Nickel import ATP-binding protein NikE (P33594)

Identification
Name:Nickel import ATP-binding protein NikE
Synonyms:Not Available
Gene Name:nikE
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex NikABCDE involved in nickel import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Nickel → 1.0ADP + 1.0Hydrogen ion + 1.0Nickel + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Nickel → 1.0ADP + 1.0Inorganic phosphate + 1.0Nickel
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB21391NickelMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
plasma membrane
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
catalytic activity
cation binding
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
inorganic cation transmembrane transporter activity
ion binding
ion transmembrane transporter activity
metal ion binding
nickel ion binding
nickel ion transmembrane transporter activity
nickel-transporting ATPase activity
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
substrate-specific transmembrane transporter activity
transition metal ion binding
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
metal ion transport
nickel ion transport
transition metal ion transport
transport
Gene Properties
Blattner:b3480
Gene OrientationClockwise
Centisome Percentage:77.93
Left Sequence End3615799
Right Sequence End3616605
Gene Sequence:
>807 bp
ATGAAATTTGTCTCTTTTAATATCAACGGCCTGCGCGCCAGACCTCACCAGCTTGAAGCC
ATCGTCGAAAAGCACCAACCGGATGTGATTGGCCTGCAGGAGACAAAAGTTCATGACGAT
ATGTTTCCGCTCGAAGAGGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAA
GGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTT
CCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGCGGAAATCCCCTCACTGCTG
GGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATA
AAATTCCCGGCAAAAGCGCAGTTTTATCAGAATCTGCAAAACTACCTGGAAACCGAACTC
AAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGAT
ATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTG
CCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGC
CATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTT
GACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGT
TGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCC
CCCGTCTGGGCGACCTTCCGCCGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:268
Protein Molecular Weight:29721
Protein Theoretical pI:11
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Nickel import ATP-binding protein NikE
MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALLGRSGCGKSTLARLLVG
LESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAVNPRKTVREILREPMRHLL
SLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRVCLARALAVEPKLLILDEAVS
NLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERFCQRVMVMDNGQIVETQVVGEKL
TFSSDAGRVLQNAVLPAFPVRRRTTEKV
References
External Links:
ResourceLink
Uniprot ID:P33594
Uniprot Name:NIKE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742858
Ecogene ID:EG12079
Ecocyc:EG12079
ColiBase:b3480
Kegg Gene:b3480
EchoBASE ID:EB2004
CCDB:NIKE_ECOLI
BacMap:16131352
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Navarro, C., Wu, L. F., Mandrand-Berthelot, M. A. (1993). "The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel." Mol Microbiol 9:1181-1191. Pubmed: 7934931
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620