tRNA (uracil-5-)-methyltransferase (P23003)

Identification
Name:tRNA (uracil-5-)-methyltransferase
Synonyms:
  • tRNA(M-5-U54)-methyltransferase
  • RUMT
Gene Name:trmA
Enzyme Class:
Biological Properties
General Function:Involved in tRNA (uracil-5-)-methyltransferase activity
Specific Function:Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
catalytic activity
methyltransferase activity
RNA methyltransferase activity
S-adenosylmethionine-dependent methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
tRNA (uracil-5-)-methyltransferase activity
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
RNA processing
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:b3965
Gene OrientationCounterclockwise
Centisome Percentage:89.67
Left Sequence End4160193
Right Sequence End4161293
Gene Sequence:
>1101 bp
ATGACCCCCGAACACCTTCCAACAGAACAGTATGAAGCGCAGTTAGCCGAAAAAGTGGTA
CGTTTGCAAAGTATGATGGCACCGTTTTCTGACCTGGTTCCGGAAGTGTTTCGCTCGCCG
GTCAGTCATTACCGGATGCGCGCGGAGTTCCGCATCTGGCACGATGGCGATGACCTGTAT
CACATCATTTTCGATCAACAAACCAAAAGCCGCATCCGCGTGGATAGCTTCCCCGCCGCC
AGTGAACTTATCAACCAGTTGATGACGGCGATGATTGCGGGTGTGCGTAATAATCCCGTT
CTGCGCCACAAGTTGTTCCAGATTGATTACCTCACTACACTGAGTAATCAGGCGGTGGTT
TCCCTGCTATACCATAAGAAGCTGGATGATGAGTGGCGTCAGGAAGCGGAGGCCCTGCGC
GATGCACTGCGCGCGCAGAATCTGAATGTGCATCTGATTGGTCGGGCAACGAAAACCAAA
ATCGAGCTGGATCAGGATTACATCGATGAACGTCTGCCGGTCGCAGGGAAAGAGATGATC
TACCGTCAGGTAGAAAACAGCTTTACCCAGCCGAACGCGGCGATGAATATTCAGATGCTG
GAATGGGCGCTGGACGTAACCAAAGGCTCAAAAGGCGATTTACTGGAGCTGTACTGCGGC
AACGGTAACTTTTCATTAGCGCTGGCGCGTAATTTTGATCGGGTATTAGCCACCGAAATC
GCTAAGCCGTCGGTTGCTGCTGCGCAATACAACATCGCAGCTAACCATATTGATAACGTA
CAAATTATTCGTATGGCGGCAGAAGAATTTACTCAGGCGATGAATGGTGTGCGCGAGTTT
AACCGCCTGCAAGGGATCGACTTAAAGAGTTATCAGTGCGAAACCATTTTTGTCGACCCT
CCGCGCAGCGGTCTGGACAGTGAAACCGAGAAAATGGTGCAGGCGTATCCGCGTATTTTG
TACATCTCCTGTAACCCGGAAACGTTATGCAAGAATCTGGAAACATTAAGCCAGACGCAC
AAGGTCGAACGTCTGGCTCTGTTTGATCAGTTCCCCTACACGCACCATATGGAGTGCGGC
GTATTACTGACCGCGAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:366
Protein Molecular Weight:41967
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA (uracil-5-)-methyltransferase
MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHYRMRAEFRIWHDGDDLY
HIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLRHKLFQIDYLTTLSNQAVV
SLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTKIELDQDYIDERLPVAGKEMI
YRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNGNFSLALARNFDRVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREFNRLQGIDLKSYQCETIFVDP
PRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQTHKVERLALFDQFPYTHHMECG
VLLTAK
References
External Links:
ResourceLink
Uniprot ID:P23003
Uniprot Name:TRMA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676096
Ecogene ID:EG11022
Ecocyc:EG11022
ColiBase:b3965
Kegg Gene:b3965
EchoBASE ID:EB1015
CCDB:TRMA_ECOLI
BacMap:16131803
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gustafsson, C., Lindstrom, P. H., Hagervall, T. G., Esberg, K. B., Bjork, G. R. (1991). "The trmA promoter has regulatory features and sequence elements in common with the rRNA P1 promoter family of Escherichia coli." J Bacteriol 173:1757-1764. Pubmed: 1999392
  • Gustafsson, C., Warne, S. R. (1992). "Physical map of the oxyR-trmA region (minute 89.3) of the Escherichia coli chromosome." J Bacteriol 174:7878-7879. Pubmed: 1447162
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lindstrom, P. H., Stuber, D., Bjork, G. R. (1985). "Genetic organization and transcription from the gene (trmA) responsible for synthesis of tRNA (uracil-5)-methyltransferase by Escherichia coli." J Bacteriol 164:1117-1123. Pubmed: 2999071
  • Ny, T., Bjork, G. R. (1980). "Cloning and restriction mapping of the trmA gene coding for transfer ribonucleic acid (5-methyluridine)-methyltransferase in Escherichia coli K-12." J Bacteriol 142:371-379. Pubmed: 6247318