L-rhamnose isomerase (P32170)

Identification
Name:L-rhamnose isomerase
Synonyms:Not Available
Gene Name:rhaA
Enzyme Class:
Biological Properties
General Function:Involved in L-rhamnose isomerase activity
Specific Function:L-rhamnose = L-rhamnulose
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
  • Fructose and mannose metabolism ec00051
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0alpha-L-Rhamnose + 1.0L-Rhamnose ↔ 1.0L-Rhamnulose
ReactionCard
1.0Thumb ↔ 1.0Thumb
1.0alpha-L-Rhamnose ↔ 1.0L-Rhamnulose
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0keto-L-rhamnulose+1.0keto-L-rhamnulose
1.0beta-L-Rhamnose → 1.0keto-L-rhamnulose + 1.0keto-L-rhamnulose
ReactionCard
1.0β-L-lyxopyranose ↔ 1.0Thumb
1.0β-L-lyxopyranose ↔ 1.0L-Threo-2-pentulose
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0L-Lyxose → 1.0L-Threo-2-pentulose
ReactionCard
1.0Thumb → 1.0Thumb
1.0alpha-L-Rhamnose → 1.0L-Rhamnulose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00849alpha-L-RhamnoseMetaboCard
ECMDB23078beta-L-RhamnoseMetaboCard
ECMDB04097L-LyxoseMetaboCard
ECMDB23077L-RhamnoseMetaboCard
ECMDB04144L-RhamnuloseMetaboCard
ECMDB00751L-Threo-2-pentuloseMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, interconverting aldoses and ketoses
ion binding
isomerase activity
L-rhamnose isomerase activity
manganese ion binding
metal ion binding
transition metal ion binding
Process
alcohol metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
rhamnose metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3903
Gene OrientationCounterclockwise
Centisome Percentage:88.21
Left Sequence End4092746
Right Sequence End4094005
Gene Sequence:
>1260 bp
GTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTA
TTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGC
GGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCCTCGCAGTTCTCATCGGTAAT
CTTCTCCTCGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACC
ACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTA
CTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTG
GGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACC
GTCACCGTCTTTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCT
ATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGAC
GCATTAAAAGCGGTCGTTCCCGCACAACCGTTAGATTTCAATGTCGCGCTGGCGCTGGTT
GTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAAT
GCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTT
ATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATT
GCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAAC
GATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAA
ACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAAT
TTTGTCGGCTGGTTGACCTTCCTTTCGGCAGCTATTCCTCCAGTGGGTGGCGTGATCATC
GCCGACTATCTGATGAACCGTCGCCGCTATGAGCACTTTGCGACCACGCGTATGATGAGT
GTCAATTGGGTGGCGATTCTGGCGGTCGCCTTGGGGATTGCTGCAGGCCACTGGTTACCG
GGAATTGTTCCGGTCAACGCGGTATTAGGTGGCGCGCTGAGCTATCTGATCCTTAACCCG
ATTTTGAATCGTAAAACGACAGCAGCAATGACGCATGTGGAGGCTAACAGTGTCGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:419
Protein Molecular Weight:47199
Protein Theoretical pI:6
PDB File:1DE6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>L-rhamnose isomerase
MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDDVSGFENPEGSLTGGIQ
ATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESDTPVSRDQIKPEHFKNWVE
WAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWIDHCKASRRVSAYFGEQLGTPS
VMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNPAHHIDAVESKLFGIGAESYTVG
SNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAAMLYVPQLLLHVSRPVRWDSDHVVL
LDDETQAIASEIVRHDLFDRVHIGLDFFDASINRIAAWVIGTRNMKKALLRALLEPTAEL
RKLEAAGDYTARLALLEEQKSLPWQAVWEMYCQRHDTPAGSEWLESVRAYEKEILSRRG
References
External Links:
ResourceLink
Uniprot ID:P32170
Uniprot Name:RHAA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674478
PDB ID:1DE6
Ecogene ID:EG11867
Ecocyc:EG11867
ColiBase:b3903
Kegg Gene:b3903
EchoBASE ID:EB1813
CCDB:RHAA_ECOLI
BacMap:49176441
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Egan, S. M., Schleif, R. F. (1993). "A regulatory cascade in the induction of rhaBAD." J Mol Biol 234:87-98. Pubmed: 8230210
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Holcroft, C. C., Egan, S. M. (2000). "Roles of cyclic AMP receptor protein and the carboxyl-terminal domain of the alpha subunit in transcription activation of the Escherichia coli rhaBAD operon." J Bacteriol 182:3529-3535. Pubmed: 10852886
  • Korndorfer, I. P., Fessner, W. D., Matthews, B. W. (2000). "The structure of rhamnose isomerase from Escherichia coli and its relation with xylose isomerase illustrates a change between inter and intra-subunit complementation during evolution." J Mol Biol 300:917-933. Pubmed: 10891278
  • Moralejo, P., Egan, S. M., Hidalgo, E., Aguilar, J. (1993). "Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli." J Bacteriol 175:5585-5594. Pubmed: 8396120
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293