ECMDB: Fructose-like phosphotransferase enzyme IIB component 1 (P69808)

Identification

Name:

Fructose-like phosphotransferase enzyme IIB component 1

Synonyms:

PTS system fructose-like EIIB component 1

Gene Name:

fryB

Enzyme Class:

2.7.1.69

Biological Properties

General Function:

Involved in sugar:hydrogen symporter activity

Specific Function:

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

Galactose metabolism PW000821
Starch and sucrose metabolism PW000941

KEGG Pathways:

Amino sugar and nucleotide sugar metabolism ec00520
Ascorbate and aldarate metabolism ec00053
Fructose and mannose metabolism ec00051
Galactose metabolism ec00052
Glycolysis / Gluconeogenesis ec00010
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Pentose phosphate pathway ec00030
Starch and sucrose metabolism ec00500

KEGG Reactions:

1.0Protein N(pi)-phospho-L-histidine

1.0Sugar

↔

1.0Protein histidine

1.0

1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard

Complex Reactions:

1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine

1.0

→

1.0protein EIIB

1.0sugar phosphate

1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine + 1.0Sucrose → 1.0protein EIIB + 1.0sugar phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00258	Sucrose	MetaboCard

GO Classification:

Component
cell part
membrane
Function
carbohydrate transmembrane transporter activity
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport

Gene Properties

Blattner:

b2387

Gene Orientation

Counterclockwise

Centisome Percentage:

54.01

Left Sequence End

2505938

Right Sequence End

2506264

Gene Sequence:

>327 bp
ATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCA
ACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCA
CAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAG
AAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGT
CTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGG
CAGGAAATACGCAGTAATAACAAATAA

Protein Properties

Pfam Domain Function:

PTS_IIB (PF02302 )

Protein Residues:

108

Protein Molecular Weight:

11735

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Fructose-like phosphotransferase enzyme IIB component 1
MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGADGIQNRLTAQDIAEATI
IIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEMIASEQQ

References

External Links:

Resource	Link
Uniprot ID:	P69808
Uniprot Name:	PTFB1_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062283
Ecogene ID:	EG14155
Ecocyc:	EG14155
ColiBase:	b2387
Kegg Gene:	b2387
EchoBASE ID:	EB3907
CCDB:	PTFB1_ECOLI
BacMap:	16130319

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837