ECMDB: CRISPR system Cascade subunit CasD (Q46898)

Identification

Name:

CRISPR system Cascade subunit CasD

Synonyms:

Not Available

Gene Name:

casD

Enzyme Class:

Not Available

Biological Properties

General Function:

defense response to virus

Specific Function:

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasCDE alone is also able to form R-loops.

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Metabolites:

ECMDB ID	Name	View

GO Classification:

Function
defense response to virus
protein complex
RNA binding

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>675
atgagatcttatttgatcttgcggcttgctgggccaatgcaagcctgggggcagccgacc
tttgaaggaacgcgacctaccggaagatttccgacccgaagcgggttattagggctactc
ggggcttgtcttgggatccaacgtgatgatacttcttcattacaggcgttatcagagagt
gtgcaatttgcagtgcgctgcgatgaactcattcttgacgatcgtcgtgtgtctgtaacg
gggttgcgtgattaccatacagtccttggagcgcgagaagattaccgtggtttgaaaagt
catgaaacgattcaaacatggcgcgaatatttatgtgatgcctcctttaccgtcgctctc
tggttaacaccccatgcaacgatggttatctcagaacttgaaaaagcagtattaaagcct
cggtatacaccttacctggggcggagaagttgcccactaacacacccgctttttttgggg
acatgtcaggcatcggatcctcagaaggcgctattaaattatgagcccgttggcggcgat
atatatagtgaggaatcagttacagggcatcatttaaaatttacggcgcgcgacgaaccg
atgatcaccttgcctcgacaatttgcttcccgagaatggtatgtgattaaaggaggtatg
gatgtatctcagtaa

Protein Properties

Pfam Domain Function:

Not Available

Protein Residues:

224

Protein Molecular Weight:

25208

Protein Theoretical pI:

Not Available

PDB File:

4QYZ

Signaling Regions:

Not Available

Transmembrane Regions:

Not Available

Protein Sequence:

>CRISPR system Cascade subunit CasD
MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACLGIQRDDTSSLQALSES
VQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHETIQTWREYLCDASFTVAL
WLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLGTCQASDPQKALLNYEPVGGD
IYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKGGMDVSQ

References

External Links:

Resource	Link
Uniprot ID:	Q46898
Uniprot Name:	CAS5_ECOLI
PDB ID:	4QYZ
CCDB:	CAS5_ECOLI

General Reference:

Not Available