Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (P25552)

Identification
Name:Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
Synonyms:
  • Guanosine pentaphosphate phosphohydrolase
  • pppGpp-5'-phosphohydrolase
Gene Name:gppA
Enzyme Class:
Biological Properties
General Function:Involved in guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity
Specific Function:Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Guanosine 3'-diphosphate 5'-triphosphate + 1.0Water ↔ 1.0Hydrogen ion + 1.0Phosphate + 1.0Guanosine 3',5'-bis(diphosphate)
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Guanosine 3'-diphosphate 5'-triphosphate + 1.0Water ↔ 1.0Guanosine 3',5'-bis(diphosphate) + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0Polyphosphate + 1.0Water ↔ 1.0Phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Guanosine 3'-diphosphate 5'-triphosphate + 1.0Water ↔ 1.0Hydrogen ion + 1.0Phosphate + 1.0Guanosine 3',5'-bis(diphosphate)
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanosine 3'-diphosphate 5'-triphosphate + 1.0Water → 1.0Guanosine 3',5'-bis(diphosphate) + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20158Guanosine 3',5'-bis(diphosphate)MetaboCard
ECMDB20156Guanosine 3'-diphosphate 5'-triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b3779
Gene OrientationCounterclockwise
Centisome Percentage:85.37
Left Sequence End3960768
Right Sequence End3962252
Gene Sequence:
>1485 bp
ATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGAATAGTTTTCATATG
CTGGTTGTGCGCGAGGTGGCTGGAAGCATCCAGACGCTGACGCGAATAAAACGCAAAGTG
CGTCTGGCTGCTGGCCTGAATAGCGAAAATGCCCTCTCCAATGAAGCAATGGAGCGCGGT
TGGCAATGTTTGCGCCTGTTTGCTGAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGC
GTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCG
CAGGAAATTCTCGGTTGTCCGGTACAGGTGATCAGCGGTGAAGAGGAAGCACGTCTGATT
TATCAGGGCGTTGCTCACACCACTGGTGGTGCCGATCAGCGCCTGGTGGTGGATATAGGC
GGAGCCAGTACTGAACTGGTAACCGGCACGGGTGCACAAACCACCTCGTTGTTCAGCCTG
TCGATGGGCTGCGTCACCTGGCTGGAACGCTATTTTGCCGATCGTAATCTGGGGCAGGAA
AATTTTGATGCTGCAGAAAAAGCGGCACGCGAAGTGTTACGTCCGGTTGCCGATGAATTA
CGGTATCACGGCTGGAAAGTGTGCGTTGGTGCTTCCGGCACCGTGCAGGCGTTACAGGAA
ATCATGATGGCACAGGGGATGGATGAACGCATTACCCTGGAAAAGTTGCAGCAATTGAAA
CAGCGAGCCATTCATTGCGGTCGGCTGGAGGAACTGGAGATTGACGGGCTGACGCTGGAA
CGTGCGTTAGTGTTCCCGAGTGGTCTGGCGATCCTGATCGCCATTTTTACCGAACTGAAT
ATTCAGTGTATGACCCTGGCGGGCGGTGCGCTGCGTGAAGGCCTGGTCTACGGCATGTTG
CATCTGGCCGTCGAGCAGGATATTCGCAGCCGTACGCTGCGTAATATTCAACGCCGCTTT
ATGATCGATATTGATCAGGCACAGCGCGTAGCCAAAGTTGCGGCTAACTTCTTCGATCAG
GTGGAAAACGAATGGCATCTTGAAGCAATAAGCCGCGATTTGCTCATCAGCGCCTGTCAG
CTTCATGAAATCGGCCTGAGCGTTGACTTCAAACAAGCGCCGCAACACGCTGCTTATCTG
GTGCGTAATCTGGATCTTCCCGGTTTTACCCCCGCACAGAAAAAATTGCTGGCGACGCTA
CTGCTCAACCAGACTAATCCGGTCGATCTCTCATCGCTGCATCAGCAAAATGCCGTACCG
CCGCGCGTCGCAGAACAACTCTGCCGTTTACTACGCCTGGCCATCATTTTTGCCAGCCGT
CGCCGTGACGATCTCGTGCCAGAGATGACATTACAGGCTAACCATGAACTGTTGACCTTG
ACGCTTCCGCAAGGTTGGCTAACCCAACATCCGCTGGGTAAAGAGATTATTGCTCAGGAA
AGCCAGTGGCAGAGCTATGTCCACTGGCCGCTGGAAGTGCATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:494
Protein Molecular Weight:54871
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAAGLNSENALSNEAMERG
WQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQEILGCPVQVISGEEEARLI
YQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSLSMGCVTWLERYFADRNLGQE
NFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQALQEIMMAQGMDERITLEKLQQLK
QRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIFTELNIQCMTLAGGALREGLVYGML
HLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVAANFFDQVENEWHLEAISRDLLISACQ
LHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPAQKKLLATLLLNQTNPVDLSSLHQQNAVP
PRVAEQLCRLLRLAIIFASRRRDDLVPEMTLQANHELLTLTLPQGWLTQHPLGKEIIAQE
SQWQSYVHWPLEVH
References
External Links:
ResourceLink
Uniprot ID:P25552
Uniprot Name:GPPA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676269
Ecogene ID:EG10413
Ecocyc:EG10413
ColiBase:b3779
Kegg Gene:b3779
EchoBASE ID:EB0408
CCDB:GPPA_ECOLI
BacMap:49176408
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kalman, M., Murphy, H., Cashel, M. (1991). "rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote." New Biol 3:886-895. Pubmed: 1931833
  • Keasling, J. D., Bertsch, L., Kornberg, A. (1993). "Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase." Proc Natl Acad Sci U S A 90:7029-7033. Pubmed: 8394006
  • Reizer, J., Reizer, A., Saier, M. H. Jr, Bork, P., Sander, C. (1993). "Exopolyphosphate phosphatase and guanosine pentaphosphate phosphatase belong to the sugar kinase/actin/hsp 70 superfamily." Trends Biochem Sci 18:247-248. Pubmed: 8212131
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293