Identification
Name:Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
Synonyms:
  • Polymyxin resistance protein pmrF
  • Undecaprenyl-phosphate Ara4FN transferase
  • Ara4FN transferase
Gene Name:arnC
Enzyme Class:
Biological Properties
General Function:Involved in phosphotransferase activity, for other substituted phosphate groups
Specific Function:Catalyzes the transfer of 4-deoxy-4-formamido-L- arabinose from UDP to undecaprenyl phosphate. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
  • Amino sugar and nucleotide sugar metabolism ec00520
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Undecaprenyl phosphate alpha-L-Ara4FN+1.0Thumb+1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0di-trans,octa-cis-undecaprenyl phosphate1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.04-deoxy-4-formamido-α-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate+1.0Thumb
1.0Uridine 5''-diphospho-{beta}-4-deoxy-4-formamido-L-arabinose + 1.0Di-trans,poly-cis-undecaprenyl phosphate → 1.04-deoxy-4-formamido-α-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + 1.0Uridine 5'-diphosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB231004-Amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphateMetaboCard
ECMDB242414-deoxy-4-formamido-α-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphateMetaboCard
ECMDB20310Di-trans,poly-cis-undecaprenyl phosphateMetaboCard
ECMDB23199UDP-4-Deoxy-4-formamido-beta-L-arabinoseMetaboCard
ECMDB20208Undecaprenyl phosphateMetaboCard
ECMDB21365Undecaprenyl phosphate-4-amino-4-formyl-L-arabinoseMetaboCard
ECMDB21367Uridine 5''-diphospho-{beta}-4-deoxy-4-formamido-L-arabinoseMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b2254
Gene OrientationClockwise
Centisome Percentage:50.98
Left Sequence End2365093
Right Sequence End2366061
Gene Sequence:
>969 bp
ATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTG
AAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGC
TGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGAT
CTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATG
GTGTTCCAGAACTACGCGTTGTATCCGCATATGACGGTTTACGACAACATGGCGTTTGGT
CTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAA
ATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGT
CAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAA
CCGCTCTCTAACCTTGATGCCAAGCTGCGCGTGCAAATGCGCGCAGAGATCAGCAAGCTG
CATCAGAAACTGAACACCACCATGATCTACGTGACCCACGATCAGACCGAAGCGATGACC
ATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCAGCAAGTAGGTGCGCCGAAA
ACCGTTTATAACCAACCCGCGAATATGTTTGTTTCCGGATTTATTGGATCACCAGCGATG
AATTTTATTCGCGGCACGATCGATGGCGATAAATTCGTTACGGAAACGCTTAAATTAACC
ATTCCCGAAGAGAAATTAGCGGTTCTGAAAACACAGGAAAGTTTGCATAAGCCCATCGTG
ATGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCC
AAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGGC
ACGAGTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:322
Protein Molecular Weight:36339
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 236-256
  • 270-290
Protein Sequence:
>Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEILLIDDGSSDNSAHMLVE
ASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDADLQNPPEEIPRLVAKADEG
YDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYGCMLRAYRRHIVDAMLHCHER
STFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLINLMYDLVTCLTTTPLRMLSLLG
SIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYT
DVRARPRYFVQQVIRPSSKENE
References
External Links:
ResourceLink
Uniprot ID:P77757
Uniprot Name:ARNC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742155
Ecogene ID:EG14090
Ecocyc:EG14090
ColiBase:b2254
Kegg Gene:b2254
EchoBASE ID:EB3843
CCDB:ARNC_ECOLI
BacMap:16130189
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Breazeale, S. D., Ribeiro, A. A., McClerren, A. L., Raetz, C. R. (2005). "A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose." J Biol Chem 280:14154-14167. Pubmed: 15695810
  • Breazeale, S. D., Ribeiro, A. A., Raetz, C. R. (2002). "Oxidative decarboxylation of UDP-glucuronic acid in extracts of polymyxin-resistant Escherichia coli. Origin of lipid a species modified with 4-amino-4-deoxy-L-arabinose." J Biol Chem 277:2886-2896. Pubmed: 11706007
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837
  • Yan, A., Guan, Z., Raetz, C. R. (2007). "An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli." J Biol Chem 282:36077-36089. Pubmed: 17928292