Aquaporin Z (P60844)

Identification
Name:Aquaporin Z
Synonyms:
  • Bacterial nodulin-like intrinsic protein
Gene Name:aqpZ
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
transporter activity
Process
establishment of localization
transmembrane transport
transport
Gene Properties
Blattner:b0875
Gene OrientationCounterclockwise
Centisome Percentage:19.71
Left Sequence End914575
Right Sequence End915270
Gene Sequence:
>696 bp
ATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGA
CTTGAACCGCAAGCAGAAGATGCGCCGCTTAACAACTATGCTAACGAGCGGCTTACCCAA
CTTAACCAACAGCAGCGCTGTGACGCGTTTATTCAGTTTGCCAGCGATGTTATGTTGACG
CGCTGTGAGCTGACCAGCGAGGCGAAGGCGGCAGAAGCTGCAATACGTCTGTGTAAAGAG
CTGGGAGATCAGTCGGTCGTGATTAGCGGTGACACGAGGCTGGAGGAATTGGGGATTAGC
GAACGTTTGCAGCAGGAATGCAATGCCGTTGTTTGGGATCCGGCGAAAGGTGCCGAGAAT
ATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAA
TCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCG
GAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCA
GAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATT
AGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGGCCCG
GTGAAAGCGGTGTATCTGATTATTGAGGATTGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:231
Protein Molecular Weight:23703
Protein Theoretical pI:8
PDB File:1RC2
Signaling Regions:
  • None
Transmembrane Regions:
  • 9-29
  • 34-54
  • 82-102
  • 131-151
  • 156-176
  • 202-222
Protein Sequence:
>Aquaporin Z
MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGG
HFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNG
YGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPV
TNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD
References
External Links:
ResourceLink
Uniprot ID:P60844
Uniprot Name:AQPZ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674452
PDB ID:1RC2
Ecogene ID:EG13270
Ecocyc:EG13270
ColiBase:b0875
Kegg Gene:b0875
EchoBASE ID:EB3055
CCDB:AQPZ_ECOLI
BacMap:16128843
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borgnia, M. J., Kozono, D., Calamita, G., Maloney, P. C., Agre, P. (1999). "Functional reconstitution and characterization of AqpZ, the E. coli water channel protein." J Mol Biol 291:1169-1179. Pubmed: 10518952
  • Calamita, G. (2000). "The Escherichia coli aquaporin-Z water channel." Mol Microbiol 37:254-262. Pubmed: 10931322
  • Calamita, G., Bishai, W. R., Preston, G. M., Guggino, W. B., Agre, P. (1995). "Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli." J Biol Chem 270:29063-29066. Pubmed: 7493926
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Delamarche, C., Thomas, D., Rolland, J. P., Froger, A., Gouranton, J., Svelto, M., Agre, P., Calamita, G. (1999). "Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy." J Bacteriol 181:4193-4197. Pubmed: 10400575
  • Fushimi, K., Bai, L., Marumo, F., Sasaki, S. (1997). "Isolation of a gene encoding nodulin-like intrinsic protein of Escherichia coli." Biochem Mol Biol Int 41:995-1003. Pubmed: 9137831
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pohl, P., Saparov, S. M., Borgnia, M. J., Agre, P. (2001). "Highly selective water channel activity measured by voltage clamp: analysis of planar lipid bilayers reconstituted with purified AqpZ." Proc Natl Acad Sci U S A 98:9624-9629. Pubmed: 11493683
  • Ringler, P., Borgnia, M. J., Stahlberg, H., Maloney, P. C., Agre, P., Engel, A. (1999). "Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography." J Mol Biol 291:1181-1190. Pubmed: 10518953
  • Savage, D. F., Egea, P. F., Robles-Colmenares, Y., O'Connell, J. D. 3rd, Stroud, R. M. (2003). "Architecture and selectivity in aquaporins: 2.5 a X-ray structure of aquaporin Z." PLoS Biol 1:E72. Pubmed: 14691544
  • Scheuring, S., Ringler, P., Borgnia, M., Stahlberg, H., Muller, D. J., Agre, P., Engel, A. (1999). "High resolution AFM topographs of the Escherichia coli water channel aquaporin Z." EMBO J 18:4981-4987. Pubmed: 10487750