L-lactate permease (P33231)

Identification
Name:L-lactate permease
Synonyms:Not Available
Gene Name:lldP
Enzyme Class:Not Available
Biological Properties
General Function:Involved in lactate transmembrane transporter activity
Specific Function:Transports L-lactate across the membrane. Can also transport D-lactate and glycolate. Seems to be driven by a proton motive force
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB01311D-Lactic acidMetaboCard
ECMDB03035Glycolic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00190L-Lactic acidMetaboCard
GO Classification:
Function
carboxylic acid transmembrane transporter activity
lactate transmembrane transporter activity
monocarboxylic acid transmembrane transporter activity
organic acid transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
ion transport
lactate transport
organic anion transport
transport
Gene Properties
Blattner:b3603
Gene OrientationClockwise
Centisome Percentage:81.37
Left Sequence End3775422
Right Sequence End3777077
Gene Sequence:
>1656 bp
ATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTC
ATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATAC
GATCACCCAAACCAGTATCTGGTTAAACCGGCGACCACTATTGCCGACAATATGATGCCA
GTAATGCAGCATCCGGCGCAGGATAAAGAAACCCAGCAGAAGCTGGCAGAACTTGAGAAA
AAAACCGGTAAGAAACCGAATGTGGTTGTTTTCTTGCTGGACGATGTGGGCTGGATGGAC
GTCGGTTTTAACGGTGGCGGCGTGGCGGTGGGTAACCCTACACCAGATATCGACGCCGTT
GCCAGCCAGGGGCTGATTTTAACTTCGGCGTATTCTCAACCAAGCTCTTCCCCAACCCGC
GCCACCATTCTCACCGGACAATACTCCATCCACCACGGCATTCTGATGCCGCCAATGTAC
GGGCAACCGGGCGGGCTGCAAGGGTTAACCACGCTGCCGCAGTTGCTGCACGATCAGGGC
TACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAG
AACGTTGGCTTTGATGATTTCCGTGGCTTTAACTCGGTGTCTGATATGTACACCGAATGG
CGCGACGTTCACGTCAATCCGGAAGTGGCCCTGAGTCCGGACCGTTCTGAATACATCAAG
CAATTACCGTTCAGCAAAGATGACGTTCATGCGGTGCGCGGCGGCGAACAACAGGCCATT
GCCGACATTACGCCGAAATATATGGAAGATCTGGATCAACGCTGGATGGACTATGGCGTT
AAGTTCCTCGACAAGATGGCGAAGAGCGATAAACCATTCTTCCTCTACTACGGCACTCGT
GGCTGCCACTTCGATAACTACCCAAATGCGAAATATGCGGGTAGCTCTCCGGCACGCACC
TCGTATGGCGACTGCATGGTGGAGATGAACGATGTGTTCGCTAATCTGTATAAAACACTG
GAGAAAAACGGTCAGCTTGATAACACGCTGATCGTCTTTACCTCCGATAACGGACCGGAA
GCCGAAGTACCGCCGCACGGACGCACCCCGTTCCGTGGTGCGAAAGGTTCGACCTGGGAA
GGCGGCGTTCGCGTACCGACTTTCGTTTACTGGAAAGGGATGATCCAACCGCGTAAATCT
GACGGTATTGTCGATCTGGCAGATCTCTTCCCTACCGCGCTGGATCTGGCAGGGCATCCT
GGAGCGAAAGTGGCGAATTTAGTGCCGAAAACCACCTTTATCGATGGTGTGGACCAGACA
TCCTTCTTCCTGGGAACAAATGGTCAGTCTAACCGTAAGGCCGAGCACTACTTCCTCAAC
GGTAAACTCGCTGCTGTGCGTATGGATGAGTTCAAGTATCACGTCCTGATTCAGCAACCT
TACGCTTATACCCAGAGCGGATATCAGGGTGGATTCACCGGCACAGTAATGCAAACGGCG
GGATCGTCGGTGTTTAACCTCTACACCGATCCGCAGGAAAGCGACTCCATCGGCGTGCGC
CATATTCCGATGGGTGTACCGCTACAGACCGAAATGCACGCGTATATGGAGATCCTGAAA
AAATATCCACCACGCGCGCAGATTAAATCTGACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:551
Protein Molecular Weight:59168
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 13-33
  • 37-57
  • 70-90
  • 131-151
  • 159-179
  • 194-214
  • 245-265
  • 366-386
  • 405-425
  • 438-458
  • 494-514
  • 530-550
Protein Sequence:
>L-lactate permease
MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAASWTVAIALAVALLFYKM
PVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLI
VGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLCLIVNTAPVAFGAMGIPILVA
GQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWRGIKETWPAVVVAGGSFAIAQYL
SSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFRFGDLGASQVDMTLAHTGYTAGQVL
RAWTPFLFLTATVTLWSIPPFKALFASGGALYEWVINIPVPYLDKLVARMPPVVSEATAY
AAVFKFDWFSATGTAILFAALLSIVWLKMKPSDAISTFGSTLKELALPIYSIGMVLAFAF
ISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV
SDLLLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCIVGVITT
LQAYVLTWMIP
References
External Links:
ResourceLink
Uniprot ID:P33231
Uniprot Name:LLDP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676250
Ecogene ID:EG11961
Ecocyc:EG11961
ColiBase:b3603
Kegg Gene:b3603
EchoBASE ID:EB1904
CCDB:LLDP_ECOLI
BacMap:16131474
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Dong, J. M., Taylor, J. S., Latour, D. J., Iuchi, S., Lin, E. C. (1993). "Three overlapping lct genes involved in L-lactate utilization by Escherichia coli." J Bacteriol 175:6671-6678. Pubmed: 8407843
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nunez, M. F., Kwon, O., Wilson, T. H., Aguilar, J., Baldoma, L., Lin, E. C. (2002). "Transport of L-Lactate, D-Lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli." Biochem Biophys Res Commun 290:824-829. Pubmed: 11785976
  • Nunez, M. F., Pellicer, M. T., Badia, J., Aguilar, J., Baldoma, L. (2001). "The gene yghK linked to the glc operon of Escherichia coli encodes a permease for glycolate that is structurally and functionally similar to L-lactate permease." Microbiology 147:1069-1077. Pubmed: 11283302
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620