Identification
Name:Thermoresistant gluconokinase
Synonyms:
  • Gluconate kinase 2
Gene Name:gntK
Enzyme Class:
Biological Properties
General Function:Involved in shikimate kinase activity
Specific Function:ATP + D-gluconate = ADP + 6-phospho-D- gluconate
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB013166-Phosphogluconic acidMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00625Gluconic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
nucleoside binding
purine nucleoside binding
shikimate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3437
Gene OrientationCounterclockwise
Centisome Percentage:77.05
Left Sequence End3575088
Right Sequence End3575615
Gene Sequence:
>528 bp
TTGAGCACGACTAACCATGATCACCACATTTACGTCTTGATGGGCGTATCGGGCAGCGGC
AAATCTGCGGTCGCCAGTGAAGTGGCGCATCAACTTCATGCCGCGTTTCTTGATGGCGAT
TTCCTCCATCCACGGCGCAATATCGAAAAAATGGCGTCTGGCGAACCACTGAATGACGAC
GATCGCAAACCGTGGTTGCAGGCGCTGAACGACGCCGCGTTTGCTATGCAGCGCACTAAT
AAAGTGTCGCTGATCGTCTGTTCTGCATTGAAAAAACACTATCGCGACTTGCTGCGTGAA
GGTAATCCGAATCTCTCTTTCATCTATTTGAAAGGCGATTTTGATGTGATTGAAAGCCGC
CTGAAAGCGCGCAAAGGCCATTTCTTTAAAACCCAAATGTTGGTGACGCAGTTTGAAACG
CTGCAGGAGCCGGGTGCGGACGAAACCGATGTACTGGTGGTGGATATCGATCAACCGCTG
GAAGGTGTTGTGGCAAGCACCATTGAGGTTATTAAAAAAGGCAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:175
Protein Molecular Weight:19543
Protein Theoretical pI:7
PDB File:1KOF
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Thermoresistant gluconokinase
MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHPRRNIEKMASGEPLNDD
DRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGNPNLSFIYLKGDFDVIESR
LKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPLEGVVASTIEVIKKGK
References
External Links:
ResourceLink
Uniprot ID:P46859
Uniprot Name:GNTK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676606
PDB ID:1KOF
Ecogene ID:EG12629
Ecocyc:EG12629
ColiBase:b3437
Kegg Gene:b3437
EchoBASE ID:EB2513
CCDB:GNTK_ECOLI
BacMap:90111591
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Izu, H., Adachi, O., Yamada, M. (1997). "Gene organization and transcriptional regulation of the gntRKU operon involved in gluconate uptake and catabolism of Escherichia coli." J Mol Biol 267:778-793. Pubmed: 9135111
  • Kraft, L., Sprenger, G. A., Lindqvist, Y. (2001). "Crystallization and preliminary X-ray crystallographic studies of recombinant thermoresistant gluconate kinase GntK from Escherichia coli." Acta Crystallogr D Biol Crystallogr 57:1159-1161. Pubmed: 11468405
  • Tong, S., Porco, A., Isturiz, T., Conway, T. (1996). "Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism." J Bacteriol 178:3260-3269. Pubmed: 8655507