Identification
Name:Probable protein deglycase
Synonyms:Not Available
Gene Name:yajL
Enzyme Class:Not Available
Biological Properties
General Function:ribosome biogenesis
Specific Function:Protein deglycase that repairs methylglyoxal- and glyoxal-glycated amino acids and proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteines, arginines and lysines residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) (PubMed:25416785) (By similarity). Displays a covalent chaperone activity with sulfenylated thiol proteins by forming mixed disulfides with members of the thiol proteome, and preferentially with sulfenylated cellular proteins, upon oxidative stress; these mixed disulfides can be subsequently reduced by low-molecular-weight thiols to regenerate YajL and reduced proteins (PubMed:22157000, PubMed:22321799). Involved in biogenesis of ribosomal proteins, probably as a ribosomal protein-folding chaperone. Confers resistance to oxidative stress. The chaperone activity reported for YajL is probably recruited to execute its deglycase activity, to interact with non-native glycated proteins and gain access to partially buried glycated sites.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
cellular response to oxidative stress
hydrolase activity
protein refolding
response to heat
ribosome biogenesis
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>591
atgagcgcatcggcactggtttgcctcgcccctggtagtgaagagactgaagccgtcacc
actatcgatctgctggttcgcggcggtatcaaagtcaccactgccagcgtcgccagcgat
ggtaacctggcgattacctgctcgcgcggcgtgaagctgctggcggatgcgccgctggtc
gaagtggctgatggcgaatatgacgtgatcgtgctgcctggtggcattaaaggcgcggag
tgttttcgcgatagcactctgctggttgaaaccgttaaacagttccaccgttccgggcgt
atcgtcgcggctatttgcgccgcgccagccaccgtgctggtgccgcacgatatcttcccg
attggtaatatgaccggcttcccgacgctgaaagacaaaattcccgccgaacaatggctg
gacaagcgcgtcgtctgggatgcacgggtaaaattgctgaccagccaggggccgggtaca
gctatcgactttggtctgaaaattatcgacctgttggttgggcgtgaaaaagcccatgaa
gtggcatcacaactggtgatggcggcagggatttataattattacgagtag
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:196
Protein Molecular Weight:20776
Protein Theoretical pI:Not Available
PDB File:2AB0
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>Probable protein deglycase
MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLAITCSRGVKLLADAPLV
EVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIVAAICAAPATVLVPHDIFP
IGNMTGFPTLKDKIPAEQWLDKRVVWDARVKLLTSQGPGTAIDFGLKIIDLLVGREKAHE
VASQLVMAAGIYNYYE
References
External Links:
ResourceLink
Uniprot ID:Q46948
Uniprot Name:YAJL_ECOLI
PDB ID:2AB0
CCDB:YAJL_ECOLI
General Reference:Not Available