ECMDB: Glutaminase 1 (P77454)

Identification

Name:

Glutaminase 1

Synonyms:

Not Available

Gene Name:

glsA1

Enzyme Class:

3.5.1.2

Biological Properties

General Function:

Involved in glutaminase activity

Specific Function:

L-glutamine + H(2)O = L-glutamate + NH(3)

Cellular Location:

Cytoplasmic

SMPDB Pathways:

D-Glutamine and D-glutamate metabolism PW000769
L-glutamate metabolism PW000789
L-glutamate metabolism II PW001886
Nitrogen metabolism PW000755

KEGG Pathways:

Alanine, aspartate and glutamate metabolism ec00250
Arginine and proline metabolism ec00330
D-Glutamine and D-glutamate metabolism ec00471
Metabolic pathways eco01100
Nitrogen metabolism ec00910

KEGG Reactions:

1.0

↔

1.0

1.0L-Glutamine + 1.0Water ↔ 1.0L-Glutamate + 1.0Ammonia
ReactionCard

1.0

↔

1.0

1.0D-Glutamine + 1.0Water ↔ 1.0D-Glutamic acid + 1.0Ammonia
ReactionCard

1.0

→

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamate + 1.0Ammonia
ReactionCard

SMPDB Reactions:

1.0

→

1.0L-Glutamic acid

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamic acid + 1.0Ammonia + 1.0L-Glutamate
ReactionCard

1.0

→

1.0

1.0D-Glutamine + 1.0Water → 1.0D-Glutamic acid + 1.0Ammonia
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0L-Glutamine + 1.0Water ↔ 1.0L-Glutamate + 1.0Ammonia
ReactionCard

1.0

→

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamate + 1.0Ammonia
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamate + 1.0Ammonium
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB03339	D-Glutamic acid	MetaboCard
ECMDB03423	D-Glutamine	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB00641	L-Glutamine	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
glutaminase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
glutamine metabolic process
metabolic process

Gene Properties

Blattner:

b0485

Gene Orientation

Clockwise

Centisome Percentage:

11.01

Left Sequence End

510865

Right Sequence End

511797

Gene Sequence:

>933 bp
ATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTC
GGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCG
GCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCA
CGGGAAAATATCGTTTATCAGTGCTGGGAGCGTTTTTGCCAGGAACTGGGTAAGCAAATT
CCAGTGGCGATGACCCTGGAAAAGAATATGCCGATCGGTTCGGGCTTAGGCTCCAGTGCC
TGTTCGGTGGTCGCGGCGCTGATGGCGATGAATGAACACTGCGGCAAGCCGCTTAATGAC
ACTCGTTTGCTGGCTTTGATGGGCGAGCTGGAAGGCCGTATCTCCGGCAGCATTCATTAC
GACAACGTGGCACCGTGTTTTCTCGGTGGTATGCAGTTGATGATCGAAGAAAACGACATC
ATCAGCCAGCAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATT
AAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGC
ATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAG
CTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCA
GGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCC
GGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCC
GACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTG
GATACGGCGGGCGCACGAGTACTGGAAAACTAA

Protein Properties

Pfam Domain Function:

Glutaminase (PF04960 )

Protein Residues:

310

Protein Molecular Weight:

32903

Protein Theoretical pI:

PDB File:

1U60

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Glutaminase 1
MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVAIVTCDGNVYSAGDSDY
RFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVIALELHGGKPLSPLVNAGA
IATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQSEQTTNFHNRAIAWLLYSAGY
LYCDAMEACDVYTRQCSTLLNTIELATLGATLAAGGVNPLTHKRVLQADNVPYILAEMMM
EGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGIAAFSPPLDEDGNSVRGQKMVASVA
KQLGYNVFKG

References

External Links:

Resource	Link
Uniprot ID:	P77454
Uniprot Name:	GLSA1_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674277
PDB ID:	1U60
Ecogene ID:	EG13247
Ecocyc:	EG13247
ColiBase:	b0485
Kegg Gene:	b0485
EchoBASE ID:	EB3036
CCDB:	GLSA1_ECOLI
BacMap:	16128469

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842