Putative acyl-CoA dehydrogenase AidB (P33224)

Identification
Name:Putative acyl-CoA dehydrogenase AidB
Synonyms:Not Available
Gene Name:aidB
Enzyme Class:Not Available
Biological Properties
General Function:Not Available
Specific Function:Part of the adaptive DNA-repair response to alkylating agents. Could prevent alkylation damage by protecting DNA and destroying alkylating agents that have yet to reach their DNA target. Binds to double-stranded DNA with a preference for a DNA region that includes its own promoter. Shows weak isovaleryl-CoA dehydrogenase activity in vitro. Part of the adaptive DNA-repair response to alkylating agents. Could prevent alkylation damage by protecting DNA and destroying alkylating agents that have yet to reach their DNA target. Binds to double-stranded DNA with a preference for a DNA region that includes its own promoter. Shows weak isovaleryl-CoA dehydrogenase activity in vitro.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB01113Isovaleryl-CoAMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b4187
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>ENA|AAC77144|AAC77144.2 Escherichia coli str. K-12 substr. MG1655 isovaleryl CoA dehydrogenase
GTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTA
TACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATAGC
GATTTTCTAGCCAGTATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGG
CTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGAC
GATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTG
CACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGT
TTTATGTTACATGCGCAGGTTGAGGCAGGGTCGTTATGTCCGATAACCATGACCTTTGCC
GCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTA
TTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATT
GGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCA
GAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCG
CAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTTGTG
CCGCGCTTTTTGCCTGACGGGCAACGCAACGCGATTCGCCTCGAGCGGCTGAAAGATAAG
CTGGGTAATCGCTCTAACGCCAGTTGCGAAGTGGAGTTTCAGGATGCCATTGGTTGGTTG
TTGGGGCTGGAAGGGGAAGGAATTCGTCTGATCCTGAAAATGGGTGGGATGACGCGTTTT
GATTGCGCCCTGGGTAGCCATGCCATGATGCGCCGTGCATTTTCGCTGGCGATTTATCAT
GCACATCAACGCCATGTTTTTGGTAATCCATTGATCCAACAGCCCCTTATGCGTCATGTC
TTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGA
GCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCG
GCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTG
GGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTA
AACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAAT
AAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGAT
CGCTATTTTGATCGCGCGGTTCGTCGTTTACAGCAGCAGCTGCGTAAGCCAGCTGAAGAA
CTGGGGCGAGAGATTACTCATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAA
TATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGC
GTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGT
GTGTAA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:Not Available
Protein Molecular Weight:Not Available
Protein Theoretical pI:Not Available
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>sp|P33224|AIDB_ECOLI Putative acyl-CoA dehydrogenase AidB OS=Escherichia coli (strain K12) GN=aidB PE=1 SV=3
MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLASIGQQLGTAESLELGR
LANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHNLAWEEDARSGAFVARAAR
FMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPLLSDRYDSHLLPGGQKRGLLI
GMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFV
PRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGLEGEGIRLILKMGGMTRF
DCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLFRLAR
AWDRRADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRLYREMPV
NSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQLRKPAEE
LGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLLLRATGGVC
V
References
External Links:
ResourceLink
Uniprot ID:P33224
ColiBase:b4187
Kegg Gene:b4187
BacMap:162135915
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bowles, T., Metz, A. H., O'Quin, J., Wawrzak, Z., Eichman, B. F. (2008). "Structure and DNA binding of alkylation response protein AidB." Proc Natl Acad Sci U S A 105:15299-15304. Pubmed: 18829440
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Landini, P., Hajec, L. I., Volkert, M. R. (1994). "Structure and transcriptional regulation of the Escherichia coli adaptive response gene aidB." J Bacteriol 176:6583-6589. Pubmed: 7961409
  • Rippa, V., Amoresano, A., Esposito, C., Landini, P., Volkert, M., Duilio, A. (2010). "Specific DNA binding and regulation of its own expression by the AidB protein in Escherichia coli." J Bacteriol 192:6136-6142. Pubmed: 20889740
  • Rohankhedkar, M. S., Mulrooney, S. B., Wedemeyer, W. J., Hausinger, R. P. (2006). "The AidB component of the Escherichia coli adaptive response to alkylating agents is a flavin-containing, DNA-binding protein." J Bacteriol 188:223-230. Pubmed: 16352838