ECMDB

Identification

Name:

5'-nucleotidase yfbR

Synonyms:

5'-deoxyribonucleotidase
Nucleoside 5'-monophosphate phosphohydrolase

Gene Name:

yfbR

Enzyme Class:

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Nucleotidase that shows strict specificity toward deoxyribonucleoside 5'-monophosphates and does not dephosphorylate 5'-ribonucleotides or ribonucleoside 3'-monophosphates. Might be involved in the regulation of all dNTP pools in E.coli

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

Pyrimidine metabolism PW000942

KEGG Pathways:

Metabolic pathways eco01100
Nicotinate and nicotinamide metabolism ec00760
Purine metabolism ec00230
Pyrimidine metabolism ec00240

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine monophosphate + 1.0Water ↔ 1.0Adenosine + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Cytidine monophosphate + 1.0Water ↔ 1.0Cytidine + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Uridine 5'-monophosphate + 1.0Water ↔ 1.0Uridine + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Inosinic acid + 1.0Water ↔ 1.0Inosine + 1.0Phosphate
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1.0

↔

1.0

1.0Guanosine monophosphate + 1.0Water ↔ 1.0Guanosine + 1.0Phosphate
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1.0

↔

1.0

1.05-Thymidylic acid + 1.0Water ↔ 1.0Thymidine + 1.0Phosphate
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1.0

↔

1.0

1.0dCMP + 1.0Water ↔ 1.0Deoxycytidine + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.02'-Deoxyguanosine 5'-monophosphate + 1.0Water ↔ 1.0Deoxyguanosine + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Deoxyadenosine monophosphate + 1.0Water ↔ 1.0Deoxyadenosine + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Nicotinamide ribotide + 1.0Water ↔ 1.0Nicotinamide riboside + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Xanthylic acid + 1.0Water ↔ 1.0Xanthosine + 1.0Phosphate
ReactionCard

1.05'-Ribonucleotide

1.0

↔

1.0

1.05'-Ribonucleotide + 1.0Water ↔ 1.0Ribonucleoside + 1.0Phosphate
ReactionCard

1.0Deoxynucleoside 5'-phosphate

1.0

↔

1.0Deoxynucleoside

1.0

1.0Deoxynucleoside 5'-phosphate + 1.0Water ↔ 1.0Deoxynucleoside + 1.0Phosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0dCMP + 1.0Water ↔ 1.0Deoxycytidine + 1.0Phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01044	2'-Deoxyguanosine 5'-monophosphate	MetaboCard
ECMDB01227	5-Thymidylic acid	MetaboCard
ECMDB00050	Adenosine	MetaboCard
ECMDB00045	Adenosine monophosphate	MetaboCard
ECMDB00089	Cytidine	MetaboCard
ECMDB00095	Cytidine monophosphate	MetaboCard
ECMDB01202	dCMP	MetaboCard
ECMDB00101	Deoxyadenosine	MetaboCard
ECMDB00905	Deoxyadenosine monophosphate	MetaboCard
ECMDB00014	Deoxycytidine	MetaboCard
ECMDB00085	Deoxyguanosine	MetaboCard
ECMDB00071	Deoxyinosine	MetaboCard
ECMDB00012	Deoxyuridine	MetaboCard
ECMDB06555	DIMP	MetaboCard
ECMDB01409	dUMP	MetaboCard
ECMDB00133	Guanosine	MetaboCard
ECMDB01397	Guanosine monophosphate	MetaboCard
ECMDB21380	Inorganic phosphate	MetaboCard
ECMDB00195	Inosine	MetaboCard
ECMDB04085	Inosinic acid	MetaboCard
ECMDB00855	Nicotinamide riboside	MetaboCard
ECMDB00229	Nicotinamide ribotide	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB00273	Thymidine	MetaboCard
ECMDB00296	Uridine	MetaboCard
ECMDB00288	Uridine 5'-monophosphate	MetaboCard
ECMDB00494	Water	MetaboCard
ECMDB00299	Xanthosine	MetaboCard
ECMDB01554	Xanthylic acid	MetaboCard

GO Classification:

Function
catalytic activity

Gene Properties

Blattner:

b2291

Gene Orientation

Clockwise

Centisome Percentage:

51.88

Left Sequence End

2406884

Right Sequence End

2407483

Gene Sequence:

>600 bp
ATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTC
TCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGG
TTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGT
ATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGC
CTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGAC
GAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAAC
GAACCGGCAGATCATCTGGCGATCTATCTCGAATTGCTCAGCCATCTGCATTTTTCGCTG
GGAGAGGGGACCGTTCCTGCGCGAAGAATCGACAGTTTGCGGCAAAAAACACTGACGGCG
CTGTGGCAATGGTTACCAGAGTTTGTTGCGCGTTGTCGTCAGTATGACAGCTTTGGTTTT
TACGCGGCACTAAGCCAGTTATTGCTGGTGTTAGTGGAGTGCGACCACCAAAACAGATAA

Protein Properties

Pfam Domain Function:

HD (PF01966 )

Protein Residues:

199

Protein Molecular Weight:

22708

Protein Theoretical pI:

PDB File:

1WPH

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>5'-nucleotidase yfbR
MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHALAAIKNRKFGGNVNAER
IALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQKLVDMVPEELRDIFAPLI
DEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAKTRLEATLEARRSQEMDYFME
IFVPSFHLSLDEISQDSPL

References

External Links:

Resource	Link
Uniprot ID:	P76491
Uniprot Name:	YFBR_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1651490
PDB ID:	1WPH
Ecogene ID:	EG14102
Ecocyc:	EG14102
ColiBase:	b2291
Kegg Gene:	b2291
EchoBASE ID:	EB3855
CCDB:	YFBR_ECOLI
BacMap:	16130226

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Proudfoot, M., Kuznetsova, E., Brown, G., Rao, N. N., Kitagawa, M., Mori, H., Savchenko, A., Yakunin, A. F. (2004). "General enzymatic screens identify three new nucleotidases in Escherichia coli. Biochemical characterization of SurE, YfbR, and YjjG." J Biol Chem 279:54687-54694. Pubmed: 15489502

5'-nucleotidase yfbR (P76491)