Glutamate-ammonia-ligase adenylyltransferase (P30870)

Identification
Name:Glutamate-ammonia-ligase adenylyltransferase
Synonyms:
  • Glutamine-synthetase adenylyltransferase
  • ATase
  • [Glutamate--ammonia-ligase] adenylyltransferase
Gene Name:glnE
Enzyme Class:
Biological Properties
General Function:Involved in [glutamate-ammonia-ligase] adenylyltransferase activity
Specific Function:Adenylation and deadenylation of glutamate--ammonia ligase
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Adenosine triphosphate ↔ 1.0Pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0[L-glutamate:ammonia ligase (ADP-forming)] → 1.0Thumb+1.0adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]
1.0Adenosine triphosphate + 1.0[L-glutamate:ammonia ligase (ADP-forming)] → 1.0Pyrophosphate + 1.0adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
adenylyltransferase activity
catalytic activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
[glutamate-ammonia-ligase] adenylyltransferase activity
Gene Properties
Blattner:b3053
Gene OrientationCounterclockwise
Centisome Percentage:68.86
Left Sequence End3194823
Right Sequence End3197663
Gene Sequence:
>2841 bp
ATGAAGCCGCTCTCTTCACCGTTACAGCAGTACTGGCAGACCGTTGTTGAGCGGCTGCCA
GAGCCTTTAGCCGAGGAATCACTTAGCGCACAGGCGAAGTCAGTACTTACTTTTAGTGAT
TTTGTGCAGGACAGCGTGATTGCGCATCCAGAGTGGCTGACGGAACTGGAAAGCCAACCG
CCGCAGGCCGACGAATGGCAGCATTACGCGGCATGGTTGCAGGAGGCGCTCTGTAATGTG
AGTGACGAAGCCGGGTTAATGCGCGAGCTGCGGCTATTCCGGCGGCGCATTATGGTGCGC
ATCGCCTGGGCGCAAACGCTGGCACTGGTTACTGAAGAGAGCATATTGCAGCAGCTCAGC
TATCTGGCGGAGACGCTGATTGTTGCGGCGCGTGACTGGCTGTATGACGCCTGCTGCCGC
GAGTGGGGAACGCCGTGCAATGCGCAGGGCGAAGCGCAACCGCTGCTGATTTTAGGCATG
GGTAAGCTGGGCGGTGGGGAGCTGAATTTCTCCTCTGATATCGATCTGATTTTTGCCTGG
CCGGAACATGGTTGTACGCAGGGTGGACGCCGGGAACTGGATAACGCGCAGTTTTTTACC
CGCATGGGGCAGCGGCTGATTAAAGTGCTGGATCAACCAACGCAGGATGGCTTCGTCTAT
CGCGTGGATATGCGGCTGCGTCCGTTTGGCGAAAGTGGCCCGCTGGTGCTGAGCTTTGCC
GCGTTGGAAGATTATTACCAGGAGCAGGGGCGCGACTGGGAGCGTTACGCGATGGTCAAG
GCGCGGATTATGGGCGATAGCGAAGGCGTCTATGCTAACGAGTTGCGTGCGATGCTGCGC
CCGTTTGTTTTCCGTCGTTACATCGATTTCAGCGTGATTCAGTCGCTGCGCAACATGAAA
GGGATGATTGCCCGTGAAGTGCGTCGACGTGGTTTGACCGACAATATCAAACTCGGCGCA
GGCGGCATTCGCGAAATTGAATTTATCGTTCAGGTGTTCCAGCTCATTCGCGGCGGACGC
GAACCGTCGCTGCAATCGCGCTCTTTACTGCCAACGCTCAGCGCCATTGCCGAGCTGCAT
CTGCTTTCTGAAAACGATGCTGAACAATTGCGAGTGGCGTATCTGTTCCTGCGGCGTCTG
GAAAACCTGCTGCAAAGCATTAACGACGAACAAACCCAGACGCTTCCTTCTGATGAGCTT
AATCGTGCGCGGCTGGCGTGGGCGATGGACTTTGCTGACTGGCCGCAACTGACCGGGGCG
CTGACCGCACATATGACCAATGTGCGCCGGGTGTTTAATGAATTGATTGGCGACGATGAA
AGTGAAACTCAGGAAGAGTCGCTGTCGGAACAGTGGCGTGAGCTGTGGCAGGATGCGTTG
CAGGAAGATGACACTACGCCAGTGCTGGCGCATCTTAGCGAGGATGATCGCAAACAGGTG
CTAACGCTGATTGCCGATTTCCGCAAAGAGCTGGATAAGCGCACCATCGGGCCGCGAGGA
CGTCAGGTGCTCGACCATCTGATGCCGCATCTGCTAAGTGATGTCTGTGCGCGTGAAGAC
GCTGCCGTTACGCTGTCGCGCATTACCGCCTTGCTGGTGGGGATTGTTACCCGCACCACC
TATTTAGAATTGCTCAGTGAATTCCCCGCGGCGCTTAAACATTTGATTTCTCTGTGTGCC
GCGTCGCCGATGATTGCCAGCCAGCTGGCGCGTTATCCATTATTGCTGGATGAATTGCTC
GATCCAAACACCCTTTACCAGCCGACGGCGACCGATGCCTACCGCGATGAGTTGCGCCAG
