| Identification |
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| Name: | CRISPR-associated endonuclease Cas1 |
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| Synonyms: | Not Available |
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| Gene Name: | ygbT |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | maintenance of CRISPR repeat elements |
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| Specific Function: | CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The Cas1-Cas2 complex is involved in CRISPR adaptation, the first stage of CRISPR immunity, being required for the addition/removal of CRISPR spacers. Expression of Cas1-Cas2 in a strain lacking both genes permits spacer acquisition (PubMed:24793649). Endonucleolytically cleaves linear ssRNA, ssDNA and short (34 base) dsDNA as well as branched DNA substrates such as Holliday junctions, replication forks and 5'-flap DNA substrates (PubMed:21219465). Non-specifically binds DNA; the Cas1-Cas2 complex preferentially binds CRISPR-locus DNA (PubMed:24793649). Genetic interactions suggest Cas1 interacts with components of the RecBC and RuvB DNA repair systems. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Metabolites: | |
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| GO Classification: | | Function |
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| 5'-flap endonuclease activity | | cellular response to DNA damage stimulus | | crossover junction endodeoxyribonuclease activity | | cytoplasm | | defense response to virus | | DNA binding | | DNA repair | | maintenance of CRISPR repeat elements | | metal ion binding |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >918
atgacctggcttccccttaatcccattccactcaaagatcgcgtctccatgatctttctg
caatatgggcagatcgatgtaatagatggcgcgtttgtacttatcgacaagacagggatc
cgcactcatattcctgttggctcggttgcctgcatcatgctggaacctggtacacgggtt
tcgcatgcagctgtacgcctggctgcgcaagttggaacattgttggtatgggtgggggaa
gcgggcgttcgtgtttatgcttctggtcagcctggaggtgcgcgttcagataagctgctc
tatcaggcaaaacttgctctggatgaagatttgcgtctgaaggtcgtacgtaaaatgttt
gaacttcggtttggagaacctgcgcctgcccggcgctccgtagagcaactcagaggtata
gaaggcagtcgcgtgcgggcaacctacgcacttctggcgaagcaatacggcgtgacatgg
aatggacgtcgctacgatccgaaagactgggaaaagggcgatacgatcaaccaatgcatt
agcgctgcaacttcctgtttatacggcgtaactgaagcggcgatacttgcagctggttat
gcaccagctattgggtttgtgcatacaggaaagcctctttcctttgtttacgatattgca
gacatcattaaatttgacactgttgtaccgaaagcttttgagatagcgcgtcgtaaccct
ggtgagccggaccgggaagtccgtttggcgtgcagggatatttttcgcagtagtaaaaca
ttagccaaattgattccgcttatagaggacgtgcttgccgctggagaaatacaaccgccg
gccccacctgaagatgcacagcctgttgccattccgcttcctgtttcactgggagatgca
ggccatcggagtagctga |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 305 |
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| Protein Molecular Weight: | 33194 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 3NKD |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >CRISPR-associated endonuclease Cas1
MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRV
SHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQAKLALDEDLRLKVVRKMF
ELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTWNGRRYDPKDWEKGDTINQCI
SAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRNP
GEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGEIQPPAPPEDAQPVAIPLPVSLGDA
GHRSS |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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