Identification
Name:Transcription-repair-coupling factor
Synonyms:Not Available
Gene Name:mfd
Enzyme Class:Not Available
Biological Properties
General Function:transcription-coupled nucleotide-excision repair, DNA damage recognition
Specific Function:Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site. Can also dissociate RNAP that is blocked by low concentration of nucleoside triphosphates or by physical obstruction, such as bound proteins. In addition, can rescue arrested complexes by promoting forward translocation. Has ATPase activity, which is required for removal of stalled RNAP, but seems to lack helicase activity. May act through a translocase activity that rewinds upstream DNA, leading either to translocation or to release of RNAP when the enzyme active site can not continue elongation.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
GO Classification:
Function
ATP binding
ATP-dependent helicase activity
cellular response to DNA damage stimulus
cytoplasm
damaged DNA binding
DNA binding
DNA repair
regulation of transcription, DNA-templated
transcription-coupled nucleotide-excision repair, DNA damage recognition
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>3447
atgcctgaacaatatcgttatacgctgcccgtcaaagcgggtgagcagcgtctgctgggc
gagttaaccggcgcagcctgtgcaacgctggtagcggaaattgccgaacgtcacgccggt
ccggtggtactcattgcaccagatatgcaaaatgctctgcgtttgcatgatgaaatcagc
cagtttaccgatcaaatggtgatgaatctggcggactgggaaactcttccctacgacagt
ttttcgcctcatcaggacattatctcctcgcgcctttccaccctttaccagctaccgacg
atgcagcgtggcgtactgattgttccggtgaatacgcttatgcagcgcgtttgcccacac
agttttctccacggtcatgcgctggtgatgaaaaaaggtcagcgcctgtcacgagatgca
ttacgaacccaactggacagcgccggttatcgccatgttgaccaggtgatggagcacggc
gaatacgccacgcgcggcgcgttgctggatctcttcccgatggggagtgagctgccttat
cgtcttgatttctttgatgatgaaatcgacagcctgcgggtgtttgacgtcgacagccag
cgcacgctggaggaagtagaagcgatcaatctgctgcccgcgcacgaatttccgaccgat
aaagcggcaattgaactgttccgcagccagtggcgcgataccttcgaagtgaagcgcgat
ccagaacatatttaccagcaagtgagtaaaggcacattacctgccgggatcgagtactgg
cagccattgttcttcagcgaaccactgccgccgctgttcagttatttccctgccaatacc
ttgctcgtgaatactggcgatctggaaaccagtgccgaacgtttccaggctgacacgctg
gcgcgttttgagaatcgcggcgtcgatccgatgcgcccgctgttgccaccacaatcgctc
tggctgcgggtggacgagctcttctcagagctgaaaaactggccccgggtgcagctaaaa
actgaacatttaccgacaaaagccgcgaatgccaatttaggtttccagaaactgccagac
ctggccgttcaggcacaacaaaaagcgccgctggatgcgctgcgtaagttcctcgagact
ttcgacggtccggtggtgttctcggtagaaagtgaaggtcgccgtgaagcgctgggtgaa
ctgctcgcacgaattaaaattgctccgcaacgcattatgcgtcttgatgaagccagcgac
cgtgggcgttatctgatgattggcgctgccgaacatggttttgtcgatacggtgcgtaat
ctggcgctgatttgcgaaagcgatctgctcggtgaacgcgttgcccgtcgtcgtcaggat
tctcgccgcaccatcaaccccgatacactgatccgtaaccttgcggaactgcatattggt
cagccggtggtccatctggagcacggcgtcggtcgttatgccggaatgaccacgctggaa
gcgggtggcattactggcgagtatctgatgctcacctatgccaacgacgccaaactgtat
gttccggtgtcgtcactgcatctgattagccgttacgcaggtggcgcggaagaaaacgcc
ccgctgcataaacttggcggcgatgcgtggtcacgcgcgcggcagaaagcggcggaaaaa
gtgcgtgatgtggcggcggaattgctggatatctacgcgcaacgcgccgccaaagagggc
ttcgcgtttaaacacgatcgtgagcagtatcagttgttctgcgacagcttcccgtttgaa
accacgccggatcaggcgcaggccattaatgcggtacttagcgacatgtgtcagccgctg
gcaatggatcgtctggtgtgcggcgatgttggctttggtaaaacagaagtggcgatgcgc
gcagctttcctggcagtagataaccacaagcaggtggcggtgctggtgcctaccaccctt