TATTTGCTGCGCGTGCCGGAAGATGACGAAGAGCAACAGCTTGAGGCGCTGCGTCAGTTC
AAACAGGCGCAGCTGTTACGCATCGCCGCAGCGGATATCGCCGGTACGCTACCGGTGATG
AAAGTGAGCGATCACTTAACCTGGCTGGCGGAAGCCATGATAGATGCCGTCGTTCAGCAG
GCGTGGGTTCAAATGGTTGCCCGCTACGGTAAGCCGAATCACCTGAACGAACGCGAAGGG
CGTGGTTTTGCGGTGGTCGGCTACGGCAAGCTGGGCGGCTGGGAGTTAGGCTACAGTTCC
GATCTTGACCTTATCTTCCTCCATGATTGCCCAATGGATGCGATGACTGACGGTGAGCGG
GAAATCGACGGGCGGCAGTTTTATCTGCGTCTGGCGCAACGCATTATGCATCTGTTCAGT
ACGCGTACCTCTTCCGGCATTTTGTATGAAGTGGATGCTCGACTGCGTCCGTCCGGGGCG
GCGGGAATGCTGGTGACATCCGCAGAAGCATTTGCCGATTATCAGAAAAACGAGGCCTGG
ACGTGGGAACATCAGGCGCTGGTGCGTGCGCGTGTAGTGTACGGCGATCCGCAGCTCACC
GCGCACTTTGACGCAGTGCGTCGCGAGATTATGACGCTGCCGCGTGAAGGTAAAACTCTG
CAAACGGAAGTGCGGGAAATGCGCGAGAAAATGCGCGCTCATCTCGGCAATAAACATCGC
GATCGCTTTGATATCAAAGCTGATGAAGGGGGAATTACCGATATCGAATTTATTACCCAA
TATCTGGTGTTGCGCTACGCTCATGAAAAACCGAAGTTAACGCGCTGGTCAGACAACGTG
CGTATTCTGGAACTACTGGCGCAAAACGACATTATGGAAGAGCAGGAAGCGATGGCGCTG
ACCCGTGCTTACACTACGCTTCGCGATGAACTTCATCATCTGGCATTACAGGAATTGCCG
GGCCATGTGTCGGAGGATTGCTTCACCGCAGAGCGTGAACTGGTGCGGGCAAGCTGGCAG
AAGTGGCTGGTGGAAGAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:946
Protein Molecular Weight:108417
Protein Theoretical pI:5
PDB File:1V4A
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glutamate-ammonia-ligase adenylyltransferase
MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQP
PQADEWQHYAAWLQEALCNVSDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILQQLS
YLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGMGKLGGGELNFSSDIDLIFAW
PEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMK
GMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLSAIAELH
LLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDELNRARLAWAMDFADWPQLTGA
LTAHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
LTLIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCAREDAAVTLSRITALLVGIVTRTT
YLELLSEFPAALKHLISLCAASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQ
YLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQ
AWVQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAW
TWEHQALVRARVVYGDPQLTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHR
DRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMAL
TRAYTTLRDELHHLALQELPGHVSEDCFTAERELVRASWQKWLVEE
References
External Links:
ResourceLink
Uniprot ID:P30870
Uniprot Name:GLNE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675854
PDB ID:1V4A
Ecogene ID:EG11602
Ecocyc:EG11602
ColiBase:b3053
Kegg Gene:b3053
EchoBASE ID:EB1559
CCDB:GLNE_ECOLI
BacMap:16130949
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • van Heeswijk, W. C., Rabenberg, M., Westerhoff, H. V., Kahn, D. (1993). "The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli." Mol Microbiol 9:443-457. Pubmed: 8412694
  • Xu, Y., Zhang, R., Joachimiak, A., Carr, P. D., Huber, T., Vasudevan, S. G., Ollis, D. L. (2004). "Structure of the N-terminal domain of Escherichia coli glutamine synthetase adenylyltransferase." Structure 12:861-869. Pubmed: 15130478