ADP-L-glycero-D-manno-heptose-6-epimerase (P67910)

Identification
Name:ADP-L-glycero-D-manno-heptose-6-epimerase
Synonyms:
  • ADP-L-glycero-beta-D-manno-heptose-6-epimerase
  • ADP-glyceromanno-heptose 6-epimerase
  • ADP-hep 6-epimerase
  • AGME
Gene Name:hldD
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose
Cellular Location:Not Available
SMPDB Pathways:
  • ADP-L-glycero-Beta-D-manno-heptose biosynthesis PW002095
  • Lipopolysaccharide biosynthesis PW000831
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0ADP-D-Glycero-D-manno-heptose ↔ 1.0ADP-L-Glycero-D-manno-heptose
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0Thumb
1.0ADP-D-Glycero-D-manno-heptose → 1.0ADP-L-glycero-beta-D-manno-heptose
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb
1.0ADP-D-Glycero-D-manno-heptose ↔ 1.0ADP-L-Glycero-D-manno-heptose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20112ADP-D-Glycero-D-manno-heptoseMetaboCard
ECMDB24180ADP-L-glycero-beta-D-manno-heptoseMetaboCard
ECMDB20113ADP-L-Glycero-D-manno-heptoseMetaboCard
GO Classification:
Function
ADP-glyceromanno-heptose 6-epimerase activity
binding
catalytic activity
coenzyme binding
cofactor binding
isomerase activity
NADP or NADPH binding
nucleotide binding
racemase and epimerase activity
racemase and epimerase activity, acting on carbohydrates and derivatives
Process
carbohydrate metabolic process
cellular metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3619
Gene OrientationClockwise
Centisome Percentage:81.73
Left Sequence End3792010
Right Sequence End3792942
Gene Sequence:
>933 bp
ATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTC
GGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCG
GCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCA
CGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATT
CCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCC
TGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGAC
ACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTAC
GACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATC
ATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATT
AAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGC
ATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAG
CTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCA
GGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCC
GGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCC
GACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTG
GATACGGCGGGCGCACGAGTACTGGAAAACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:310
Protein Molecular Weight:34893
Protein Theoretical pI:5
PDB File:1EQ2
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>ADP-L-glycero-D-manno-heptose-6-epimerase
MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVDLNIADYMDKEDFLIQI
MAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLHYCLEREIPFLYASSAATY
GGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANSQIVGFRYFNVYGPREGHKGS
MASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVADVNLWFLENGVSGIFNLGTGRA
ESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFTQADLTNLRAAGYDKPFKTVAEGVT
EYMAWLNRDA
References
External Links:
ResourceLink
Uniprot ID:P67910
Uniprot Name:HLDD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674277
PDB ID:1EQ2
Ecogene ID:EG10838
Ecocyc:EG10838
ColiBase:b3619
Kegg Gene:b3619
EchoBASE ID:EB0831
CCDB:HLDD_ECOLI
BacMap:16131490
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Coleman, W. G. Jr (1983). "The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis." J Biol Chem 258:1985-1990. Pubmed: 6337148
  • Deacon, A. M., Ni, Y. S., Coleman, W. G. Jr, Ealick, S. E. (2000). "The crystal structure of ADP-L-glycero-D-mannoheptose 6-epimerase: catalysis with a twist." Structure 8:453-462. Pubmed: 10896473
  • Ding, L., Seto, B. L., Ahmed, S. A., Coleman, W. G. Jr (1994). "Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosugar epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase." J Biol Chem 269:24384-24390. Pubmed: 7929099
  • Ding, L., Zhang, Y., Deacon, A. M., Ealick, S. E., Ni, Y., Sun, P., Coleman, W. G. Jr (1999). "Crystallization and preliminary X-ray diffraction studies of the lipopolysaccharide core biosynthetic enzyme ADP-L-glycero-D-mannoheptose 6-epimerase from Escherichia coli K-12." Acta Crystallogr D Biol Crystallogr 55:685-688. Pubmed: 10089470
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kneidinger, B., Marolda, C., Graninger, M., Zamyatina, A., McArthur, F., Kosma, P., Valvano, M. A., Messner, P. (2002). "Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli." J Bacteriol 184:363-369. Pubmed: 11751812
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Morrison, J. P., Tanner, M. E. (2007). "A two-base mechanism for Escherichia coli ADP-L-glycero-D-manno-heptose 6-epimerase." Biochemistry 46:3916-3924. Pubmed: 17316025
  • Ni, Y., McPhie, P., Deacon, A., Ealick, S., Coleman, W. G. Jr (2001). "Evidence that NADP+ is the physiological cofactor of ADP-L-glycero-D-mannoheptose 6-epimerase." J Biol Chem 276:27329-27334. Pubmed: 11313358
  • Pegues, J. C., Chen, L. S., Gordon, A. W., Ding, L., Coleman, W. G. Jr (1990). "Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene." J Bacteriol 172:4652-4660. Pubmed: 2198271
  • Raina, S., Georgopoulos, C. (1991). "The htrM gene, whose product is essential for Escherichia coli viability only at elevated temperatures, is identical to the rfaD gene." Nucleic Acids Res 19:3811-3819. Pubmed: 1861974
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842