DNA polymerase III subunit delta' (P28631)

Identification
Name:DNA polymerase III subunit delta'
Synonyms:Not Available
Gene Name:holB
Enzyme Class:
Biological Properties
General Function:Involved in DNA binding
Specific Function:DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dATP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dGTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dCTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0Thymidine 5'-triphosphate + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01532dATPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB01440dGTPMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Component
DNA polymerase complex
DNA polymerase III complex
macromolecular complex
protein complex
Function
3'-5' exonuclease activity
binding
catalytic activity
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
exonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleic acid binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b1099
Gene OrientationClockwise
Centisome Percentage:24.89
Left Sequence End1154985
Right Sequence End1155989
Gene Sequence:
>1005 bp
GTGAAACTGGATGAAATCGCTCGGCTGGCGGGAGTGTCGCGGACCACTGCAAGCTATGTT
ATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCT
GTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGC
ACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT
AACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAA
GATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCC
ATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGAC
CCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTT
GGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAG
ACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGT
TTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTAT
GAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAG
GCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGC
GACGGCAAACTGCCTTCTGACCTGGCAATTGCCACCTTTGGCGATAACGAACTGCTCGAC
TTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTG
CTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGC
ATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:334
Protein Molecular Weight:36936
Protein Theoretical pI:7
PDB File:1XXI
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase III subunit delta'
MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCG
HCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALL
TDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCRLHYLAPPPEQYAVTWLSREV
TMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQ
APARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQL
MSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
References
External Links:
ResourceLink
Uniprot ID:P28631
Uniprot Name:HOLB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321961
PDB ID:1XXI
Ecogene ID:EG11500
Ecocyc:EG11500
ColiBase:b1099
Kegg Gene:b1099
EchoBASE ID:EB1463
CCDB:HOLB_ECOLI
BacMap:16129062
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carter, J. R., Franden, M. A., Aebersold, R., McHenry, C. S. (1993). "Identification, isolation, and characterization of the structural gene encoding the delta' subunit of Escherichia coli DNA polymerase III holoenzyme." J Bacteriol 175:3812-3822. Pubmed: 8509334
  • Dong, Z., Onrust, R., Skangalis, M., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'." J Biol Chem 268:11758-11765. Pubmed: 8505303
  • Guenther, B., Onrust, R., Sali, A., O'Donnell, M., Kuriyan, J. (1997). "Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III." Cell 91:335-345. Pubmed: 9363942
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • O'Donnell, M. (1992). "Accessory protein function in the DNA polymerase III holoenzyme from E. coli." Bioessays 14:105-111. Pubmed: 1575709
  • Onrust, R., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. II. Characterization of delta and delta'." J Biol Chem 268:11766-11772. Pubmed: 8505304
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232