ctcgcgcagcagcattacgacaacttccgcgaccgtttcgccaactggccggtacgtatc
gaaatgatctcccgtttccgcagcgccaaagagcagacgcaaatccttgcggaagtggcg
gaagggaaaatcgatattctgatcggtacgcacaaactgctgcaaagtgacgtcaagttt
aaagatttaggcctgctgattgtcgatgaagaacaccgcttcggggtgcgtcataaagag
cgcattaaagcgatgcgcgcgaacgtggatattctgacgcttactgcaacgccgatccca
cgtacgctgaatatggcaatgagcggaatgcgtgacctgtcgattatcgccacgccgccc
gcccgtcgtctggcagttaaaacctttgtccgtgagtatgacagcatggtggtccgggag
gcgatcctgcgtgaaattttgcgcggaggacaggtttattatctctacaatgatgtggaa
aacattcagaaagccgccgaacggctggcagaactggtgccagaagcgcggatcgccatc
ggtcacgggcagatgcgcgagcgcgaactggaacgggtgatgaatgatttccatcatcaa
cgtttcaacgtgctggtttgtacaaccattatcgaaaccgggatcgacatcccgacagcc
aacactattatcattgaacgcgcggatcacttcggtctggcgcagctgcaccagttacgc
ggtcgcgtcggacgttcgcatcatcaggcatatgcatggttgctgacaccgcatccaaaa
gcgatgactaccgatgcacaaaaacgtcttgaagcaattgcctcgctggaagatctcggg
gcaggttttgcgctggcaacgcacgatctggagattcgcggcgcgggtgaactgcttggc
gaagaacaaagcggctcaatggaaaccatcggtttctcgctgtatatggagttgctggaa
aacgccgtcgatgcactgaaagccggacgcgagccgtcgctggaagatctcaccagccag
caaacagaagtcgagctgcggatgccgtcgctattgccagatgatttcatccctgacgtg
aacacgcgtctgtcgttctacaaacgtattgccagcgccaaaacggaaaacgaactggaa
gagatcaaagtcgagcttatcgatcgcttcggcctgctgccagatccggcgcgtaccctg
ctggatattgcccgtctgcgccagcaagcgcagaaactggggatcaggaagctggaaggt
aatgagaaaggcggggtgatcgaatttgccgagaagaatcacgttaatccggcctggttg
attggtttgctgcaaaaacagccgcagcattaccgccttgatggtccgacgcgcctgaaa
tttattcaggatttgagtgagcggaaaacgcgtatcgaatgggtacgccagtttatgcgt
gaactggaagagaacgcgatcgcttaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:1148
Protein Molecular Weight:129981
Protein Theoretical pI:Not Available
PDB File:2B2N
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>Transcription-repair-coupling factor
MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVLIAPDMQNALRLHDEIS
QFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQRGVLIVPVNTLMQRVCPH
SFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHGEYATRGALLDLFPMGSELPY
RLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFPTDKAAIELFRSQWRDTFEVKRD
PEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYFPANTLLVNTGDLETSAERFQADTL
ARFENRGVDPMRPLLPPQSLWLRVDELFSELKNWPRVQLKTEHLPTKAANANLGFQKLPD
LAVQAQQKAPLDALRKFLETFDGPVVFSVESEGRREALGELLARIKIAPQRIMRLDEASD
RGRYLMIGAAEHGFVDTVRNLALICESDLLGERVARRRQDSRRTINPDTLIRNLAELHIG
QPVVHLEHGVGRYAGMTTLEAGGITGEYLMLTYANDAKLYVPVSSLHLISRYAGGAEENA
PLHKLGGDAWSRARQKAAEKVRDVAAELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFE
TTPDQAQAINAVLSDMCQPLAMDRLVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTL
LAQQHYDNFRDRFANWPVRIEMISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKF
KDLGLLIVDEEHRFGVRHKERIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPP
ARRLAVKTFVREYDSMVVREAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAI
GHGQMRERELERVMNDFHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLR
GRVGRSHHQAYAWLLTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLG
EEQSGSMETIGFSLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDV
NTRLSFYKRIASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEG
NEKGGVIEFAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMR
ELEENAIA
References
External Links:
ResourceLink
Uniprot ID:P30958
Uniprot Name:MFD_ECOLI
PDB ID:2B2N
CCDB:MFD_ECOLI
General Reference:Not Available