Identification
Name:Endonuclease V
Synonyms:Not Available
Gene Name:nfi
Enzyme Class:
Biological Properties
General Function:DNA repair
Specific Function:DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Has a wide substrate spectrum. In addition to deoxyinosine-containing DNA, the enzyme cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures. Participates in the excision repair of hypoxanthine and xanthine (deaminated adenine and guanine) in DNA. It thereby reduces the mutagenic effects of nitrous acid by attacking lesions caused by nitrosative deamination. Also active on inosines in single- and double-stranded RNA. May cleave tRNA(Arg2), which contains inosine at the wobble position.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
cytoplasm
deoxyribonuclease V activity
DNA repair
magnesium ion binding
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>672
atggatctcgcgtcattacgcgctcaacaaatcgaactggcttcttctgtgatccgcgag
gatcgactcgataaagatccaccggatctgatcgccggagccgatgtcgggtttgagcag
ggcggagaagtgacgcgagcggcgatggtgctgctgaaatatccctcgcttgagctggtc
gagtataaagttgcccgcatcgccaccaccatgccttacattccaggttttctttccttc
cgcgaatatcctgcgctgctggcagcgtgggagatgctgtcgcaaaagccggatttagtg
tttgtcgatggtcatgggatctcgcatcctcgccgtcttggcgtcgccagccattttggc
ttattggtggatgtgccgaccattggcgtggcgaaaaaacggctctgcggtaaattcgaa
ccgctctccagcgaaccgggcgcgctggccccactgatggataaaggcgagcagctggcc
tgggtctggcgcagcaaagcgcgctgtaacccgttgtttatcgctaccggccatcgggtc
agcgtggacagcgcgctggcgtgggtacaacgctgcatgaaaggctatcgtctgccggag
ccaacgcgctgggcggacgcggtggcctcggaacgtccggcgttcgtgcgctatacagca
aatcagccctaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:223
Protein Molecular Weight:24672
Protein Theoretical pI:Not Available
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>Endonuclease V
MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEVTRAAMVLLKYPSLELV
EYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFVDGHGISHPRRLGVASHFG
LLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLAWVWRSKARCNPLFIATGHRV
SVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRYTANQP
References
External Links:
ResourceLink
Uniprot ID:P68739
Uniprot Name:NFI_ECOLI
CCDB:NFI_ECOLI
General Reference:Not Available