ECMDB: Thiamine kinase (P75948)

Identification

Name:

Thiamine kinase

Synonyms:

Not Available

Gene Name:

thiK

Enzyme Class:

2.7.1.89

Biological Properties

General Function:

Involved in transferase activity, transferring phosphorus-containing groups

Specific Function:

Catalyzes the phosphorylation of thiamine to thiamine phosphate

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Metabolic pathways eco01100
Thiamine metabolism ec00730

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Thiamine ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Thiamine monophosphate
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Thiamine ↔ 1.0ADP + 1.0Thiamine monophosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Thiamine ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Thiamine monophosphate
ReactionCard

1.0

→

1.0

1.0Thiamine + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Thiamine monophosphate + 1.0ADP
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Thiamine → 1.0ADP + 1.0Thiamine monophosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00235	Thiamine	MetaboCard
ECMDB02666	Thiamine monophosphate	MetaboCard

GO Classification:

Function
catalytic activity
kinase activity
thiamin kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular metabolic process
metabolic process
phosphate metabolic process
phosphorus metabolic process
phosphorylation

Gene Properties

Blattner:

b1106

Gene Orientation

Clockwise

Centisome Percentage:

25.06

Left Sequence End

1162483

Right Sequence End

1163307

Gene Sequence:

>825 bp
GTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCG
CAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAA
CATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG
TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCAT
TTATATCTCCGTGACTGGATGGTAGTCGACTATCTGCCCGGCGCGGTAAAAACGTATTTG
CCGGATACCAACGAACTGGCAGGCTTGCTGTATTATCTACATCAACAACCACGTTTTGGC
TGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGCAGCAAAGCGATCCGGCGCGG
CGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAAGCGCGGGAACCACGGCCT
TTACGCTTAAGTCCATTGCATATGGATGTCCACGCCGGAAATTTAGTGCATAGCGCGTCA
GGGTTAAAACTCATCGACTGGGAGTATGCCGGAGATGGTGATATCGCGCTGGAACTGGCG
GCGGTGTGGGTGGAAAATACTGAACAGCACCGGCAATTGGTCAATGACTATGCCACTCGC
GCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTG
ATGCTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATC
AGGCTGGCCGATGACACCTGGCGGCAGCTATTAATAAAACAATAA

Protein Properties

Pfam Domain Function:

APH (PF01636 )

Protein Residues:

274

Protein Molecular Weight:

32397

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Thiamine kinase
MPFRSNNPITRDELLSRFFPQYHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPQSA
FLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPDTNELAGLLYYLHQQPRFG
WRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
GLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ

References

External Links:

Resource	Link
Uniprot ID:	P75948
Uniprot Name:	THIK_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062670
Ecogene ID:	EG13432
Ecocyc:	EG13432
ColiBase:	b1106
Kegg Gene:	b1106
EchoBASE ID:	EB3206
CCDB:	THIK_ECOLI
BacMap:	16129069

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Melnick, J., Lis, E., Park, J. H., Kinsland, C., Mori, H., Baba, T., Perkins, J., Schyns, G., Vassieva, O., Osterman, A., Begley, T. P. (2004). "Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase." J Bacteriol 186:3660-3662. Pubmed: 15150256
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232