| Identification |
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| Name: | 50S ribosomal protein L4 |
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| Synonyms: | Not Available |
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| Gene Name: | rplD |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | translation |
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| Specific Function: | One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.Protein L4 is a both a transcriptional repressor and a translational repressor protein; these two functions are independent of each other. It regulates transcription of the S10 operon (to which L4 belongs) by causing premature termination of transcription within the S10 leader; termination absolutely requires the NusA protein. L4 controls the translation of the S10 operon by binding to its mRNA. The regions of L4 that control regulation (residues 131-210) are different from those required for ribosome assembly (residues 89-103).Forms part of the polypeptide exit tunnel.Can regulate expression from Citrobacter freundii, Haemophilus influenzae, Morganella morganii, Salmonella typhimurium, Serratia marcescens, Vibrio cholerae and Yersinia enterocolitica (but not Pseudomonas aeruginosa) S10 leaders in vitro. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | |
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| Metabolites: | |
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| GO Classification: | | Function |
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| cytosolic large ribosomal subunit | | DNA-templated transcription, termination | | negative regulation of translation | | regulation of transcription, DNA-templated | | response to antibiotic | | RNA binding | | rRNA binding | | structural constituent of ribosome | | translation | | translation repressor activity |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >606
atggaattagtattgaaagacgcgcagagcgcgctgactgtttccgaaactaccttcggt
cgtgatttcaacgaagcgctggttcaccaggttgttgttgcttatgcagctggtgctcgt
cagggtactcgtgctcagaagactcgtgctgaagtaactggttccggtaaaaaaccgtgg
cgccagaaaggcaccggccgtgcgcgttctggttctatcaagagcccgatctggcgttct
ggtggcgtgacctttgctgctcgtccgcaggaccacagtcaaaaagttaacaagaagatg
taccgcggcgcgctgaaaagcatcctgtccgaactggtacgtcaggatcgtctgatcgtt
gtcgagaagttctctgtagaagcgccgaaaactaagctgctggcacagaaactgaaagac
atggctctggaagatgtgctgatcatcaccggtgagctggacgaaaacctgttcctggct
gcgcgcaacctgcacaaggttgacgtacgcgatgcaactggtatcgacccggttagcctg
atcgccttcgacaaagtcgtaatgactgctgatgctgttaagcaagttgaggagatgctg
gcatga |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 201 |
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| Protein Molecular Weight: | 22086 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 2J28 |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >50S ribosomal protein L4
MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPW
RQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIV
VEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLAARNLHKVDVRDATGIDPVSL
IAFDKVVMTADAVKQVEEMLA |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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