5-keto-4-deoxy-D-glucarate aldolase (P23522)

Identification
Name:5-keto-4-deoxy-D-glucarate aldolase
Synonyms:
  • KDGluc aldolase
  • KDGlucA
  • 2-dehydro-3-deoxy-D-glucarate aldolase
  • 2-keto-3-deoxy-D-glucarate aldolase
  • 5-dehydro-4-deoxy-D-glucarate aldolase
  • Alpha-keto-beta-deoxy-D-glucarate aldolase
Gene Name:garL
Enzyme Class:
Biological Properties
General Function:Involved in carbon-carbon lyase activity
Specific Function:Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde
Cellular Location:Not Available
SMPDB Pathways:
  • superpathway of D-glucarate and D-galactarate degradation PW000795
KEGG Pathways:
KEGG Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.05-Dehydro-4-deoxy-D-glucarate → 1.0Tartronate semialdehyde + 1.0Pyruvic acid
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-Dehydro-4-deoxy-D-glucarate ↔ 1.0Pyruvic acid + 1.0Tartronate semialdehyde
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0Tartronate semialdehyde + 1.0Pyruvic acid ↔ 1.02-Dehydro-3-deoxy-D-glucarate
ReactionCard
EcoCyc Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.05-Dehydro-4-deoxy-D-glucarate → 1.0Tartronate semialdehyde + 1.0Pyruvic acid
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Dehydro-3-deoxy-D-glucarate → 1.0Pyruvic acid + 1.0Tartronate semialdehyde
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200402-Dehydro-3-deoxy-D-glucarateMetaboCard
ECMDB201025-Dehydro-4-deoxy-D-glucarateMetaboCard
ECMDB241965-dehydro-4-deoxy-D-glucarate(2−)MetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB06938Tartronate semialdehydeMetaboCard
GO Classification:
Function
carbon-carbon lyase activity
catalytic activity
lyase activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b3126
Gene OrientationCounterclockwise
Centisome Percentage:70.50
Left Sequence End3270809
Right Sequence End3271579
Gene Sequence:
>771 bp
ATGAAGATTATTACTTGCTATAAGTGCGTGCCTGATGAACAGGATATTGCGGTCAATAAT
GCTGATGGTTCATTAGACTTCAGCAAAGCCGATGCCAAAATAAGCCAATACGATCTCAAC
GCTATTGAAGCGGCTTGCCAGCTAAAGCAACAGGCAGCAGAGGCGCAGGTGACAGCCTTA
AGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGC
CCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACG
GCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGGCGAT
GGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATT
CCGGCAGTTAACGGCGTCAGCAAAATTATCTCCCTGACGGCAGATACCCTCACCGTTGAG
CGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTT
TCCACTGATATCAACTCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAA
AAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACGCAGAGGCAGCCTGGTCA
GAACAACAGGTTGCCGCGCCGAAACAGCGCGAACGTCAGCGCATCGTGATTGAAGGCGAC
GGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:256
Protein Molecular Weight:27384
Protein Theoretical pI:6
PDB File:1DXE
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>5-keto-4-deoxy-D-glucarate aldolase
MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWLVLDGEHAPNDISTFIP
QLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVETKEEAELAVASTRYPPEGI
RGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDNVDAIAATEGVDGIFVGPSDL
AAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAPVEADARRYLEWGATFVAVGSDL
GVFRSATQKLADTFKK
References
External Links:
ResourceLink
Uniprot ID:P23522
Uniprot Name:GARL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674296
PDB ID:1DXE
Ecogene ID:EG10016
Ecocyc:EG10016
ColiBase:b3126
Kegg Gene:b3126
EchoBASE ID:EB0016
CCDB:GARL_ECOLI
BacMap:16131018
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hubbard, B. K., Koch, M., Palmer, D. R., Babbitt, P. C., Gerlt, J. A. (1998). "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Biochemistry 37:14369-14375. Pubmed: 9772162
  • Izard, T., Blackwell, N. C. (2000). "Crystal structures of the metal-dependent 2-dehydro-3-deoxy-galactarate aldolase suggest a novel reaction mechanism." EMBO J 19:3849-3856. Pubmed: 10921867
  • Komine, Y., Inokuchi, H. (1991). "Precise mapping of the rnpB gene encoding the RNA component of RNase P in Escherichia coli K-12." J Bacteriol 173:1813-1816. Pubmed: 1705543
  • Monterrubio, R., Baldoma, L., Obradors, N., Aguilar, J., Badia, J. (2000). "A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." J Bacteriol 182:2672-2674. Pubmed: 10762278