAAACGTGCGCTGATATTACGTGGCAATGGTGGTCGTGAGCTA
ATTGGGGATACCCTGACGGCGCGCGGTGCTGAGGTCACTTTTTGTGAATGTTATCAACGA
TGCGCAATCCATTACGATGGTGCAGAAGAAGCGATGCGCTGGCAAGCCCGCGAGGTGACG
ATGGTCGTTGTTACCAGCGGTGAAATGTTGCAGCAACTCTGGTCGCTGATCCCACAATGG
TATCGTGAGCACTGGTTACTACACTGTCGACTATTGGTCGTCAGTGAGCGTTTGGCGAAA
CTCGCCCGGGAACTGGGCTGGCAAGACATTAAGGTCGCCGATAACGCTGACAACGATGCG
CTTTTACGGGCATTACAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:246
Protein Molecular Weight:27176
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3'(2'),5'-bisphosphate nucleotidase cysQ
MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAAHTVIMDGLRTLTPDVP
VLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNIALIDHGKPILGVVYAPVM
NVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHADAELKEYLQQLGEHQTTSIGS
SLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGAHVHDWQGKPLDYTPRESFLNPG
FRVSIY
References
External Links:
ResourceLink
Uniprot ID:P22255
Uniprot Name:CYSQ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676247
Ecogene ID:EG10043
Ecocyc:EG10043
ColiBase:b4214
Kegg Gene:b4214
EchoBASE ID:EB0041
CCDB:CYSQ_ECOLI
BacMap:16132036
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Fabiny, J. M., Jayakumar, A., Chinault, A. C., Barnes, E. M. Jr (1991). "Ammonium transport in Escherichia coli: localization and nucleotide sequence of the amtA gene." J Gen Microbiol 137:983-989. Pubmed: 1856684
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Liu, J., Beacham, I. R. (1990). "Transcription and regulation of the cpdB gene in Escherichia coli K12 and Salmonella typhimurium LT2: evidence for modulation of constitutive promoters by cyclic AMP-CRP complex." Mol Gen Genet 222:161-165. Pubmed: 2172762
  • Mechold, U., Ogryzko, V., Ngo, S., Danchin, A. (2006). "Oligoribonuclease is a common downstream target of lithium-induced pAp accumulation in Escherichia coli and human cells." Nucleic Acids Res 34:2364-2373. Pubmed: 16682444
  • Neuwald, A. F., Krishnan, B. R., Brikun, I., Kulakauskas, S., Suziedelis, K., Tomcsanyi, T., Leyh, T. S., Berg, D. E. (1992). "cysQ, a gene needed for cysteine synthesis in Escherichia coli K-12 only during aerobic growth." J Bacteriol 174:415-425. Pubmed: 1729235
  • Peng, Z., Verma, D. P. (1995). "A rice HAL2-like gene encodes a Ca(2+)-sensitive 3'(2'),5'-diphosphonucleoside 3'(2')-phosphohydrolase and complements yeast met22 and Escherichia coli cysQ mutations." J Biol Chem 270:29105-29110. Pubmed: 7493934
  • Spiegelberg, B. D., Xiong, J. P., Smith, J. J., Gu, R. F., York, J. D. (1999). "Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate." J Biol Chem 274:13619-13628. Pubmed: 10224133