Octanoyltransferase (P60720)

Identification
Name:Octanoyltransferase
Synonyms:
  • Lipoate-protein ligase B
  • Lipoyl/octanoyl transferase
  • Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase
Gene Name:lipB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Octanoyl-[acp]+1.0Apoprotein+1.0Octanoyl-[acp]+1.0Protein ↔ 1.0Protein N6-(octanoyl)lysine+1.0Acyl-carrier protein+1.0Protein N6-(octanoyl)lysine+1.0Acyl-carrier protein
1.0Octanoyl-[acp] + 1.0Apoprotein + 1.0Octanoyl-[acp] + 1.0Protein ↔ 1.0Protein N6-(octanoyl)lysine + 1.0Acyl-carrier protein + 1.0Protein N6-(octanoyl)lysine + 1.0Acyl-carrier protein
ReactionCard
1.0Lipoyl-[acp]+1.0Apoprotein ↔ 1.0Protein N6-(lipoyl)lysine+1.0Acyl-carrier protein
1.0Lipoyl-[acp] + 1.0Apoprotein ↔ 1.0Protein N6-(lipoyl)lysine + 1.0Acyl-carrier protein
ReactionCard
SMPDB Reactions:
1.0Octanoyl-[acyl-carrier protein] +1.0apoprotein → 1.0[acyl carrier protein]+1.0Protein N6-(octanoyl)lysine
1.0Octanoyl-[acyl-carrier protein] + 1.0apoprotein → 1.0[acyl carrier protein] + 1.0Protein N6-(octanoyl)lysine
ReactionCard
1.0Lipoyl-ACP+1.0apoprotein → 1.0Protein N6-(lipoyl)lysine+1.0[ACP]
1.0Lipoyl-ACP + 1.0apoprotein → 1.0Protein N6-(lipoyl)lysine + 1.0[ACP]
ReactionCard
1.0Octanoyl-[acyl-carrier protein] +1.0a [lipoyl-carrier protein]-L-lysine → 1.0Thumb+1.0a holo-[acyl-carrier protein]+1.0Protein N6-(octanoyl)lysine
1.0Octanoyl-[acyl-carrier protein] + 1.0a [lipoyl-carrier protein]-L-lysine → 1.0Hydrogen ion + 1.0a holo-[acyl-carrier protein] + 1.0Protein N6-(octanoyl)lysine
ReactionCard
1.0an acetyl-[acp]+1.0a [lipoyl-carrier protein]-L-lysine → 1.0a [lipoyl-carrier protein] N6-octanoyl-L-lysine+1.0Thumb+1.0a holo-[acyl-carrier protein]
1.0an acetyl-[acp] + 1.0a [lipoyl-carrier protein]-L-lysine → 1.0a [lipoyl-carrier protein] N6-octanoyl-L-lysine + 1.0Hydrogen ion + 1.0a holo-[acyl-carrier protein]
ReactionCard
Complex Reactions:
1.0Thumb+1.0Octanoyl-ACP (n-C8:0ACP) → 1.0acyl carrier protein+1.0octanoate (protein bound)
1.0Hydrogen ion + 1.0Octanoyl-ACP (n-C8:0ACP) → 1.0acyl carrier protein + 1.0octanoate (protein bound)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acyltransferase activity
catalytic activity
octanoyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
lipoate biosynthetic process
lipoic acid biosynthetic process
lipoic acid metabolic process
macromolecule metabolic process
macromolecule modification
metabolic process
protein modification process
Gene Properties
Blattner:b0630
Gene OrientationCounterclockwise
Centisome Percentage:14.24
Left Sequence End660860
Right Sequence End661501
Gene Sequence:
>642 bp
TTGTATCAGGATAAAATTCTTGTCCGCCAGCTCGGTCTTCAGCCTTACGAGCCAATCTCC
CAGGCTATGCATGAATTCACCGATACCCGCGATGATAGTACCCTTGATGAAATCTGGCTG
GTCGAGCACTATCCGGTATTCACCCAAGGTCAGGCAGGAAAAGCGGAGCACATTTTAATG
CCGGGTGATATTCCGGTGATCCAGAGCGATCGCGGTGGGCAGGTGACTTATCACGGGCCG
GGGCAACAGGTGATGTATGTGTTGCTTAACCTGAAACGCCGTAAACTCGGTGTGCGTGAA
CTGGTGACCTTGCTTGAGCAAACAGTGGTGAATACCCTGGCTGAACTGGGTATAGAAGCG
CATCCTCGGGCTGACGCGCCAGGTGTCTATGTTGGGGAAAAGAAAATTTGCTCACTGGGT
TTACGTATTCGACGCGGTTGTTCATTCCACGGTCTGGCATTAAACGTCAATATGGATCTT
TCACCATTTTTACGTATTAATCCTTGTGGGTATGCCGGAATGGAAATGGCTAAAATATCA
CAATGGAAACCCGAAGCGACGACTAATAATATTGCTCCACGTTTACTGGAAAATATTTTA
GCGCTACTAAACAATCCGGACTTCGAATATATTACCGCTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:213
Protein Molecular Weight:23882
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Octanoyltransferase
MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHYPVFTQGQAGKAEHILM
PGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEA
HPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDLSPFLRINPCGYAGMEMAKIS
QWKPEATTNNIAPRLLENILALLNNPDFEYITA
References
External Links:
ResourceLink
Uniprot ID:P60720
Uniprot Name:LIPB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674728
Ecogene ID:EG11591
Ecocyc:EG11591
ColiBase:b0630
Kegg Gene:b0630
EchoBASE ID:EB1549
CCDB:LIPB_ECOLI
BacMap:90111156
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jordan, S. W., Cronan, J. E. Jr (2003). "The Escherichia coli lipB gene encodes lipoyl (octanoyl)-acyl carrier protein:protein transferase." J Bacteriol 185:1582-1589. Pubmed: 12591875
  • Morris, T. W., Reed, K. E., Cronan, J. E. Jr (1995). "Lipoic acid metabolism in Escherichia coli: the lplA and lipB genes define redundant pathways for ligation of lipoyl groups to apoprotein." J Bacteriol 177:1-10. Pubmed: 8002607
  • Nesbitt, N. M., Baleanu-Gogonea, C., Cicchillo, R. M., Goodson, K., Iwig, D. F., Broadwater, J. A., Haas, J. A., Fox, B. G., Booker, S. J. (2005). "Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase." Protein Expr Purif 39:269-282. Pubmed: 15642479
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Reed, K. E., Cronan, J. E. Jr (1993). "Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes." J Bacteriol 175:1325-1336. Pubmed: 8444795
  • Zhao, X., Miller, J. R., Cronan, J. E. (2005). "The reaction of LipB, the octanoyl-[acyl carrier protein]:protein N-octanoyltransferase of lipoic acid synthesis, proceeds through an acyl-enzyme intermediate." Biochemistry 44:16737-16746. Pubmed: 16342964