| Identification |
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| Name: | DnaA regulatory inactivator Hda |
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| Synonyms: | Not Available |
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| Gene Name: | hda |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | negative regulation of DNA-dependent DNA replication initiation |
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| Specific Function: | Mediates the interactions of DNA replication initiator protein DnaA with DNA polymerase subunit beta sliding clamp (dnaN). Stimulates hydrolysis of ATP-DnaA to ADP-DnaA, rendering DnaA inactive for reinitiation, a process called regulatory inhibition of DnaA or RIDA. ADP-binding activates Hda to hydrolyze DnaA-ATP; Hda monomers bind to ADP with about 200-fold greater affinity than for ATP. RIDA function can be genetically separated from viability, suggesting this protein has another function as well.Suppresses the toxic effect of overexpressing a TrfA N-terminal 163 residue fragment. Inhibits inner membrane-associated plasmid IncP-alpha RK2 replication probably by interacting with plasmid-encoded TrfA. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Metabolites: | |
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| GO Classification: | | Function |
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| DNA replication | | DNA replication initiation | | identical protein binding | | membrane | | negative regulation of DNA-dependent DNA replication initiation | | nucleotide binding | | plasma membrane | | sequence-specific DNA binding |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >702
ctgaacacaccggcacagctctctttgccactttatcttcctgacgacgaaacctttgca
agtttctggccgggggataactcctctttactggccgcgctgcaaaacgtgctgcgtcag
gaacatagcggttacatctatctctgggcacgcgaaggcgcggggcgcagccatctgctg
cacgcggcttgcgcggaattgtcgcagcgtggcgatgcggtgggctatgtcccgctggat
aaacgcacctggtttgttccggaagtgctcgacggtatggagcatttgtcgctggtctgt
atcgacaacattgagtgtattgcaggcgatgagttgtgggagatggcgattttcgatctc
tacaatcgaattctggaatcgggcaaaacacggttgttgatcaccggcgatcgtccaccg
cggcagttgaatctgggattaccggatctcgcgtcgcgactcgactgggggcagatctac
aaattgcagccactttctgatgaagataagttgcaggcgctacagttacgcgcgcgtttg
cgtggttttgaactgccggaagatgtggggcgtttcttgctgaagcggctcgacagagaa
atgcgcacgctatttatgacgttggatcagttggatcgtgcgtcgattaccgcgcaacgt
aagctgaccattccgtttgtgaaagaaattctgaagttgtag |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 233 |
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| Protein Molecular Weight: | 26632 |
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| Protein Theoretical pI: | Not Available |
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| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >DnaA regulatory inactivator Hda
MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSGYIYLWAREGAGRSHLL
HAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCIDNIECIAGDELWEMAIFDL
YNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARL
RGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILKL |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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